Tags

Type your tag names separated by a space and hit enter

Polypyrimidine tract binding protein inhibits IgM pre-mRNA splicing by diverting U2 snRNA base-pairing away from the branch point.
RNA. 2014 Apr; 20(4):440-6.RNA

Abstract

The mouse immunoglobulin (IgM) pre-mRNA contains a splicing inhibitor that bears multiple binding sites for the splicing repressor polypyrimidine tract binding protein (PTB). Here we show that the inhibitor directs assembly of an ATP-dependent complex that contains PTB and U1 and U2 small nuclear RNAs (snRNAs). Unexpectedly, although U2 snRNA is present in the inhibitor complex, it is not base-paired to the branch point. We present evidence that inhibitor-bound PTB contacts U2 snRNA to promote base-pairing to an adjacent branch point-like sequence within the inhibitor, thereby preventing the U2 snRNA-branch point interaction and resulting in splicing repression. Our studies reveal a novel mechanism by which PTB represses splicing.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24572809

Citation

Zheng, Xuexiu, et al. "Polypyrimidine Tract Binding Protein Inhibits IgM pre-mRNA Splicing By Diverting U2 snRNA Base-pairing Away From the Branch Point." RNA (New York, N.Y.), vol. 20, no. 4, 2014, pp. 440-6.
Zheng X, Cho S, Moon H, et al. Polypyrimidine tract binding protein inhibits IgM pre-mRNA splicing by diverting U2 snRNA base-pairing away from the branch point. RNA. 2014;20(4):440-6.
Zheng, X., Cho, S., Moon, H., Loh, T. J., Oh, H. K., Green, M. R., & Shen, H. (2014). Polypyrimidine tract binding protein inhibits IgM pre-mRNA splicing by diverting U2 snRNA base-pairing away from the branch point. RNA (New York, N.Y.), 20(4), 440-6. https://doi.org/10.1261/rna.043737.113
Zheng X, et al. Polypyrimidine Tract Binding Protein Inhibits IgM pre-mRNA Splicing By Diverting U2 snRNA Base-pairing Away From the Branch Point. RNA. 2014;20(4):440-6. PubMed PMID: 24572809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polypyrimidine tract binding protein inhibits IgM pre-mRNA splicing by diverting U2 snRNA base-pairing away from the branch point. AU - Zheng,Xuexiu, AU - Cho,Sunghee, AU - Moon,Heegyum, AU - Loh,Tiing Jen, AU - Oh,Huyn Kyung, AU - Green,Michael R, AU - Shen,Haihong, Y1 - 2014/02/26/ PY - 2014/2/28/entrez PY - 2014/2/28/pubmed PY - 2014/5/16/medline KW - PTB KW - U2 snRNA KW - branch point KW - polypyrimidine tract binding protein KW - pre-mRNA splicing KW - splicing inhibitory complex SP - 440 EP - 6 JF - RNA (New York, N.Y.) JO - RNA VL - 20 IS - 4 N2 - The mouse immunoglobulin (IgM) pre-mRNA contains a splicing inhibitor that bears multiple binding sites for the splicing repressor polypyrimidine tract binding protein (PTB). Here we show that the inhibitor directs assembly of an ATP-dependent complex that contains PTB and U1 and U2 small nuclear RNAs (snRNAs). Unexpectedly, although U2 snRNA is present in the inhibitor complex, it is not base-paired to the branch point. We present evidence that inhibitor-bound PTB contacts U2 snRNA to promote base-pairing to an adjacent branch point-like sequence within the inhibitor, thereby preventing the U2 snRNA-branch point interaction and resulting in splicing repression. Our studies reveal a novel mechanism by which PTB represses splicing. SN - 1469-9001 UR - https://www.unboundmedicine.com/medline/citation/24572809/Polypyrimidine_tract_binding_protein_inhibits_IgM_pre_mRNA_splicing_by_diverting_U2_snRNA_base_pairing_away_from_the_branch_point_ L2 - http://www.rnajournal.org/cgi/pmidlookup?view=long&pmid=24572809 DB - PRIME DP - Unbound Medicine ER -