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The effect of nutritional status and myogenic satellite cell age on turkey satellite cell proliferation, differentiation, and expression of myogenic transcriptional regulatory factors and heparan sulfate proteoglycans syndecan-4 and glypican-1.
Poult Sci. 2014 Jan; 93(1):174-86.PS

Abstract

Posthatch satellite cell mitotic activity is a critical component of muscle development and growth. Satellite cells are myogenic stem cells that can be induced by nutrition to follow other cellular developmental pathways, and whose mitotic activity declines with age. The objective of the current study was to determine the effect of restricting protein synthesis on the proliferation and differentiation, expression of myogenic transcriptional regulatory factors myogenic determination factor 1, myogenin, and myogenic regulatory factor 4, and expression of the heparan sulfate proteoglycans syndecan-4 and glypican-1 in satellite cells isolated from 1-d-, 7-wk-, and 16-wk-old turkey pectoralis major muscle (1 d, 7 wk, and 16 wk cells, respectively) by using variable concentrations of Met and Cys. Four Met concentrations-30 (control), 7.5, 3, or 0 mg/L with 3.2 mg/L of Cys per 1 mg/L of Met-were used for culture of satellite cells to determine the effect of nutrition and age on satellite cell behavior during proliferation and differentiation. Proliferation was reduced by lower Met and Cys concentrations in all ages at 96 h of proliferation. Differentiation was increased in the 1 d Met-restricted cells, whereas the 7 wk cells treated with 3 mg/L of Met had decreased differentiation. Reduced Met and Cys levels from the control did not significantly affect the 16 wk cells at 72 h of differentiation. However, medium with no Met or Cys suppressed differentiation at all ages. The expression of myogenic determination factor 1, myogenin, myogenic regulatory factor 4, syndecan-4, and glypican-1 was differentially affected by age and Met or Cys treatment. These data demonstrate the age-specific manner in which turkey pectoralis major muscle satellite cells respond to nutritional availability and the importance of defining optimal nutrition to maximize satellite cell proliferation and differentiation for subsequent muscle mass accretion.

Authors+Show Affiliations

Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster 44691.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24570437

Citation

Harthan, Laura B., et al. "The Effect of Nutritional Status and Myogenic Satellite Cell Age On Turkey Satellite Cell Proliferation, Differentiation, and Expression of Myogenic Transcriptional Regulatory Factors and Heparan Sulfate Proteoglycans Syndecan-4 and Glypican-1." Poultry Science, vol. 93, no. 1, 2014, pp. 174-86.
Harthan LB, McFarland DC, Velleman SG. The effect of nutritional status and myogenic satellite cell age on turkey satellite cell proliferation, differentiation, and expression of myogenic transcriptional regulatory factors and heparan sulfate proteoglycans syndecan-4 and glypican-1. Poult Sci. 2014;93(1):174-86.
Harthan, L. B., McFarland, D. C., & Velleman, S. G. (2014). The effect of nutritional status and myogenic satellite cell age on turkey satellite cell proliferation, differentiation, and expression of myogenic transcriptional regulatory factors and heparan sulfate proteoglycans syndecan-4 and glypican-1. Poultry Science, 93(1), 174-86. https://doi.org/10.3382/ps.2013-03570
Harthan LB, McFarland DC, Velleman SG. The Effect of Nutritional Status and Myogenic Satellite Cell Age On Turkey Satellite Cell Proliferation, Differentiation, and Expression of Myogenic Transcriptional Regulatory Factors and Heparan Sulfate Proteoglycans Syndecan-4 and Glypican-1. Poult Sci. 2014;93(1):174-86. PubMed PMID: 24570437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of nutritional status and myogenic satellite cell age on turkey satellite cell proliferation, differentiation, and expression of myogenic transcriptional regulatory factors and heparan sulfate proteoglycans syndecan-4 and glypican-1. AU - Harthan,Laura B, AU - McFarland,Douglas C, AU - Velleman,Sandra G, PY - 2014/2/27/entrez PY - 2014/2/27/pubmed PY - 2014/4/16/medline KW - heparan sulfate proteoglycan KW - muscle KW - myogenic regulatory factor KW - nutrition KW - satellite cell SP - 174 EP - 86 JF - Poultry science JO - Poult Sci VL - 93 IS - 1 N2 - Posthatch satellite cell mitotic activity is a critical component of muscle development and growth. Satellite cells are myogenic stem cells that can be induced by nutrition to follow other cellular developmental pathways, and whose mitotic activity declines with age. The objective of the current study was to determine the effect of restricting protein synthesis on the proliferation and differentiation, expression of myogenic transcriptional regulatory factors myogenic determination factor 1, myogenin, and myogenic regulatory factor 4, and expression of the heparan sulfate proteoglycans syndecan-4 and glypican-1 in satellite cells isolated from 1-d-, 7-wk-, and 16-wk-old turkey pectoralis major muscle (1 d, 7 wk, and 16 wk cells, respectively) by using variable concentrations of Met and Cys. Four Met concentrations-30 (control), 7.5, 3, or 0 mg/L with 3.2 mg/L of Cys per 1 mg/L of Met-were used for culture of satellite cells to determine the effect of nutrition and age on satellite cell behavior during proliferation and differentiation. Proliferation was reduced by lower Met and Cys concentrations in all ages at 96 h of proliferation. Differentiation was increased in the 1 d Met-restricted cells, whereas the 7 wk cells treated with 3 mg/L of Met had decreased differentiation. Reduced Met and Cys levels from the control did not significantly affect the 16 wk cells at 72 h of differentiation. However, medium with no Met or Cys suppressed differentiation at all ages. The expression of myogenic determination factor 1, myogenin, myogenic regulatory factor 4, syndecan-4, and glypican-1 was differentially affected by age and Met or Cys treatment. These data demonstrate the age-specific manner in which turkey pectoralis major muscle satellite cells respond to nutritional availability and the importance of defining optimal nutrition to maximize satellite cell proliferation and differentiation for subsequent muscle mass accretion. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/24570437/The_effect_of_nutritional_status_and_myogenic_satellite_cell_age_on_turkey_satellite_cell_proliferation_differentiation_and_expression_of_myogenic_transcriptional_regulatory_factors_and_heparan_sulfate_proteoglycans_syndecan_4_and_glypican_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)35996-6 DB - PRIME DP - Unbound Medicine ER -