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Isolation and characterization of a wheat--Psathyrostachys huashanica 'Keng' 3Ns disomic addition line with resistance to stripe rust.
Genome. 2014 Jan; 57(1):37-44.G

Abstract

We isolated a wheat germplasm line, 22-2, which was derived from common wheat (Triticum aestivum '7182') and Psathyrostachys huashanica 'Keng' (2n = 2x = 14, NsNs). Genomic composition and homoeologous relationships of 22-2 was analyzed using cytology, genomic in situ hybridization (GISH), EST-SSR, and EST-STS to characterize the alien chromatin in the transfer line. The cytological investigations showed that the chromosome number and configuration were 2n = 44 = 22 II. Mitotic and meiotic GISH using P. huashanica genomic DNA as the probe indicated that 22-2 contained a pair of P. huashanica chromosomes. The genomic affinities of the introduced P. huashanica chromosomes were determined by EST-SSR and EST-STS using multiple-loci markers from seven wheat homoeologous groups between the parents and addition line. One EST-SSR and 17 EST-STS markers, which were located on the homoeologous group 3 chromosomes of wheat, amplified polymorphic bands in 22-2 that were unique to P. huashanica. Thus, these markers suggested that the introduced Ns chromosome pair belonged to homoeologous group 3, so we designated 22-2 as a 3Ns disomic addition line. Based on disease reaction to mixed races (CYR31, CYR32, and Shuiyuan14) of stripe rust in the adult stages, 22-2 was found to have high resistance to stripe rust, which was possibly derived from its P. huashanica parent. Consequently, the new disomic addition line 22-2 could be a valuable donor source for wheat improvement depending on the excellent agronomic traits, especially, the introduction of novel disease resistance genes into wheat during breeding programs.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Du W
Shaanxi Key Laboratory of Genetic Engineering for Plant Breeding, College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.
Wang J
No affiliation info available
Pang Y
No affiliation info available
Wang L
No affiliation info available
Wu J
No affiliation info available
Zhao J
No affiliation info available
Yang Q
No affiliation info available
Chen X
No affiliation info available

MeSH

BasidiomycotaChromatinChromosome PairingChromosomes, PlantCytogenetic AnalysisDisease ResistanceGenetic MarkersHybridization, GeneticPlant DiseasesPoaceaeTriticumUniparental Disomy

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24564214

Citation

Du, Wanli, et al. "Isolation and Characterization of a wheat--Psathyrostachys Huashanica 'Keng' 3Ns Disomic Addition Line With Resistance to Stripe Rust." Genome, vol. 57, no. 1, 2014, pp. 37-44.
Du W, Wang J, Pang Y, et al. Isolation and characterization of a wheat--Psathyrostachys huashanica 'Keng' 3Ns disomic addition line with resistance to stripe rust. Genome. 2014;57(1):37-44.
Du, W., Wang, J., Pang, Y., Wang, L., Wu, J., Zhao, J., Yang, Q., & Chen, X. (2014). Isolation and characterization of a wheat--Psathyrostachys huashanica 'Keng' 3Ns disomic addition line with resistance to stripe rust. Genome, 57(1), 37-44. https://doi.org/10.1139/gen-2013-0199
Du W, et al. Isolation and Characterization of a wheat--Psathyrostachys Huashanica 'Keng' 3Ns Disomic Addition Line With Resistance to Stripe Rust. Genome. 2014;57(1):37-44. PubMed PMID: 24564214.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of a wheat--Psathyrostachys huashanica 'Keng' 3Ns disomic addition line with resistance to stripe rust. AU - Du,Wanli, AU - Wang,Jing, AU - Pang,Yuhui, AU - Wang,Liangming, AU - Wu,Jun, AU - Zhao,Jixin, AU - Yang,Qunhui, AU - Chen,Xinhong, Y1 - 2014/01/02/ PY - 2014/2/26/entrez PY - 2014/2/26/pubmed PY - 2014/10/30/medline SP - 37 EP - 44 JF - Genome JO - Genome VL - 57 IS - 1 N2 - We isolated a wheat germplasm line, 22-2, which was derived from common wheat (Triticum aestivum '7182') and Psathyrostachys huashanica 'Keng' (2n = 2x = 14, NsNs). Genomic composition and homoeologous relationships of 22-2 was analyzed using cytology, genomic in situ hybridization (GISH), EST-SSR, and EST-STS to characterize the alien chromatin in the transfer line. The cytological investigations showed that the chromosome number and configuration were 2n = 44 = 22 II. Mitotic and meiotic GISH using P. huashanica genomic DNA as the probe indicated that 22-2 contained a pair of P. huashanica chromosomes. The genomic affinities of the introduced P. huashanica chromosomes were determined by EST-SSR and EST-STS using multiple-loci markers from seven wheat homoeologous groups between the parents and addition line. One EST-SSR and 17 EST-STS markers, which were located on the homoeologous group 3 chromosomes of wheat, amplified polymorphic bands in 22-2 that were unique to P. huashanica. Thus, these markers suggested that the introduced Ns chromosome pair belonged to homoeologous group 3, so we designated 22-2 as a 3Ns disomic addition line. Based on disease reaction to mixed races (CYR31, CYR32, and Shuiyuan14) of stripe rust in the adult stages, 22-2 was found to have high resistance to stripe rust, which was possibly derived from its P. huashanica parent. Consequently, the new disomic addition line 22-2 could be a valuable donor source for wheat improvement depending on the excellent agronomic traits, especially, the introduction of novel disease resistance genes into wheat during breeding programs. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/24564214/Isolation_and_characterization_of_a_wheat__Psathyrostachys_huashanica_'Keng'_3Ns_disomic_addition_line_with_resistance_to_stripe_rust_ DB - PRIME DP - Unbound Medicine ER -