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Polygala molluginifolia A. St.-Hil. and Moq. prevent inflammation in the mouse pleurisy model by inhibiting NF-κB activation.
Int Immunopharmacol. 2014 Apr; 19(2):334-41.II

Abstract

This study was conducted to investigate the anti-inflammatory activity of Polygala molluginifolia (Polygalaceae) on the mouse pleurisy model induced by carrageenan. P. molluginifolia is a plant native to southern Brazil that is popularly called "canfora". The Polygala genus is used to treat different pathologies, including inflammatory diseases, in traditional medicine.

MATERIAL AND METHODS

The whole P. molluginifolia plant material was extracted by maceration with 96% ethanol. The crude hydroalcoholic extract (CE) was subjected to chromatographic procedures to produce various derivate fractions, including its aqueous (Aq), ethyl acetate (EtOAc), and hexane (Hex) fractions. Compound 1 (5,3',4'-trihydroxy-6″,6″-dimethylpyrano [2″,3″:7,6] isoflavone) (Iso), which was isolated from the EtOAc fraction, and Compound 2 (rutin) (Rut), which was isolated from the Aq fraction, were identified using ¹H and ¹³C NMR spectroscopy and quantified using an HPLC apparatus.

RESULTS

The CE, the Aq, EtOAc, and Hex fractions, and the isolated compounds Iso and Rut were able to reduce cell migration and exudation. Furthermore, the plant material also decreased the myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities and the nitric oxide (NO(x)), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) levels. In addition, Iso and Rut reduced the TNF-α and IL-1β mRNA expression levels and significantly decreased NF-κB p65 phosphorylation.

CONCLUSION

The results show that P. molluginifolia has a significant anti-inflammatory action and that this effect is due, at least in part, to the presence of Iso and Rut in large amounts. Moreover, this effect was found to be closely related to the inhibitory effects of the isolated compounds on the NF-κB pathway.

Authors+Show Affiliations

Department of Clinical Analyses, Centre of Health Sciences, Federal University of Santa Catarina, Campus Universitário - Trindade, 88040-970 Florianopolis, SC, Brazil.Department of Clinical Analyses, Centre of Health Sciences, Federal University of Santa Catarina, Campus Universitário - Trindade, 88040-970 Florianopolis, SC, Brazil.Department of Clinical Analyses, Centre of Health Sciences, Federal University of Santa Catarina, Campus Universitário - Trindade, 88040-970 Florianopolis, SC, Brazil.Department of Chemistry, Centre of Physical and Mathematical Sciences, Federal University of Santa Catarina, Campus Universitário - Trindade, 88040-970 Florianopolis, SC, Brazil.Department of Chemistry, Centre of Physical and Mathematical Sciences, Federal University of Santa Catarina, Campus Universitário - Trindade, 88040-970 Florianopolis, SC, Brazil.Department of Clinical Analyses, Centre of Health Sciences, Federal University of Santa Catarina, Campus Universitário - Trindade, 88040-970 Florianopolis, SC, Brazil.Department of Chemistry, Centre of Physical and Mathematical Sciences, Federal University of Santa Catarina, Campus Universitário - Trindade, 88040-970 Florianopolis, SC, Brazil.Department of Clinical Analyses, Centre of Health Sciences, Federal University of Santa Catarina, Campus Universitário - Trindade, 88040-970 Florianopolis, SC, Brazil. Electronic address: dalmarco@ccs.ufsc.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24560858

