Tags

Type your tag names separated by a space and hit enter

Cinnamon extract enhances glucose uptake in 3T3-L1 adipocytes and C2C12 myocytes by inducing LKB1-AMP-activated protein kinase signaling.
PLoS One. 2014; 9(2):e87894.Plos

Abstract

We previously demonstrated that cinnamon extract (CE) ameliorates type 1 diabetes induced by streptozotocin in rats through the up-regulation of glucose transporter 4 (GLUT4) translocation in both muscle and adipose tissues. This present study was aimed at clarifying the detailed mechanism(s) with which CE increases the glucose uptake in vivo and in cell culture systems using 3T3-L1 adipocytes and C2C12 myotubes in vitro. Specific inhibitors of key enzymes in insulin signaling and AMP-activated protein kinase (AMPK) signaling pathways, as well as small interference RNA, were used to examine the role of these kinases in the CE-induced glucose uptake. The results showed that CE stimulated the phosphorylation of AMPK and acetyl-CoA carboxylase. An AMPK inhibitor and LKB1 siRNA blocked the CE-induced glucose uptake. We also found for the first time that insulin suppressed AMPK activation in the adipocyte. To investigate the effect of CE on type 2 diabetes in vivo, we further performed oral glucose tolerance tests and insulin tolerance tests in type 2 diabetes model rats administered with CE. The CE improved glucose tolerance in oral glucose tolerance tests, but not insulin sensitivity in insulin tolerance test. In summary, these results indicate that CE ameliorates type 2 diabetes by inducing GLUT4 translocation via the AMPK signaling pathway. We also found insulin antagonistically regulates the activation of AMPK.

Authors+Show Affiliations

Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, Nihon University Graduate School of Bioresource Sciences, Kanagawa, Japan.Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, Nihon University Graduate School of Bioresource Sciences, Kanagawa, Japan.Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, Nihon University Graduate School of Bioresource Sciences, Kanagawa, Japan.School of Pharmacy, Nihon University, Chiba, Japan.Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, Nihon University Graduate School of Bioresource Sciences, Kanagawa, Japan.Department of Food and Nutrition, Japan Women's University, Tokyo, Japan.Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, Nihon University Graduate School of Bioresource Sciences, Kanagawa, Japan.Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, Nihon University Graduate School of Bioresource Sciences, Kanagawa, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24551069

Citation

Shen, Yan, et al. "Cinnamon Extract Enhances Glucose Uptake in 3T3-L1 Adipocytes and C2C12 Myocytes By Inducing LKB1-AMP-activated Protein Kinase Signaling." PloS One, vol. 9, no. 2, 2014, pp. e87894.
Shen Y, Honma N, Kobayashi K, et al. Cinnamon extract enhances glucose uptake in 3T3-L1 adipocytes and C2C12 myocytes by inducing LKB1-AMP-activated protein kinase signaling. PLoS ONE. 2014;9(2):e87894.
Shen, Y., Honma, N., Kobayashi, K., Jia, L. N., Hosono, T., Shindo, K., Ariga, T., & Seki, T. (2014). Cinnamon extract enhances glucose uptake in 3T3-L1 adipocytes and C2C12 myocytes by inducing LKB1-AMP-activated protein kinase signaling. PloS One, 9(2), e87894. https://doi.org/10.1371/journal.pone.0087894
Shen Y, et al. Cinnamon Extract Enhances Glucose Uptake in 3T3-L1 Adipocytes and C2C12 Myocytes By Inducing LKB1-AMP-activated Protein Kinase Signaling. PLoS ONE. 2014;9(2):e87894. PubMed PMID: 24551069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cinnamon extract enhances glucose uptake in 3T3-L1 adipocytes and C2C12 myocytes by inducing LKB1-AMP-activated protein kinase signaling. AU - Shen,Yan, AU - Honma,Natsumi, AU - Kobayashi,Katsuya, AU - Jia,Liu Nan, AU - Hosono,Takashi, AU - Shindo,Kazutoshi, AU - Ariga,Toyohiko, AU - Seki,Taiichiro, Y1 - 2014/02/14/ PY - 2013/09/26/received PY - 2014/01/04/accepted PY - 2014/2/20/entrez PY - 2014/2/20/pubmed PY - 2014/10/22/medline SP - e87894 EP - e87894 JF - PloS one JO - PLoS ONE VL - 9 IS - 2 N2 - We previously demonstrated that cinnamon extract (CE) ameliorates type 1 diabetes induced by streptozotocin in rats through the up-regulation of glucose transporter 4 (GLUT4) translocation in both muscle and adipose tissues. This present study was aimed at clarifying the detailed mechanism(s) with which CE increases the glucose uptake in vivo and in cell culture systems using 3T3-L1 adipocytes and C2C12 myotubes in vitro. Specific inhibitors of key enzymes in insulin signaling and AMP-activated protein kinase (AMPK) signaling pathways, as well as small interference RNA, were used to examine the role of these kinases in the CE-induced glucose uptake. The results showed that CE stimulated the phosphorylation of AMPK and acetyl-CoA carboxylase. An AMPK inhibitor and LKB1 siRNA blocked the CE-induced glucose uptake. We also found for the first time that insulin suppressed AMPK activation in the adipocyte. To investigate the effect of CE on type 2 diabetes in vivo, we further performed oral glucose tolerance tests and insulin tolerance tests in type 2 diabetes model rats administered with CE. The CE improved glucose tolerance in oral glucose tolerance tests, but not insulin sensitivity in insulin tolerance test. In summary, these results indicate that CE ameliorates type 2 diabetes by inducing GLUT4 translocation via the AMPK signaling pathway. We also found insulin antagonistically regulates the activation of AMPK. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24551069/Cinnamon_extract_enhances_glucose_uptake_in_3T3_L1_adipocytes_and_C2C12_myocytes_by_inducing_LKB1_AMP_activated_protein_kinase_signaling_ L2 - http://dx.plos.org/10.1371/journal.pone.0087894 DB - PRIME DP - Unbound Medicine ER -