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Influence of aqueous media on the ROS-mediated toxicity of ZnO nanoparticles toward green fluorescent protein-expressing Escherichia coli under UV-365 irradiation.
Langmuir. 2014 Mar 18; 30(10):2852-62.L

Abstract

The aqueous media could affect the physicochemical properties (e.g., surface charge, morphology, and aggregation) of ZnO nanoparticles (nZnO), leading to their different environmental impacts. In this study, the toxicity of nZnO toward the green fluorescent protein-expressing Escherichia coli cells under UV-365 light irradiaiton in various media was assessed, including deionized (DI) water, 0.85% NaCl, phosphate-buffered saline (PBS), minimal Davis medium (MD), and Luria-Bertani medium (LB). The toxicity of nZnO was assessed by the conventional plate count method and the fluorescence intensity method, which consistently demonstrated that the nZnO toxicity was dependent on the medium components that varied the potency of reactive oxygen species (ROS) generation. In DI, NaCl, PBS, and MD medium, nZnO generated three types of ROS (O2(•-), •OH, and (1)O2), whereas in LB medium, nZnO generated O2(•-) and (1)O2. The total concentrations of ROS generated by nZnO in DI, NaCl, PBS, MD, and LB were 265.5 ± 15.9, 153.6 ± 8.6, 144.3 ± 6.9, 123.0 ± 6.0, and 115.6 ± 4.5 μM, respectively. Furthermore, a linear correlation was established between the total concentrations of three types of ROS generated by nZnO and their bacterial mortality rate (R(2) = 0.92) in various media. Since the released Zn(2+) from nZnO under UV irradiation only accounted for less than 10% of the total Zn in all media, the ionic forms of zinc did not significantly contribute to the overall toxicity. This work aims at providing further insight into the medium type influences on the ROS production and the toxicity of nZnO toward the E. coli cells.

Authors+Show Affiliations

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University , Beijing 100875, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24568235

Citation

Li, Yang, et al. "Influence of Aqueous Media On the ROS-mediated Toxicity of ZnO Nanoparticles Toward Green Fluorescent Protein-expressing Escherichia Coli Under UV-365 Irradiation." Langmuir : the ACS Journal of Surfaces and Colloids, vol. 30, no. 10, 2014, pp. 2852-62.
Li Y, Niu J, Zhang W, et al. Influence of aqueous media on the ROS-mediated toxicity of ZnO nanoparticles toward green fluorescent protein-expressing Escherichia coli under UV-365 irradiation. Langmuir. 2014;30(10):2852-62.
Li, Y., Niu, J., Zhang, W., Zhang, L., & Shang, E. (2014). Influence of aqueous media on the ROS-mediated toxicity of ZnO nanoparticles toward green fluorescent protein-expressing Escherichia coli under UV-365 irradiation. Langmuir : the ACS Journal of Surfaces and Colloids, 30(10), 2852-62. https://doi.org/10.1021/la5000028
Li Y, et al. Influence of Aqueous Media On the ROS-mediated Toxicity of ZnO Nanoparticles Toward Green Fluorescent Protein-expressing Escherichia Coli Under UV-365 Irradiation. Langmuir. 2014 Mar 18;30(10):2852-62. PubMed PMID: 24568235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of aqueous media on the ROS-mediated toxicity of ZnO nanoparticles toward green fluorescent protein-expressing Escherichia coli under UV-365 irradiation. AU - Li,Yang, AU - Niu,Junfeng, AU - Zhang,Wen, AU - Zhang,Lilan, AU - Shang,Enxiang, Y1 - 2014/03/07/ PY - 2014/2/27/entrez PY - 2014/2/27/pubmed PY - 2014/11/15/medline SP - 2852 EP - 62 JF - Langmuir : the ACS journal of surfaces and colloids JO - Langmuir VL - 30 IS - 10 N2 - The aqueous media could affect the physicochemical properties (e.g., surface charge, morphology, and aggregation) of ZnO nanoparticles (nZnO), leading to their different environmental impacts. In this study, the toxicity of nZnO toward the green fluorescent protein-expressing Escherichia coli cells under UV-365 light irradiaiton in various media was assessed, including deionized (DI) water, 0.85% NaCl, phosphate-buffered saline (PBS), minimal Davis medium (MD), and Luria-Bertani medium (LB). The toxicity of nZnO was assessed by the conventional plate count method and the fluorescence intensity method, which consistently demonstrated that the nZnO toxicity was dependent on the medium components that varied the potency of reactive oxygen species (ROS) generation. In DI, NaCl, PBS, and MD medium, nZnO generated three types of ROS (O2(•-), •OH, and (1)O2), whereas in LB medium, nZnO generated O2(•-) and (1)O2. The total concentrations of ROS generated by nZnO in DI, NaCl, PBS, MD, and LB were 265.5 ± 15.9, 153.6 ± 8.6, 144.3 ± 6.9, 123.0 ± 6.0, and 115.6 ± 4.5 μM, respectively. Furthermore, a linear correlation was established between the total concentrations of three types of ROS generated by nZnO and their bacterial mortality rate (R(2) = 0.92) in various media. Since the released Zn(2+) from nZnO under UV irradiation only accounted for less than 10% of the total Zn in all media, the ionic forms of zinc did not significantly contribute to the overall toxicity. This work aims at providing further insight into the medium type influences on the ROS production and the toxicity of nZnO toward the E. coli cells. SN - 1520-5827 UR - https://www.unboundmedicine.com/medline/citation/24568235/Influence_of_aqueous_media_on_the_ROS_mediated_toxicity_of_ZnO_nanoparticles_toward_green_fluorescent_protein_expressing_Escherichia_coli_under_UV_365_irradiation_ DB - PRIME DP - Unbound Medicine ER -