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Combined administration of curcumin and gallic acid inhibits gallic acid-induced suppression of steroidogenesis, sperm output, antioxidant defenses and inflammatory responsive genes.
J Steroid Biochem Mol Biol. 2014 Sep; 143:49-60.JS

Abstract

In this study, we investigated the effects of administration of gallic acid (Gal) with or without curcumin (Cur) on the sperm output, steroid level and antioxidant defenses in rat testis in vivo and the expression of inflammatory responsive genes in vitro. Male Wistar rats were divided randomly into four groups and given oral Gal (100mg/kg/day) and Cur (100mg/kg/day) alone or in combination for four weeks. The sperm quality was impaired following Gal treatment, while Cur prevented this and also improved the sperm count as well as the efficiency of sperm production (DSP/gm testis). The inhibitory effects of Gal on plasma testosterone level, glutathione levels, activities of glutathione peroxidase, catalase, superoxide dismutase and steroidogenic enzymes, 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-HSD in the rat testis was blocked by Cur. Interestingly, the level of testosterone and the activities of the steroidogenic enzymes were significantly increased after treatment with Cur alone. Malondialdehyde concentration was unchanged following Gal treatment, while a significant decrease in malondialdehyde level was observed following treatment with Cur alone or in combination with Gal. We further analyzed the effects of Cur and Gal (25-100 μM) on the 93RS2 Sertoli cell-lines and observed that Cur blocked the Gal-induced suppression of inflammatory mediators such as TNF-α and IL-6, while Gal blocked the suppressive effect of Cur on IL-1α expression. Furthermore, the stimulatory or inhibitory effects of Gal on the expressions Tgf-β1 and CD-14 was concentration-dependent and could be blocked by Cur. When cultures of primary Sertoli cells were exposed to both Cur and Gal for 24h, p-JNK/SAPK expression remain stable, whereas Gal-induced p-p65 (NF-κB) expression and IκBα degradation was seen to be blocked by Cur but not Gal-induced expression of pERK1/2. Overall, Cur has stimulatory reproductive effects and could protect the testis from the toxic effects of Gal by mechanisms that could not be explained by its effects on the expressions of inflammatory cytokines but by its anti-oxidant properties.

Authors+Show Affiliations

Department of Chemical Sciences, Redeemer's University, Redemption Camp, Ogun State, Nigeria. Electronic address: abarikwus@gmail.com.Department of Chemical Sciences, Redeemer's University, Redemption Camp, Ogun State, Nigeria.Department of Chemical Sciences, Redeemer's University, Redemption Camp, Ogun State, Nigeria.Department of Chemical Sciences, Redeemer's University, Redemption Camp, Ogun State, Nigeria.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24565563

Citation

Abarikwu, Sunny O., et al. "Combined Administration of Curcumin and Gallic Acid Inhibits Gallic Acid-induced Suppression of Steroidogenesis, Sperm Output, Antioxidant Defenses and Inflammatory Responsive Genes." The Journal of Steroid Biochemistry and Molecular Biology, vol. 143, 2014, pp. 49-60.
Abarikwu SO, Akiri OF, Durojaiye MA, et al. Combined administration of curcumin and gallic acid inhibits gallic acid-induced suppression of steroidogenesis, sperm output, antioxidant defenses and inflammatory responsive genes. J Steroid Biochem Mol Biol. 2014;143:49-60.
Abarikwu, S. O., Akiri, O. F., Durojaiye, M. A., & Alabi, A. F. (2014). Combined administration of curcumin and gallic acid inhibits gallic acid-induced suppression of steroidogenesis, sperm output, antioxidant defenses and inflammatory responsive genes. The Journal of Steroid Biochemistry and Molecular Biology, 143, 49-60. https://doi.org/10.1016/j.jsbmb.2014.02.008
Abarikwu SO, et al. Combined Administration of Curcumin and Gallic Acid Inhibits Gallic Acid-induced Suppression of Steroidogenesis, Sperm Output, Antioxidant Defenses and Inflammatory Responsive Genes. J Steroid Biochem Mol Biol. 2014;143:49-60. PubMed PMID: 24565563.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined administration of curcumin and gallic acid inhibits gallic acid-induced suppression of steroidogenesis, sperm output, antioxidant defenses and inflammatory responsive genes. AU - Abarikwu,Sunny O, AU - Akiri,Oghenetega F, AU - Durojaiye,Mojisola A, AU - Alabi,Adenike F, Y1 - 2014/02/22/ PY - 2013/10/01/received PY - 2014/01/24/revised PY - 2014/02/13/accepted PY - 2014/2/26/entrez PY - 2014/2/26/pubmed PY - 2014/12/30/medline KW - Curcumin KW - Gallic acid KW - Inflammatory cytokines KW - Sertoli cells KW - Sperm quality KW - Testis KW - Testosterone SP - 49 EP - 60 JF - The Journal of steroid biochemistry and molecular biology JO - J Steroid Biochem Mol Biol VL - 143 N2 - In this study, we investigated the effects of administration of gallic acid (Gal) with or without curcumin (Cur) on the sperm output, steroid level and antioxidant defenses in rat testis in vivo and the expression of inflammatory responsive genes in vitro. Male Wistar rats were divided randomly into four groups and given oral Gal (100mg/kg/day) and Cur (100mg/kg/day) alone or in combination for four weeks. The sperm quality was impaired following Gal treatment, while Cur prevented this and also improved the sperm count as well as the efficiency of sperm production (DSP/gm testis). The inhibitory effects of Gal on plasma testosterone level, glutathione levels, activities of glutathione peroxidase, catalase, superoxide dismutase and steroidogenic enzymes, 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-HSD in the rat testis was blocked by Cur. Interestingly, the level of testosterone and the activities of the steroidogenic enzymes were significantly increased after treatment with Cur alone. Malondialdehyde concentration was unchanged following Gal treatment, while a significant decrease in malondialdehyde level was observed following treatment with Cur alone or in combination with Gal. We further analyzed the effects of Cur and Gal (25-100 μM) on the 93RS2 Sertoli cell-lines and observed that Cur blocked the Gal-induced suppression of inflammatory mediators such as TNF-α and IL-6, while Gal blocked the suppressive effect of Cur on IL-1α expression. Furthermore, the stimulatory or inhibitory effects of Gal on the expressions Tgf-β1 and CD-14 was concentration-dependent and could be blocked by Cur. When cultures of primary Sertoli cells were exposed to both Cur and Gal for 24h, p-JNK/SAPK expression remain stable, whereas Gal-induced p-p65 (NF-κB) expression and IκBα degradation was seen to be blocked by Cur but not Gal-induced expression of pERK1/2. Overall, Cur has stimulatory reproductive effects and could protect the testis from the toxic effects of Gal by mechanisms that could not be explained by its effects on the expressions of inflammatory cytokines but by its anti-oxidant properties. SN - 1879-1220 UR - https://www.unboundmedicine.com/medline/citation/24565563/Combined_administration_of_curcumin_and_gallic_acid_inhibits_gallic_acid_induced_suppression_of_steroidogenesis_sperm_output_antioxidant_defenses_and_inflammatory_responsive_genes_ DB - PRIME DP - Unbound Medicine ER -