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The effect of nutritional status and muscle fiber type on myogenic satellite cell fate and apoptosis.
Poult Sci. 2014 Jan; 93(1):163-73.PS

Abstract

Satellite cells (SC) are multipotential stem cells that can be induced by nutrition to alter their cellular developmental fate, which may vary depending on their fiber type origin. The objective of the current study was to determine the effect of restricting protein synthesis on inducing adipogenic transdifferentiation and apoptosis of SC originating from fibers of the fast glycolytic pectoralis major (p. major) and fast oxidative and glycolytic biceps femoris (b. femoris) muscles of the chicken. The availability of the essential sulfur amino acids Met and Cys was restricted to regulate protein synthesis during SC proliferation and differentiation. The SC were cultured and treated with 1 of 6 Met/Cys concentrations: 60/192, 30/96 (control), 7.5/24, 3/9.6, 1/3.2, or 0/0 mg/L. Reductions in Met/Cys concentrations from the control level resulted in increased lipid staining and expression of the adipogenic marker genes peroxisome proliferator-activated receptor gamma and stearoyl-CoA desaturase during differentiation in the p. major SC. Although b. femoris SC had increased lipid staining at lower Met/Cys concentrations, there was no increase in expression of either adipogenic gene. For both muscle types, SC Met/Cys, concentration above the control increased the expression of peroxisome proliferator-activated receptor gamma and stearoyl-CoA desaturase during differentiation. As Met/Cys concentration was decreased during proliferation, a dose-dependent decline in all apoptotic cells occurred except for early apoptotic cells in the p. major, which had no treatment effect (P < 0.05). During differentiation, decreasing Met/Cys concentration caused an increase in early apoptotic cells in both fiber types and no effect on late apoptotic cells except for an increase in the p. major 7.5/24 mg/L of Met/Cys treatment. In general, the viability of the SC was unaffected by the Met/Cys concentration except during proliferation in the p. major 0/0 mg/L of Met/Cys treatment, which increased SC viability. These data demonstrate the effect of nutrition on SC transdifferentiation to an adipogenic lineage and apoptosis, and the effect of fiber type on this response in an in vitro context.

Authors+Show Affiliations

Faculty of Veterinary Science, The University of Sydney, Camden, NSW 2750, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24570436

Citation

Powell, D J., et al. "The Effect of Nutritional Status and Muscle Fiber Type On Myogenic Satellite Cell Fate and Apoptosis." Poultry Science, vol. 93, no. 1, 2014, pp. 163-73.
Powell DJ, McFarland DC, Cowieson AJ, et al. The effect of nutritional status and muscle fiber type on myogenic satellite cell fate and apoptosis. Poult Sci. 2014;93(1):163-73.
Powell, D. J., McFarland, D. C., Cowieson, A. J., Muir, W. I., & Velleman, S. G. (2014). The effect of nutritional status and muscle fiber type on myogenic satellite cell fate and apoptosis. Poultry Science, 93(1), 163-73. https://doi.org/10.3382/ps.2013-03450
Powell DJ, et al. The Effect of Nutritional Status and Muscle Fiber Type On Myogenic Satellite Cell Fate and Apoptosis. Poult Sci. 2014;93(1):163-73. PubMed PMID: 24570436.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of nutritional status and muscle fiber type on myogenic satellite cell fate and apoptosis. AU - Powell,D J, AU - McFarland,D C, AU - Cowieson,A J, AU - Muir,W I, AU - Velleman,S G, PY - 2014/2/27/entrez PY - 2014/2/27/pubmed PY - 2014/4/16/medline KW - adipogenesis KW - apoptosis KW - muscle KW - nutrition KW - satellite cell SP - 163 EP - 73 JF - Poultry science JO - Poult Sci VL - 93 IS - 1 N2 - Satellite cells (SC) are multipotential stem cells that can be induced by nutrition to alter their cellular developmental fate, which may vary depending on their fiber type origin. The objective of the current study was to determine the effect of restricting protein synthesis on inducing adipogenic transdifferentiation and apoptosis of SC originating from fibers of the fast glycolytic pectoralis major (p. major) and fast oxidative and glycolytic biceps femoris (b. femoris) muscles of the chicken. The availability of the essential sulfur amino acids Met and Cys was restricted to regulate protein synthesis during SC proliferation and differentiation. The SC were cultured and treated with 1 of 6 Met/Cys concentrations: 60/192, 30/96 (control), 7.5/24, 3/9.6, 1/3.2, or 0/0 mg/L. Reductions in Met/Cys concentrations from the control level resulted in increased lipid staining and expression of the adipogenic marker genes peroxisome proliferator-activated receptor gamma and stearoyl-CoA desaturase during differentiation in the p. major SC. Although b. femoris SC had increased lipid staining at lower Met/Cys concentrations, there was no increase in expression of either adipogenic gene. For both muscle types, SC Met/Cys, concentration above the control increased the expression of peroxisome proliferator-activated receptor gamma and stearoyl-CoA desaturase during differentiation. As Met/Cys concentration was decreased during proliferation, a dose-dependent decline in all apoptotic cells occurred except for early apoptotic cells in the p. major, which had no treatment effect (P < 0.05). During differentiation, decreasing Met/Cys concentration caused an increase in early apoptotic cells in both fiber types and no effect on late apoptotic cells except for an increase in the p. major 7.5/24 mg/L of Met/Cys treatment. In general, the viability of the SC was unaffected by the Met/Cys concentration except during proliferation in the p. major 0/0 mg/L of Met/Cys treatment, which increased SC viability. These data demonstrate the effect of nutrition on SC transdifferentiation to an adipogenic lineage and apoptosis, and the effect of fiber type on this response in an in vitro context. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/24570436/The_effect_of_nutritional_status_and_muscle_fiber_type_on_myogenic_satellite_cell_fate_and_apoptosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)35995-4 DB - PRIME DP - Unbound Medicine ER -