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Abnormal mitosis induced by wheat-rye 1R monosomic addition lines.
Genome. 2014 Jan; 57(1):21-8.G

Abstract

Octoploid triticale were derived from common wheat (Triticum aestivum L. 'Mianyang11') × rye (Secale cereale L. 'Kustro'), and some progeny were obtained by the backcrossing of triticale with 'Mianyang11' followed by self-fertilization. In situ hybridization using rye genomic DNA and repetitive sequences pAs1 and pSc119.2 as probes was used to analyze the mitotic chromosomes of these progeny. Three wheat-rye 1R monosomic addition lines and a wheat line (12FT-1685) containing a 1R and a 1BL.1RS translocation chromosome were identified. Abnormal mitosis was observed in the two lines. During mitosis of a 1R monosomic addition line (3-8-20-1R-2), lagging chromosomes, micronuclei, chromosomal bridges, and the one pole segregation of 1R chromosome were observed. Abnormal mitotic behaviour of chromosomes was also observed in some of the self-progeny plants of lines 12FT-1685 and 3-8-20-1R-2. These progeny contained 1R chromosome or 1R chromosome arm. In addition, 4B chromosomes were absent from one of the progeny of 3-8-20-1R-2. This abnormal mitotic behaviour of chromosomes was not observed in two other 1R monosomic addition lines. These results indicate that a single 1R chromosome added to wheat might cause abnormal mitotic behaviour of both wheat and rye chromosomes and different genetic variations might occurr among the sibling 1R monosomic addition lines.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Fu SL
State Key Laboratory of Plant Breeding and Genetics, Sichuan Agriculture University, Wenjiang, Chengdu, Sichuan 611130, China.
Yang MY
No affiliation info available
Ren ZL
No affiliation info available
Yan BJ
No affiliation info available
Tang ZX
No affiliation info available

MeSH

Chromosome AberrationsChromosomes, PlantGenetic VariationHybridization, GeneticIn Situ Hybridization, FluorescenceMitosisMonosomySecaleTranslocation, GeneticTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24564212

Citation

Fu, Shu-Lan, et al. "Abnormal Mitosis Induced By Wheat-rye 1R Monosomic Addition Lines." Genome, vol. 57, no. 1, 2014, pp. 21-8.
Fu SL, Yang MY, Ren ZL, et al. Abnormal mitosis induced by wheat-rye 1R monosomic addition lines. Genome. 2014;57(1):21-8.
Fu, S. L., Yang, M. Y., Ren, Z. L., Yan, B. J., & Tang, Z. X. (2014). Abnormal mitosis induced by wheat-rye 1R monosomic addition lines. Genome, 57(1), 21-8. https://doi.org/10.1139/gen-2013-0115
Fu SL, et al. Abnormal Mitosis Induced By Wheat-rye 1R Monosomic Addition Lines. Genome. 2014;57(1):21-8. PubMed PMID: 24564212.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Abnormal mitosis induced by wheat-rye 1R monosomic addition lines. AU - Fu,Shu-Lan, AU - Yang,Man-Yu, AU - Ren,Zheng-Long, AU - Yan,Ben-Ju, AU - Tang,Zong-Xiang, Y1 - 2013/12/17/ PY - 2014/2/26/entrez PY - 2014/2/26/pubmed PY - 2014/10/30/medline SP - 21 EP - 8 JF - Genome JO - Genome VL - 57 IS - 1 N2 - Octoploid triticale were derived from common wheat (Triticum aestivum L. 'Mianyang11') × rye (Secale cereale L. 'Kustro'), and some progeny were obtained by the backcrossing of triticale with 'Mianyang11' followed by self-fertilization. In situ hybridization using rye genomic DNA and repetitive sequences pAs1 and pSc119.2 as probes was used to analyze the mitotic chromosomes of these progeny. Three wheat-rye 1R monosomic addition lines and a wheat line (12FT-1685) containing a 1R and a 1BL.1RS translocation chromosome were identified. Abnormal mitosis was observed in the two lines. During mitosis of a 1R monosomic addition line (3-8-20-1R-2), lagging chromosomes, micronuclei, chromosomal bridges, and the one pole segregation of 1R chromosome were observed. Abnormal mitotic behaviour of chromosomes was also observed in some of the self-progeny plants of lines 12FT-1685 and 3-8-20-1R-2. These progeny contained 1R chromosome or 1R chromosome arm. In addition, 4B chromosomes were absent from one of the progeny of 3-8-20-1R-2. This abnormal mitotic behaviour of chromosomes was not observed in two other 1R monosomic addition lines. These results indicate that a single 1R chromosome added to wheat might cause abnormal mitotic behaviour of both wheat and rye chromosomes and different genetic variations might occurr among the sibling 1R monosomic addition lines. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/24564212/Abnormal_mitosis_induced_by_wheat_rye_1R_monosomic_addition_lines_ DB - PRIME DP - Unbound Medicine ER -