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Proteomic analysis of hepatic tissue of Cyprinus carpio L. exposed to cyanobacterial blooms in Lake Taihu, China.
PLoS One. 2014; 9(2):e88211.Plos

Abstract

With the rapid development of industry and agriculture and associated pollution, the cyanobacterial blooms in Lake Taihu have become a major threat to aquatic wildlife and human health. In this study, the ecotoxicological effects of cyanobacterial blooms on cage-cultured carp (Cyprinus carpio L.) in Meiliang Bay of Lake Taihu were investigated. Microcystins (MCs), major cyanobacterial toxins, have been detected in carp cultured at different experimental sites of Meiliang Bay. We observed that the accumulation of MCs in carp was closely associated with several environmental factors, including temperature, pH value, and density of cyanobacterial blooms. The proteomic profile of carp liver exposed to cyanobacterial blooms was analyzed using two-dimensional difference in-gel electrophoresis (2D-DIGE) and mass spectrometry. The toxic effects of cyanobacterial blooms on carp liver were similar to changes caused by MCs. MCs were transported into liver cells and induced the excessive production of reactive oxygen species (ROS). MCs and ROS inhibited protein phosphatase and aldehyde dehydrogenase (ALDH), directly or indirectly resulting in oxidative stress and disruption of the cytoskeleton. These effects further interfered with metabolic pathways in the liver through the regulation of series of related proteins. The results of this study indicated that cyanobacterial blooms pose a major threat to aquatic wildlife in Meiliang Bay in Lake Taihu. These results provided evidence of the molecular mechanisms underlying liver damage in carp exposed to cyanobacterial blooms.

Authors+Show Affiliations

Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Ministry of Environmental Protection, Nanjing, People's Republic of China.State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China.Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Ministry of Environmental Protection, Nanjing, People's Republic of China.State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China.Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Ministry of Environmental Protection, Nanjing, People's Republic of China.Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Ministry of Environmental Protection, Nanjing, People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24558380

Citation

Jiang, Jinlin, et al. "Proteomic Analysis of Hepatic Tissue of Cyprinus Carpio L. Exposed to Cyanobacterial Blooms in Lake Taihu, China." PloS One, vol. 9, no. 2, 2014, pp. e88211.
Jiang J, Wang X, Shan Z, et al. Proteomic analysis of hepatic tissue of Cyprinus carpio L. exposed to cyanobacterial blooms in Lake Taihu, China. PLoS One. 2014;9(2):e88211.
Jiang, J., Wang, X., Shan, Z., Yang, L., Zhou, J., & Bu, Y. (2014). Proteomic analysis of hepatic tissue of Cyprinus carpio L. exposed to cyanobacterial blooms in Lake Taihu, China. PloS One, 9(2), e88211. https://doi.org/10.1371/journal.pone.0088211
Jiang J, et al. Proteomic Analysis of Hepatic Tissue of Cyprinus Carpio L. Exposed to Cyanobacterial Blooms in Lake Taihu, China. PLoS One. 2014;9(2):e88211. PubMed PMID: 24558380.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic analysis of hepatic tissue of Cyprinus carpio L. exposed to cyanobacterial blooms in Lake Taihu, China. AU - Jiang,Jinlin, AU - Wang,Xiaorong, AU - Shan,Zhengjun, AU - Yang,Liuyan, AU - Zhou,Junying, AU - Bu,Yuanqin, Y1 - 2014/02/18/ PY - 2013/11/26/received PY - 2014/01/08/accepted PY - 2014/2/22/entrez PY - 2014/2/22/pubmed PY - 2015/1/15/medline SP - e88211 EP - e88211 JF - PloS one JO - PLoS One VL - 9 IS - 2 N2 - With the rapid development of industry and agriculture and associated pollution, the cyanobacterial blooms in Lake Taihu have become a major threat to aquatic wildlife and human health. In this study, the ecotoxicological effects of cyanobacterial blooms on cage-cultured carp (Cyprinus carpio L.) in Meiliang Bay of Lake Taihu were investigated. Microcystins (MCs), major cyanobacterial toxins, have been detected in carp cultured at different experimental sites of Meiliang Bay. We observed that the accumulation of MCs in carp was closely associated with several environmental factors, including temperature, pH value, and density of cyanobacterial blooms. The proteomic profile of carp liver exposed to cyanobacterial blooms was analyzed using two-dimensional difference in-gel electrophoresis (2D-DIGE) and mass spectrometry. The toxic effects of cyanobacterial blooms on carp liver were similar to changes caused by MCs. MCs were transported into liver cells and induced the excessive production of reactive oxygen species (ROS). MCs and ROS inhibited protein phosphatase and aldehyde dehydrogenase (ALDH), directly or indirectly resulting in oxidative stress and disruption of the cytoskeleton. These effects further interfered with metabolic pathways in the liver through the regulation of series of related proteins. The results of this study indicated that cyanobacterial blooms pose a major threat to aquatic wildlife in Meiliang Bay in Lake Taihu. These results provided evidence of the molecular mechanisms underlying liver damage in carp exposed to cyanobacterial blooms. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24558380/Proteomic_analysis_of_hepatic_tissue_of_Cyprinus_carpio_L__exposed_to_cyanobacterial_blooms_in_Lake_Taihu_China_ L2 - https://dx.plos.org/10.1371/journal.pone.0088211 DB - PRIME DP - Unbound Medicine ER -