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The effect of the chain length distribution of free fatty acids on the mixing properties of stratum corneum model membranes.
Biochim Biophys Acta. 2014 Jul; 1838(7):1851-61.BB

Abstract

The stratum corneum (SC) plays a fundamental role in the barrier function of the skin. The SC consists of corneocytes embedded in a lipid matrix. The main lipid classes in the lipid matrix are ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs). The aim of this study was to examine the effect of the chain length of FFAs on the thermotropic phase behavior and mixing properties of SC lipids. Fourier transform infrared spectroscopy and Raman imaging spectroscopy were used to study the mixing properties using either protonated or deuterated FFAs. We selected SC model lipid mixtures containing only a single CER, CHOL and either a single FFA or a mixture of FFAs mimicking the FFA SC composition. The single CER consists of a sphingoid base with 18 carbon atoms and an acyl chain with a chain length of 24 carbon atoms. When using lignoceric acid (24 carbon atoms) or a mixture of FFAs, the CER and FFAs participated in mixed crystals, but hydration of the mixtures induced a slight phase separation between CER and FFA. The mixed crystalline structures did not phase separate during storage even up to a time period of 3months. When using palmitic acid (16 carbon atoms), a slight phase separation was observed between FFA and CER. This phase separation was clearly enhanced during hydration and storage. In conclusion, the thermotropic phase behavior and the mixing properties of the SC lipid mixtures were shown to strongly depend on the chain length and chain length distribution of FFAs, while hydration enhanced the phase separation.

Authors+Show Affiliations

Analytical Science Research Laboratories, Kao Corporation, Akabane 2606, Ichikai, Haga, Tochigi 321-3497, Japan; Leiden/Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Leiden University, Einsteinweg 55, 2333 CC Leiden, the Netherlands.Leiden/Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Leiden University, Einsteinweg 55, 2333 CC Leiden, the Netherlands.Analytical Science Research Laboratories, Kao Corporation, Akabane 2606, Ichikai, Haga, Tochigi 321-3497, Japan.Leiden/Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Leiden University, Einsteinweg 55, 2333 CC Leiden, the Netherlands. Electronic address: bouwstra@lacdr.leidenuniv.nl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24565794

Citation

Oguri, Masashi, et al. "The Effect of the Chain Length Distribution of Free Fatty Acids On the Mixing Properties of Stratum Corneum Model Membranes." Biochimica Et Biophysica Acta, vol. 1838, no. 7, 2014, pp. 1851-61.
Oguri M, Gooris GS, Bito K, et al. The effect of the chain length distribution of free fatty acids on the mixing properties of stratum corneum model membranes. Biochim Biophys Acta. 2014;1838(7):1851-61.
Oguri, M., Gooris, G. S., Bito, K., & Bouwstra, J. A. (2014). The effect of the chain length distribution of free fatty acids on the mixing properties of stratum corneum model membranes. Biochimica Et Biophysica Acta, 1838(7), 1851-61. https://doi.org/10.1016/j.bbamem.2014.02.009
Oguri M, et al. The Effect of the Chain Length Distribution of Free Fatty Acids On the Mixing Properties of Stratum Corneum Model Membranes. Biochim Biophys Acta. 2014;1838(7):1851-61. PubMed PMID: 24565794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of the chain length distribution of free fatty acids on the mixing properties of stratum corneum model membranes. AU - Oguri,Masashi, AU - Gooris,Gert S, AU - Bito,Kotatsu, AU - Bouwstra,Joke A, Y1 - 2014/02/22/ PY - 2013/11/07/received PY - 2014/02/05/revised PY - 2014/02/13/accepted PY - 2014/2/26/entrez PY - 2014/2/26/pubmed PY - 2014/7/25/medline KW - FTIR KW - Kinetics KW - Lipid organization KW - Skin KW - Stratum corneum SP - 1851 EP - 61 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1838 IS - 7 N2 - The stratum corneum (SC) plays a fundamental role in the barrier function of the skin. The SC consists of corneocytes embedded in a lipid matrix. The main lipid classes in the lipid matrix are ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs). The aim of this study was to examine the effect of the chain length of FFAs on the thermotropic phase behavior and mixing properties of SC lipids. Fourier transform infrared spectroscopy and Raman imaging spectroscopy were used to study the mixing properties using either protonated or deuterated FFAs. We selected SC model lipid mixtures containing only a single CER, CHOL and either a single FFA or a mixture of FFAs mimicking the FFA SC composition. The single CER consists of a sphingoid base with 18 carbon atoms and an acyl chain with a chain length of 24 carbon atoms. When using lignoceric acid (24 carbon atoms) or a mixture of FFAs, the CER and FFAs participated in mixed crystals, but hydration of the mixtures induced a slight phase separation between CER and FFA. The mixed crystalline structures did not phase separate during storage even up to a time period of 3months. When using palmitic acid (16 carbon atoms), a slight phase separation was observed between FFA and CER. This phase separation was clearly enhanced during hydration and storage. In conclusion, the thermotropic phase behavior and the mixing properties of the SC lipid mixtures were shown to strongly depend on the chain length and chain length distribution of FFAs, while hydration enhanced the phase separation. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/24565794/The_effect_of_the_chain_length_distribution_of_free_fatty_acids_on_the_mixing_properties_of_stratum_corneum_model_membranes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0005-2736(14)00073-X DB - PRIME DP - Unbound Medicine ER -