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Subcellular localization and responses of superoxide dismutase isoforms in local wheat varieties subjected to continuous soil drought.
Plant Physiol Biochem. 2014 Aug; 81:54-60.PP

Abstract

Water is a key factor influencing the yield and quality of crops. One of the parameters of plant biological tolerance to constantly changing environmental conditions is the change of activities and numerous molecular forms of antioxidant enzymes. Two durum (Triticum durum Desf.) wheat varieties contrasting for drought tolerance, such as Barakatli-95 (drought tolerant) and Garagylchyg-2 (drought sensitive) were grown over a wide area in the field. Experiments were carried out to study the effect of soil drought on changes in activities and subcellular localization of superoxide dismutase isoforms. The levels of malondialdehyde, glycine betaine and total proteins were also analyzed. The level of the enzyme activity appeared to depend on the wheat varieties, duration of drought and stages of leaf development. Native polyacrylamide gel electrophoresis (PAGE) revealed the presence of 9 isoenzymes of superoxide dismutase in wheat leaves during drought. Mn-SOD was found in the mitochondrial fractions, Fe-SOD in the chloroplast fraction and Cu/Zn-SOD is localized in all subcellular fractions. Wheat leaves contain three different isoforms of SOD (Mn-, Fe-, Cu/Zn-SOD). Three isoforms of Mn-SOD, one isoform of Fe-SOD and five of Cu/Zn-SOD were observed in wheat leaves using 3 mM KCN and 5 mM H2O2 as selective inhibitors. The expression of Mn-SOD was preferentially enhanced by drought stress. It seems that Mn-SOD isoforms more than SOD ones play a major role in the scavenging of superoxide radicals. The observed data showed that status of antioxidant enzymes such as SOD could provide a meaningful tool for depicting drought tolerance of wheat genotype.

Authors+Show Affiliations

Institute of Botany, Azerbaijan National Academy of Sciences, 40 Badamdar Highway, Baku AZ1073, Azerbaijan. Electronic address: huseynova-i@botany-az.org.Institute of Botany, Azerbaijan National Academy of Sciences, 40 Badamdar Highway, Baku AZ1073, Azerbaijan.Institute of Botany, Azerbaijan National Academy of Sciences, 40 Badamdar Highway, Baku AZ1073, Azerbaijan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24560039

Citation

Huseynova, Irada M., et al. "Subcellular Localization and Responses of Superoxide Dismutase Isoforms in Local Wheat Varieties Subjected to Continuous Soil Drought." Plant Physiology and Biochemistry : PPB, vol. 81, 2014, pp. 54-60.
Huseynova IM, Aliyeva DR, Aliyev JA. Subcellular localization and responses of superoxide dismutase isoforms in local wheat varieties subjected to continuous soil drought. Plant Physiol Biochem. 2014;81:54-60.
Huseynova, I. M., Aliyeva, D. R., & Aliyev, J. A. (2014). Subcellular localization and responses of superoxide dismutase isoforms in local wheat varieties subjected to continuous soil drought. Plant Physiology and Biochemistry : PPB, 81, 54-60. https://doi.org/10.1016/j.plaphy.2014.01.018
Huseynova IM, Aliyeva DR, Aliyev JA. Subcellular Localization and Responses of Superoxide Dismutase Isoforms in Local Wheat Varieties Subjected to Continuous Soil Drought. Plant Physiol Biochem. 2014;81:54-60. PubMed PMID: 24560039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subcellular localization and responses of superoxide dismutase isoforms in local wheat varieties subjected to continuous soil drought. AU - Huseynova,Irada M, AU - Aliyeva,Durna R, AU - Aliyev,Jalal A, Y1 - 2014/02/06/ PY - 2013/12/06/received PY - 2014/01/28/accepted PY - 2014/2/25/entrez PY - 2014/2/25/pubmed PY - 2015/3/31/medline KW - Antioxidant enzymes KW - Drought KW - Glycine betaine KW - Lipid peroxidation KW - Superoxide dismutase KW - Triticum durum Desf. SP - 54 EP - 60 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol. Biochem. VL - 81 N2 - Water is a key factor influencing the yield and quality of crops. One of the parameters of plant biological tolerance to constantly changing environmental conditions is the change of activities and numerous molecular forms of antioxidant enzymes. Two durum (Triticum durum Desf.) wheat varieties contrasting for drought tolerance, such as Barakatli-95 (drought tolerant) and Garagylchyg-2 (drought sensitive) were grown over a wide area in the field. Experiments were carried out to study the effect of soil drought on changes in activities and subcellular localization of superoxide dismutase isoforms. The levels of malondialdehyde, glycine betaine and total proteins were also analyzed. The level of the enzyme activity appeared to depend on the wheat varieties, duration of drought and stages of leaf development. Native polyacrylamide gel electrophoresis (PAGE) revealed the presence of 9 isoenzymes of superoxide dismutase in wheat leaves during drought. Mn-SOD was found in the mitochondrial fractions, Fe-SOD in the chloroplast fraction and Cu/Zn-SOD is localized in all subcellular fractions. Wheat leaves contain three different isoforms of SOD (Mn-, Fe-, Cu/Zn-SOD). Three isoforms of Mn-SOD, one isoform of Fe-SOD and five of Cu/Zn-SOD were observed in wheat leaves using 3 mM KCN and 5 mM H2O2 as selective inhibitors. The expression of Mn-SOD was preferentially enhanced by drought stress. It seems that Mn-SOD isoforms more than SOD ones play a major role in the scavenging of superoxide radicals. The observed data showed that status of antioxidant enzymes such as SOD could provide a meaningful tool for depicting drought tolerance of wheat genotype. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/24560039/Subcellular_localization_and_responses_of_superoxide_dismutase_isoforms_in_local_wheat_varieties_subjected_to_continuous_soil_drought_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(14)00032-1 DB - PRIME DP - Unbound Medicine ER -