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In vitro neuraminidase inhibitory activity of four neuraminidase inhibitors against influenza virus isolates in the 2011-2012 season in Japan.

Abstract

The neuraminidase inhibitors oseltamivir phosphate (Tamiflu®), zanamivir (Relenza®), laninamivir octanoate (Inavir®), and peramivir (Rapiacta®) have been available for the treatment of influenza in Japan since 2010. To assess the extent of viral resistance, we measured the 50% inhibitory concentration (IC₅₀) of each drug for influenza virus isolates from the 2011-2012 influenza season. Specimens were obtained from patients prior to treatment. Viral isolation was done using Madine-Darby canine kidney cells, and the type and subtype of influenza A(H1N1)pdm09, A(H3N2), or influenza B were determined by RT-PCR using type- and subtype-specific primers. The IC₅₀ was determined by a neuraminidase inhibition assay using a fluorescent substrate. The lineage of influenza B virus was determined by direct sequencing of the hemagglutinin gene. Influenza A(H3N2) and influenza B viruses were isolated in 283 and 42 patients, respectively, while no influenza A(H1N1)pdm09 virus was isolated. No isolate showed an IC₅₀ value exceeding 50 nM for any of the neuraminidase inhibitors. IC50 values for A(H3N2) were similar between the 2010-2011 and 2011-2012 seasons. In contrast, the IC₅₀ values for influenza B viruses in the 2011-2012 season to the four drugs were significantly lower than those found in the 2010-2011 season. These results indicate that the currently epidemic influenza viruses are susceptible to all four neuraminidase inhibitors, with no trend for IC₅₀ values to increase in Japan at present.

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  • Authors+Show Affiliations

    ,

    Department of Clinical Trials, Center for Advanced Medical Innovation, Kyushu University, Japan; Japan Physicians Association, Tokyo, Japan. Electronic address: hikematsu@camiku.kyushu-u.ac.jp.

    ,

    Japan Physicians Association, Tokyo, Japan.

    ,

    Japan Physicians Association, Tokyo, Japan.

    Japan Physicians Association, Tokyo, Japan.

    Source

    MeSH

    Adolescent
    Adult
    Aged
    Aged, 80 and over
    Animals
    Antiviral Agents
    Child
    Child, Preschool
    Dogs
    Enzyme Inhibitors
    Female
    Humans
    Infant
    Influenza A Virus, H1N1 Subtype
    Influenza A Virus, H3N2 Subtype
    Influenza B virus
    Influenza, Human
    Inhibitory Concentration 50
    Japan
    Madin Darby Canine Kidney Cells
    Male
    Middle Aged
    Neuraminidase
    Orthomyxoviridae
    Young Adult

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    24560563

    Citation

    Ikematsu, Hideyuki, et al. "In vitro Neuraminidase Inhibitory Activity of Four Neuraminidase Inhibitors Against Influenza Virus Isolates in the 2011-2012 Season in Japan." Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, vol. 20, no. 2, 2014, pp. 77-80.
    Ikematsu H, Kawai N, Iwaki N, et al. In vitro neuraminidase inhibitory activity of four neuraminidase inhibitors against influenza virus isolates in the 2011-2012 season in Japan. J Infect Chemother. 2014;20(2):77-80.
    Ikematsu, H., Kawai, N., Iwaki, N., & Kashiwagi, S. (2014). In vitro neuraminidase inhibitory activity of four neuraminidase inhibitors against influenza virus isolates in the 2011-2012 season in Japan. Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, 20(2), pp. 77-80. doi:10.1016/j.jiac.2013.07.002.
    Ikematsu H, et al. In vitro Neuraminidase Inhibitory Activity of Four Neuraminidase Inhibitors Against Influenza Virus Isolates in the 2011-2012 Season in Japan. J Infect Chemother. 2014;20(2):77-80. PubMed PMID: 24560563.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - In vitro neuraminidase inhibitory activity of four neuraminidase inhibitors against influenza virus isolates in the 2011-2012 season in Japan. AU - Ikematsu,Hideyuki, AU - Kawai,Naoki, AU - Iwaki,Norio, AU - Kashiwagi,Seizaburo, Y1 - 2013/12/11/ PY - 2013/03/27/received PY - 2013/06/12/revised PY - 2013/07/03/accepted PY - 2014/2/25/entrez PY - 2014/2/25/pubmed PY - 2014/11/18/medline KW - 50% Inhibitory concentration KW - Influenza KW - Laninamivir KW - Oseltamivir KW - Peramivir KW - Zanamivir SP - 77 EP - 80 JF - Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy JO - J. Infect. Chemother. VL - 20 IS - 2 N2 - The neuraminidase inhibitors oseltamivir phosphate (Tamiflu®), zanamivir (Relenza®), laninamivir octanoate (Inavir®), and peramivir (Rapiacta®) have been available for the treatment of influenza in Japan since 2010. To assess the extent of viral resistance, we measured the 50% inhibitory concentration (IC₅₀) of each drug for influenza virus isolates from the 2011-2012 influenza season. Specimens were obtained from patients prior to treatment. Viral isolation was done using Madine-Darby canine kidney cells, and the type and subtype of influenza A(H1N1)pdm09, A(H3N2), or influenza B were determined by RT-PCR using type- and subtype-specific primers. The IC₅₀ was determined by a neuraminidase inhibition assay using a fluorescent substrate. The lineage of influenza B virus was determined by direct sequencing of the hemagglutinin gene. Influenza A(H3N2) and influenza B viruses were isolated in 283 and 42 patients, respectively, while no influenza A(H1N1)pdm09 virus was isolated. No isolate showed an IC₅₀ value exceeding 50 nM for any of the neuraminidase inhibitors. IC50 values for A(H3N2) were similar between the 2010-2011 and 2011-2012 seasons. In contrast, the IC₅₀ values for influenza B viruses in the 2011-2012 season to the four drugs were significantly lower than those found in the 2010-2011 season. These results indicate that the currently epidemic influenza viruses are susceptible to all four neuraminidase inhibitors, with no trend for IC₅₀ values to increase in Japan at present. SN - 1437-7780 UR - https://www.unboundmedicine.com/medline/citation/24560563/In vitro_neuraminidase_inhibitory_activity_of_four_neuraminidase_inhibitors_against_influenza_virus_isolates_in_the_2011_2012_season_in_Japan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1341-321X(13)00006-8 DB - PRIME DP - Unbound Medicine ER -