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Histamine release from human basophils by synthetic block co-polymers composed of polyoxyethylene and polyoxypropylene and synergy with immunologic and non-immunologic stimuli.
J Immunol. 1988 Aug 15; 141(4):1307-10.JI

Abstract

Co-polymers composed of polyoxyethylene and polyoxypropylene have been shown previously to trigger histamine release from mouse peritoneal mast cells; this property quantitatively is directly related to the ionophorous ability of these compounds to cause a functional exchange of intracellular K+ for extracellular Na+ across the cell membrane. We investigated the effect of an inflammatory copolymer, T130R2, on human basophils. The data demonstrate that T130R2 can cause calcium-dependent histamine release from human basophils in vitro. Further, at concentrations that do not cause histamine release, this co-polymer markedly augments release by suboptimal concentrations of the lectin Con A or anti-IgE antibody and the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate but not the calcium ionophore A23187. Thus, these co-polymers induce mediator release from cells of both rodents and humans. In both instances it is likely that calcium-dependent cell triggering is the result of an influx of sodium ions with concomitant depolarization of the transmembrane potential. In common with the calcium ionophore A23187, the co-polymer T130R2 has the ability to synergize with stimuli which trigger the IgE receptor as well as those which directly activate the cellular calcium- and phospholipid-dependent protein kinase.

Authors+Show Affiliations

Atkinson TP
Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322.
Smith TF
No affiliation info available
Hunter RL
No affiliation info available

MeSH

Adjuvants, ImmunologicAdultAntibodies, Anti-IdiotypicBasophilsCalcimycinCalciumConcanavalin ADose-Response Relationship, ImmunologicDrug SynergismHistamine ReleaseHumansImmunoglobulin EPoloxalenePolyethylene GlycolsTetradecanoylphorbol Acetate

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2456350

Citation

Atkinson, T P., et al. "Histamine Release From Human Basophils By Synthetic Block Co-polymers Composed of Polyoxyethylene and Polyoxypropylene and Synergy With Immunologic and Non-immunologic Stimuli." Journal of Immunology (Baltimore, Md. : 1950), vol. 141, no. 4, 1988, pp. 1307-10.
Atkinson TP, Smith TF, Hunter RL. Histamine release from human basophils by synthetic block co-polymers composed of polyoxyethylene and polyoxypropylene and synergy with immunologic and non-immunologic stimuli. J Immunol. 1988;141(4):1307-10.
Atkinson, T. P., Smith, T. F., & Hunter, R. L. (1988). Histamine release from human basophils by synthetic block co-polymers composed of polyoxyethylene and polyoxypropylene and synergy with immunologic and non-immunologic stimuli. Journal of Immunology (Baltimore, Md. : 1950), 141(4), 1307-10.
Atkinson TP, Smith TF, Hunter RL. Histamine Release From Human Basophils By Synthetic Block Co-polymers Composed of Polyoxyethylene and Polyoxypropylene and Synergy With Immunologic and Non-immunologic Stimuli. J Immunol. 1988 Aug 15;141(4):1307-10. PubMed PMID: 2456350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Histamine release from human basophils by synthetic block co-polymers composed of polyoxyethylene and polyoxypropylene and synergy with immunologic and non-immunologic stimuli. AU - Atkinson,T P, AU - Smith,T F, AU - Hunter,R L, PY - 1988/8/15/pubmed PY - 1988/8/15/medline PY - 1988/8/15/entrez SP - 1307 EP - 10 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 141 IS - 4 N2 - Co-polymers composed of polyoxyethylene and polyoxypropylene have been shown previously to trigger histamine release from mouse peritoneal mast cells; this property quantitatively is directly related to the ionophorous ability of these compounds to cause a functional exchange of intracellular K+ for extracellular Na+ across the cell membrane. We investigated the effect of an inflammatory copolymer, T130R2, on human basophils. The data demonstrate that T130R2 can cause calcium-dependent histamine release from human basophils in vitro. Further, at concentrations that do not cause histamine release, this co-polymer markedly augments release by suboptimal concentrations of the lectin Con A or anti-IgE antibody and the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate but not the calcium ionophore A23187. Thus, these co-polymers induce mediator release from cells of both rodents and humans. In both instances it is likely that calcium-dependent cell triggering is the result of an influx of sodium ions with concomitant depolarization of the transmembrane potential. In common with the calcium ionophore A23187, the co-polymer T130R2 has the ability to synergize with stimuli which trigger the IgE receptor as well as those which directly activate the cellular calcium- and phospholipid-dependent protein kinase. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2456350/Histamine_release_from_human_basophils_by_synthetic_block_co_polymers_composed_of_polyoxyethylene_and_polyoxypropylene_and_synergy_with_immunologic_and_non_immunologic_stimuli_ DB - PRIME DP - Unbound Medicine ER -