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Dietary supplementation of aspartate enhances intestinal integrity and energy status in weanling piglets after lipopolysaccharide challenge.
J Nutr Biochem. 2014 Apr; 25(4):456-62.JN

Abstract

The intestine has a high requirement for ATP to support its integrity, function and health, and thus, energy deficits in the intestinal mucosa may play a critical role in intestinal injury. Aspartate (Asp) is one of the major sources of ATP in mammalian enterocytes via mitochondrial oxidation. We hypothesized that dietary supplementation of Asp could attenuate lipopolysaccharide (LPS)-induced intestinal damage via modulation of intestinal energy status. Twenty-four weanling piglets were allotted to one of four treatments: (1) nonchallenged control, (2) LPS-challenged control, (3) LPS+0.5% Asp treatment, and (4) LPS+1.0% Asp treatment. On day 19, pigs were injected with saline or LPS. At 24 h postinjection, pigs were killed and intestinal samples were obtained. Asp attenuated LPS-induced intestinal damage indicated by greater villus height and villus height/crypt depth ratio as well as higher RNA/DNA and protein/DNA ratios. Asp improved intestinal function indicated by increased intestinal mucosal disaccharidase activities. Asp also improved intestinal energy status indicated by increased ATP, ADP and total adenine nucleotide contents, adenylate energy charge and decreased AMP/ATP ratio. In addition, Asp increased the activities of tricarboxylic acid cycle key enzymes including citrate synthase, isocitrate dehydrogenase and alpha-oxoglutarate dehydrogenase complex. Moreover, Asp down-regulated mRNA expression of intestinal AMP-activated protein kinase α1 (AMPKα1), AMPKα2, silent information regulator 1 (SIRT1) and peroxisome proliferator-activated receptor gamma coactivator-1α (PGC1α) and decreased intestinal AMPKα phosphorylation. These results indicate that Asp may alleviate LPS-induced intestinal damage and improve intestinal energy status.

Authors+Show Affiliations

Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: yulanflower@126.com.Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Laboratory of Developmental Nutrition, Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.Laboratory of Developmental Nutrition, Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24565675

Citation

Pi, Dingan, et al. "Dietary Supplementation of Aspartate Enhances Intestinal Integrity and Energy Status in Weanling Piglets After Lipopolysaccharide Challenge." The Journal of Nutritional Biochemistry, vol. 25, no. 4, 2014, pp. 456-62.
Pi D, Liu Y, Shi H, et al. Dietary supplementation of aspartate enhances intestinal integrity and energy status in weanling piglets after lipopolysaccharide challenge. J Nutr Biochem. 2014;25(4):456-62.
Pi, D., Liu, Y., Shi, H., Li, S., Odle, J., Lin, X., Zhu, H., Chen, F., Hou, Y., & Leng, W. (2014). Dietary supplementation of aspartate enhances intestinal integrity and energy status in weanling piglets after lipopolysaccharide challenge. The Journal of Nutritional Biochemistry, 25(4), 456-62. https://doi.org/10.1016/j.jnutbio.2013.12.006
Pi D, et al. Dietary Supplementation of Aspartate Enhances Intestinal Integrity and Energy Status in Weanling Piglets After Lipopolysaccharide Challenge. J Nutr Biochem. 2014;25(4):456-62. PubMed PMID: 24565675.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary supplementation of aspartate enhances intestinal integrity and energy status in weanling piglets after lipopolysaccharide challenge. AU - Pi,Dingan, AU - Liu,Yulan, AU - Shi,Haifeng, AU - Li,Shuang, AU - Odle,Jack, AU - Lin,Xi, AU - Zhu,Huiling, AU - Chen,Feng, AU - Hou,Yongqing, AU - Leng,Weibo, Y1 - 2014/01/16/ PY - 2013/09/17/received PY - 2013/12/05/revised PY - 2013/12/18/accepted PY - 2014/2/26/entrez PY - 2014/2/26/pubmed PY - 2015/5/13/medline KW - Aspartate KW - Energy status KW - Intestine KW - Lipopolysaccharide KW - Weanling piglets SP - 456 EP - 62 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 25 IS - 4 N2 - The intestine has a high requirement for ATP to support its integrity, function and health, and thus, energy deficits in the intestinal mucosa may play a critical role in intestinal injury. Aspartate (Asp) is one of the major sources of ATP in mammalian enterocytes via mitochondrial oxidation. We hypothesized that dietary supplementation of Asp could attenuate lipopolysaccharide (LPS)-induced intestinal damage via modulation of intestinal energy status. Twenty-four weanling piglets were allotted to one of four treatments: (1) nonchallenged control, (2) LPS-challenged control, (3) LPS+0.5% Asp treatment, and (4) LPS+1.0% Asp treatment. On day 19, pigs were injected with saline or LPS. At 24 h postinjection, pigs were killed and intestinal samples were obtained. Asp attenuated LPS-induced intestinal damage indicated by greater villus height and villus height/crypt depth ratio as well as higher RNA/DNA and protein/DNA ratios. Asp improved intestinal function indicated by increased intestinal mucosal disaccharidase activities. Asp also improved intestinal energy status indicated by increased ATP, ADP and total adenine nucleotide contents, adenylate energy charge and decreased AMP/ATP ratio. In addition, Asp increased the activities of tricarboxylic acid cycle key enzymes including citrate synthase, isocitrate dehydrogenase and alpha-oxoglutarate dehydrogenase complex. Moreover, Asp down-regulated mRNA expression of intestinal AMP-activated protein kinase α1 (AMPKα1), AMPKα2, silent information regulator 1 (SIRT1) and peroxisome proliferator-activated receptor gamma coactivator-1α (PGC1α) and decreased intestinal AMPKα phosphorylation. These results indicate that Asp may alleviate LPS-induced intestinal damage and improve intestinal energy status. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/24565675/Dietary_supplementation_of_aspartate_enhances_intestinal_integrity_and_energy_status_in_weanling_piglets_after_lipopolysaccharide_challenge_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(14)00003-5 DB - PRIME DP - Unbound Medicine ER -