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Pneumocystis carinii in sputum. Comparable efficacy of screening stains and determination of cyst density.
Arch Pathol Lab Med. 1988 Aug; 112(8):816-20.AP

Abstract

To determine whether accurate quantification of Pneumocystis carinii cysts in sputum is possible using currently available means and to determine the optimal stain for screening sputum for P carinii, we undertook a comparative evaluation of readily available stains: (1) Diff-Quik, a stain that provides tinctorial detail comparable with Giemsa, (2) toluidine blue, and (3) silver methenamine on simultaneously thawed sputum samples containing P carinii. We also studied the Diff-Quik stain in combination with (4) toluidine blue and (5) silver methenamine. The three stains were comparable for screening purposes. Each disclosed similar numbers of P carinii aggregates on similarly prepared slides. Cysts were difficult to recognize on Diff-Quik due to the negatively staining character of the cyst wall with this stain. The cyst-wall stains, toluidine blue and silver methenamine, clearly stained the cyst wall. The combination of a cyst-wall stain with Diff-Quik allowed visualization of cysts, highlighted by the cyst-wall stain, and aggregate area, delineated by the Diff-Quik, allowing expression of number of cysts per aggregate area, a measure termed cyst density.

Authors+Show Affiliations

Department of Laboratory Medicine, Veterans Administration Medical Center, University of California, San Francisco, School of Medicine 94121.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

2456048

Citation

Blumenfeld, W, and J M. Griffiss. "Pneumocystis Carinii in Sputum. Comparable Efficacy of Screening Stains and Determination of Cyst Density." Archives of Pathology & Laboratory Medicine, vol. 112, no. 8, 1988, pp. 816-20.
Blumenfeld W, Griffiss JM. Pneumocystis carinii in sputum. Comparable efficacy of screening stains and determination of cyst density. Arch Pathol Lab Med. 1988;112(8):816-20.
Blumenfeld, W., & Griffiss, J. M. (1988). Pneumocystis carinii in sputum. Comparable efficacy of screening stains and determination of cyst density. Archives of Pathology & Laboratory Medicine, 112(8), 816-20.
Blumenfeld W, Griffiss JM. Pneumocystis Carinii in Sputum. Comparable Efficacy of Screening Stains and Determination of Cyst Density. Arch Pathol Lab Med. 1988;112(8):816-20. PubMed PMID: 2456048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pneumocystis carinii in sputum. Comparable efficacy of screening stains and determination of cyst density. AU - Blumenfeld,W, AU - Griffiss,J M, PY - 1988/8/1/pubmed PY - 1988/8/1/medline PY - 1988/8/1/entrez SP - 816 EP - 20 JF - Archives of pathology & laboratory medicine JO - Arch Pathol Lab Med VL - 112 IS - 8 N2 - To determine whether accurate quantification of Pneumocystis carinii cysts in sputum is possible using currently available means and to determine the optimal stain for screening sputum for P carinii, we undertook a comparative evaluation of readily available stains: (1) Diff-Quik, a stain that provides tinctorial detail comparable with Giemsa, (2) toluidine blue, and (3) silver methenamine on simultaneously thawed sputum samples containing P carinii. We also studied the Diff-Quik stain in combination with (4) toluidine blue and (5) silver methenamine. The three stains were comparable for screening purposes. Each disclosed similar numbers of P carinii aggregates on similarly prepared slides. Cysts were difficult to recognize on Diff-Quik due to the negatively staining character of the cyst wall with this stain. The cyst-wall stains, toluidine blue and silver methenamine, clearly stained the cyst wall. The combination of a cyst-wall stain with Diff-Quik allowed visualization of cysts, highlighted by the cyst-wall stain, and aggregate area, delineated by the Diff-Quik, allowing expression of number of cysts per aggregate area, a measure termed cyst density. SN - 0003-9985 UR - https://www.unboundmedicine.com/medline/citation/2456048/Pneumocystis_carinii_in_sputum__Comparable_efficacy_of_screening_stains_and_determination_of_cyst_density_ DB - PRIME DP - Unbound Medicine ER -