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Molecular epidemiological survey on quinolone resistance genotype and phenotype of Escherichia coli in septicemic broilers in Hebei, China.
Poult Sci. 2014 Feb; 93(2):335-9.PS

Abstract

In this study, the quinolone-resistant determining region (QRDR) of gyrA of Escherichia coli and plasmid-mediated quinolone resistance (PMQR) genes, qnr(qnrA, qnrB, and qnrS), and aac(6 ')-Ib-cr were detected, sequenced, and analyzed. In addition, antimicrobial susceptibility tests (using the Kirby-Bauer disc diffusion method) were performed for all 111 E. coli isolates from septicemic broilers in Hebei, China. The results show that the resistance rates were as follows: ofloxacin 99.10%, ciprofloxacin 93.69%, levofloxacin 91.89%, norfloxacin 90.09%, and gatifloxacin 76.58%. Of the PMQR genes examined, aac(6 ')-Ib-cr (36.04%) was the most frequently identified gene in all isolates, followed by qnrS (8.11%), qnrB (0.90%), and qnrA (0%). Of the QRDR examined in the 40 phenotypic quinolone-resistant isolates, compared with the gyrA(+) gene of E. coli K-12, 4 amino acid exchanges were found, namely Ser-83→Asp, Asp-87→Asn, Asp-87→Tyr, and Asp-87→Ala, and all 40 isolates had 1 or 2 exchanges in QRDR. It was concluded that quinolone-resistance in E. coli remains a serious problem in Hebei, China. Therefore, there is considerable local surveillance of quinolone resistance. Plasmid-mediated quinolone resistance of the qnr type remains rare in Hebei, China, and mutation in QRDR may be the main problem.

Authors+Show Affiliations

College of Veterinary Medicine, Hebei Agricultural University; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, 071001, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24570454

Citation

Xie, Rong, et al. "Molecular Epidemiological Survey On Quinolone Resistance Genotype and Phenotype of Escherichia Coli in Septicemic Broilers in Hebei, China." Poultry Science, vol. 93, no. 2, 2014, pp. 335-9.
Xie R, Huo S, Li Y, et al. Molecular epidemiological survey on quinolone resistance genotype and phenotype of Escherichia coli in septicemic broilers in Hebei, China. Poult Sci. 2014;93(2):335-9.
Xie, R., Huo, S., Li, Y., Chen, L., Zhang, F., & Wu, X. (2014). Molecular epidemiological survey on quinolone resistance genotype and phenotype of Escherichia coli in septicemic broilers in Hebei, China. Poultry Science, 93(2), 335-9. https://doi.org/10.3382/ps.2013-03522
Xie R, et al. Molecular Epidemiological Survey On Quinolone Resistance Genotype and Phenotype of Escherichia Coli in Septicemic Broilers in Hebei, China. Poult Sci. 2014;93(2):335-9. PubMed PMID: 24570454.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiological survey on quinolone resistance genotype and phenotype of Escherichia coli in septicemic broilers in Hebei, China. AU - Xie,Rong, AU - Huo,Shuying, AU - Li,Yurong, AU - Chen,Ligong, AU - Zhang,Feiyan, AU - Wu,Xianjun, PY - 2014/2/27/entrez PY - 2014/2/27/pubmed PY - 2014/5/3/medline KW - Escherichia coli KW - PCR KW - broiler KW - quinolone resistance KW - resistance gene SP - 335 EP - 9 JF - Poultry science JO - Poult Sci VL - 93 IS - 2 N2 - In this study, the quinolone-resistant determining region (QRDR) of gyrA of Escherichia coli and plasmid-mediated quinolone resistance (PMQR) genes, qnr(qnrA, qnrB, and qnrS), and aac(6 ')-Ib-cr were detected, sequenced, and analyzed. In addition, antimicrobial susceptibility tests (using the Kirby-Bauer disc diffusion method) were performed for all 111 E. coli isolates from septicemic broilers in Hebei, China. The results show that the resistance rates were as follows: ofloxacin 99.10%, ciprofloxacin 93.69%, levofloxacin 91.89%, norfloxacin 90.09%, and gatifloxacin 76.58%. Of the PMQR genes examined, aac(6 ')-Ib-cr (36.04%) was the most frequently identified gene in all isolates, followed by qnrS (8.11%), qnrB (0.90%), and qnrA (0%). Of the QRDR examined in the 40 phenotypic quinolone-resistant isolates, compared with the gyrA(+) gene of E. coli K-12, 4 amino acid exchanges were found, namely Ser-83→Asp, Asp-87→Asn, Asp-87→Tyr, and Asp-87→Ala, and all 40 isolates had 1 or 2 exchanges in QRDR. It was concluded that quinolone-resistance in E. coli remains a serious problem in Hebei, China. Therefore, there is considerable local surveillance of quinolone resistance. Plasmid-mediated quinolone resistance of the qnr type remains rare in Hebei, China, and mutation in QRDR may be the main problem. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/24570454/Molecular_epidemiological_survey_on_quinolone_resistance_genotype_and_phenotype_of_Escherichia_coli_in_septicemic_broilers_in_Hebei_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)36013-4 DB - PRIME DP - Unbound Medicine ER -