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Cytotoxicity of Nigella sativa seed oil and extract against human lung cancer cell line.
Asian Pac J Cancer Prev. 2014; 15(2):983-7.AP

Abstract

Nigella sativa (N sativa), commonly known as black seed, has been used in traditional medicine to treat many diseases. The antioxidant, anti-inflammatory, and antibacterial activities of N sativa extracts are well known. Therefore, the present study was designed to investigate the anticancer activity of seed extract (NSE) and seed oil (NSO) of N sativa against a human lung cancer cell line. Cells were exposed to 0.01 to 1 mg/ml of NSE and NSO for 24 h, then percent cell viability was assessed by 3-(4, 5-dimethylthiazol-2yl)-2, 5-biphenyl tetrazolium bromide (MTT) and neutral red uptake (NRU) assays, and cellular morphology by phase contrast inverted microscopy. The results showed NSE and NSO significantly reduce the cell viability and alter the cellular morphology of A-549 cells in a concentration dependent manner. The percent cell viability was recorded as 75%, 50%, and 26% at 0.25, 0.5, and 1 mg/ml of NSE by MTT assay and 73%, 48%, and 23% at 0.25, 0.5, and 1 mg/ml of NSE by NRU assay. Exposure to NSO concentrations of 0.1 mg/ml and above for 24 h was also found to be cytotoxic. The decrease in cell viability at 0.1, 0.25, 0.5, and 1 mg/ml of NSO was recorded to be 89%, 52%, 41%, and 13% by MTT assay and 85%, 52%, 38%, and 11% by NRU assay, respectively. A-549 cells exposed to 0.25, 0.5 and 1 mg/ml of NSE and NSO lost their typical morphology and appeared smaller in size. The data revealed that the treatment of seed extract (NSE) and seed oil (NSO) of Nigella sativa significantly reduce viability of human lung cancer cells.

Authors+Show Affiliations

Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia E-mail : nidachem@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24568529

Citation

Al-Sheddi, Ebtesam Saad, et al. "Cytotoxicity of Nigella Sativa Seed Oil and Extract Against Human Lung Cancer Cell Line." Asian Pacific Journal of Cancer Prevention : APJCP, vol. 15, no. 2, 2014, pp. 983-7.
Al-Sheddi ES, Farshori NN, Al-Oqail MM, et al. Cytotoxicity of Nigella sativa seed oil and extract against human lung cancer cell line. Asian Pac J Cancer Prev. 2014;15(2):983-7.
Al-Sheddi, E. S., Farshori, N. N., Al-Oqail, M. M., Musarrat, J., Al-Khedhairy, A. A., & Siddiqui, M. A. (2014). Cytotoxicity of Nigella sativa seed oil and extract against human lung cancer cell line. Asian Pacific Journal of Cancer Prevention : APJCP, 15(2), 983-7.
Al-Sheddi ES, et al. Cytotoxicity of Nigella Sativa Seed Oil and Extract Against Human Lung Cancer Cell Line. Asian Pac J Cancer Prev. 2014;15(2):983-7. PubMed PMID: 24568529.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxicity of Nigella sativa seed oil and extract against human lung cancer cell line. AU - Al-Sheddi,Ebtesam Saad, AU - Farshori,Nida Nayyar, AU - Al-Oqail,Mai Mohammad, AU - Musarrat,Javed, AU - Al-Khedhairy,Abdulaziz Ali, AU - Siddiqui,Maqsood Ahmed, PY - 2014/2/27/entrez PY - 2014/2/27/pubmed PY - 2014/11/13/medline SP - 983 EP - 7 JF - Asian Pacific journal of cancer prevention : APJCP JO - Asian Pac J Cancer Prev VL - 15 IS - 2 N2 - Nigella sativa (N sativa), commonly known as black seed, has been used in traditional medicine to treat many diseases. The antioxidant, anti-inflammatory, and antibacterial activities of N sativa extracts are well known. Therefore, the present study was designed to investigate the anticancer activity of seed extract (NSE) and seed oil (NSO) of N sativa against a human lung cancer cell line. Cells were exposed to 0.01 to 1 mg/ml of NSE and NSO for 24 h, then percent cell viability was assessed by 3-(4, 5-dimethylthiazol-2yl)-2, 5-biphenyl tetrazolium bromide (MTT) and neutral red uptake (NRU) assays, and cellular morphology by phase contrast inverted microscopy. The results showed NSE and NSO significantly reduce the cell viability and alter the cellular morphology of A-549 cells in a concentration dependent manner. The percent cell viability was recorded as 75%, 50%, and 26% at 0.25, 0.5, and 1 mg/ml of NSE by MTT assay and 73%, 48%, and 23% at 0.25, 0.5, and 1 mg/ml of NSE by NRU assay. Exposure to NSO concentrations of 0.1 mg/ml and above for 24 h was also found to be cytotoxic. The decrease in cell viability at 0.1, 0.25, 0.5, and 1 mg/ml of NSO was recorded to be 89%, 52%, 41%, and 13% by MTT assay and 85%, 52%, 38%, and 11% by NRU assay, respectively. A-549 cells exposed to 0.25, 0.5 and 1 mg/ml of NSE and NSO lost their typical morphology and appeared smaller in size. The data revealed that the treatment of seed extract (NSE) and seed oil (NSO) of Nigella sativa significantly reduce viability of human lung cancer cells. SN - 2476-762X UR - https://www.unboundmedicine.com/medline/citation/24568529/Cytotoxicity_of_Nigella_sativa_seed_oil_and_extract_against_human_lung_cancer_cell_line_ L2 - http://journal.waocp.org/?sid=Entrez:PubMed&id=pmid:24568529&key=2014.15.2.983 DB - PRIME DP - Unbound Medicine ER -