Citation

Arruda-Silva, Fábio, et al. "Polygala Molluginifolia A. St.-Hil. and Moq. Prevent Inflammation in the Mouse Pleurisy Model By Inhibiting NF-κB Activation." International Immunopharmacology, vol. 19, no. 2, 2014, pp. 334-41.
Arruda-Silva F, Nascimento MV, Luz AB, et al. Polygala molluginifolia A. St.-Hil. and Moq. prevent inflammation in the mouse pleurisy model by inhibiting NF-κB activation. Int Immunopharmacol. 2014;19(2):334-41.
Arruda-Silva, F., Nascimento, M. V., Luz, A. B., Venzke, D., Queiroz, G. S., Fröde, T. S., Pizzolatti, M. G., & Dalmarco, E. M. (2014). Polygala molluginifolia A. St.-Hil. and Moq. prevent inflammation in the mouse pleurisy model by inhibiting NF-κB activation. International Immunopharmacology, 19(2), 334-41. https://doi.org/10.1016/j.intimp.2014.02.010
Arruda-Silva F, et al. Polygala Molluginifolia A. St.-Hil. and Moq. Prevent Inflammation in the Mouse Pleurisy Model By Inhibiting NF-κB Activation. Int Immunopharmacol. 2014;19(2):334-41. PubMed PMID: 24560858.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polygala molluginifolia A. St.-Hil. and Moq. prevent inflammation in the mouse pleurisy model by inhibiting NF-κB activation. AU - Arruda-Silva,Fábio, AU - Nascimento,Marcus Vinicius P S, AU - Luz,Ana B G, AU - Venzke,Dalila, AU - Queiroz,Gustavo S, AU - Fröde,Tânia S, AU - Pizzolatti,Moacir G, AU - Dalmarco,Eduardo M, Y1 - 2014/02/20/ PY - 2013/12/13/received PY - 2014/02/05/revised PY - 2014/02/10/accepted PY - 2014/2/25/entrez PY - 2014/2/25/pubmed PY - 2014/11/15/medline KW - 5,3′,4′-Trihydroxy-6″,6″-dimethylpyrano [2″,3″:7,6] isoflavone KW - Carrageenan KW - Mice KW - Pleurisy KW - Polygala molluginifolia KW - Rutin SP - 334 EP - 41 JF - International immunopharmacology JO - Int. Immunopharmacol. VL - 19 IS - 2 N2 - UNLABELLED: This study was conducted to investigate the anti-inflammatory activity of Polygala molluginifolia (Polygalaceae) on the mouse pleurisy model induced by carrageenan. P. molluginifolia is a plant native to southern Brazil that is popularly called "canfora". The Polygala genus is used to treat different pathologies, including inflammatory diseases, in traditional medicine. MATERIAL AND METHODS: The whole P. molluginifolia plant material was extracted by maceration with 96% ethanol. The crude hydroalcoholic extract (CE) was subjected to chromatographic procedures to produce various derivate fractions, including its aqueous (Aq), ethyl acetate (EtOAc), and hexane (Hex) fractions. Compound 1 (5,3',4'-trihydroxy-6″,6″-dimethylpyrano [2″,3″:7,6] isoflavone) (Iso), which was isolated from the EtOAc fraction, and Compound 2 (rutin) (Rut), which was isolated from the Aq fraction, were identified using ¹H and ¹³C NMR spectroscopy and quantified using an HPLC apparatus. RESULTS: The CE, the Aq, EtOAc, and Hex fractions, and the isolated compounds Iso and Rut were able to reduce cell migration and exudation. Furthermore, the plant material also decreased the myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities and the nitric oxide (NO(x)), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) levels. In addition, Iso and Rut reduced the TNF-α and IL-1β mRNA expression levels and significantly decreased NF-κB p65 phosphorylation. CONCLUSION: The results show that P. molluginifolia has a significant anti-inflammatory action and that this effect is due, at least in part, to the presence of Iso and Rut in large amounts. Moreover, this effect was found to be closely related to the inhibitory effects of the isolated compounds on the NF-κB pathway. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/24560858/Polygala_molluginifolia_A__St__Hil__and_Moq__prevent_inflammation_in_the_mouse_pleurisy_model_by_inhibiting_NF_κB_activation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(14)00064-2 DB - PRIME DP - Unbound Medicine ER -