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Citrus tristeza virus-based RNAi in citrus plants induces gene silencing in Diaphorina citri, a phloem-sap sucking insect vector of citrus greening disease (Huanglongbing).
J Biotechnol. 2014 Apr 20; 176:42-9.JB

Abstract

A transient expression vector based on Citrus tristeza virus (CTV) is unusually stable. Because of its stability it is being considered for use in the field to control Huanglongbing (HLB), which is caused by Candidatus Liberibacter asiaticus (CLas) and vectored by Asian citrus psyllid, Diaphorina citri. In the absence of effective control strategies for CLas, emphasis has been on control of D. citri. Coincident cohabitation in phloem tissue by CLas, D. citri and CTV was exploited to develop a novel method to mitigate HLB through RNA interference (RNAi). Since CTV has three RNA silencing suppressors, it was not known if CTV-based vector could induce RNAi in citrus. Yet, expression of sequences targeting citrus phytoene desaturase gene by CTV-RNAi resulted in photo-bleaching phenotype. CTV-RNAi vector, engineered with truncated abnormal wing disc (Awd) gene of D. citri, induced altered Awd expression when silencing triggers ingested by feeding D. citri nymphs. Decreased Awd in nymphs resulted in malformed-wing phenotype in adults and increased adult mortality. This impaired ability of D. citri to fly would potentially limit the successful vectoring of CLas bacteria between citrus trees in the grove. CTV-RNAi vector would be relevant for fast-track screening of candidate sequences for RNAi-mediated pest control.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Hajeri S
Department of Plant Pathology, University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
Killiny N
Department of Entomology and Nematology, University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
El-Mohtar C
Department of Plant Pathology, University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
Dawson WO
Department of Plant Pathology, University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
Gowda S
Department of Plant Pathology, University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA. Electronic address: gowda@ufl.edu.

MeSH

AnimalsCitrusClosterovirusGene SilencingGenes, InsectHemipteraNymphOxidoreductasesPhloemPlant DiseasesPlant ProteinsRNA InterferenceRNA, ViralRhizobiaceae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24572372

Citation

Hajeri, Subhas, et al. "Citrus Tristeza Virus-based RNAi in Citrus Plants Induces Gene Silencing in Diaphorina Citri, a Phloem-sap Sucking Insect Vector of Citrus Greening Disease (Huanglongbing)." Journal of Biotechnology, vol. 176, 2014, pp. 42-9.
Hajeri S, Killiny N, El-Mohtar C, et al. Citrus tristeza virus-based RNAi in citrus plants induces gene silencing in Diaphorina citri, a phloem-sap sucking insect vector of citrus greening disease (Huanglongbing). J Biotechnol. 2014;176:42-9.
Hajeri, S., Killiny, N., El-Mohtar, C., Dawson, W. O., & Gowda, S. (2014). Citrus tristeza virus-based RNAi in citrus plants induces gene silencing in Diaphorina citri, a phloem-sap sucking insect vector of citrus greening disease (Huanglongbing). Journal of Biotechnology, 176, 42-9. https://doi.org/10.1016/j.jbiotec.2014.02.010
Hajeri S, et al. Citrus Tristeza Virus-based RNAi in Citrus Plants Induces Gene Silencing in Diaphorina Citri, a Phloem-sap Sucking Insect Vector of Citrus Greening Disease (Huanglongbing). J Biotechnol. 2014 Apr 20;176:42-9. PubMed PMID: 24572372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Citrus tristeza virus-based RNAi in citrus plants induces gene silencing in Diaphorina citri, a phloem-sap sucking insect vector of citrus greening disease (Huanglongbing). AU - Hajeri,Subhas, AU - Killiny,Nabil, AU - El-Mohtar,Choaa, AU - Dawson,William O, AU - Gowda,Siddarame, Y1 - 2014/02/23/ PY - 2013/12/10/received PY - 2014/01/16/revised PY - 2014/02/12/accepted PY - 2014/2/28/entrez PY - 2014/2/28/pubmed PY - 2014/11/19/medline KW - Asian citrus psyllid (ACP) KW - Citrus tristeza virus KW - RNAi pest management KW - Virus-based plant mediated-RNAi KW - Virus-induced gene silencing (VIGS) SP - 42 EP - 9 JF - Journal of biotechnology JO - J Biotechnol VL - 176 N2 - A transient expression vector based on Citrus tristeza virus (CTV) is unusually stable. Because of its stability it is being considered for use in the field to control Huanglongbing (HLB), which is caused by Candidatus Liberibacter asiaticus (CLas) and vectored by Asian citrus psyllid, Diaphorina citri. In the absence of effective control strategies for CLas, emphasis has been on control of D. citri. Coincident cohabitation in phloem tissue by CLas, D. citri and CTV was exploited to develop a novel method to mitigate HLB through RNA interference (RNAi). Since CTV has three RNA silencing suppressors, it was not known if CTV-based vector could induce RNAi in citrus. Yet, expression of sequences targeting citrus phytoene desaturase gene by CTV-RNAi resulted in photo-bleaching phenotype. CTV-RNAi vector, engineered with truncated abnormal wing disc (Awd) gene of D. citri, induced altered Awd expression when silencing triggers ingested by feeding D. citri nymphs. Decreased Awd in nymphs resulted in malformed-wing phenotype in adults and increased adult mortality. This impaired ability of D. citri to fly would potentially limit the successful vectoring of CLas bacteria between citrus trees in the grove. CTV-RNAi vector would be relevant for fast-track screening of candidate sequences for RNAi-mediated pest control. SN - 1873-4863 UR - https://www.unboundmedicine.com/medline/citation/24572372/Citrus_tristeza_virus_based_RNAi_in_citrus_plants_induces_gene_silencing_in_Diaphorina_citri_a_phloem_sap_sucking_insect_vector_of_citrus_greening_disease__Huanglongbing__ DB - PRIME DP - Unbound Medicine ER -
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