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Genome-wide serum microRNA expression profiling identifies serum biomarkers for Alzheimer's disease.
J Alzheimers Dis. 2014; 40(4):1017-27.JA

Abstract

Recent findings that human serum contains stably expressed microRNAs (miRNAs) have revealed a great potential of serum miRNA signature as disease fingerprints to diagnosis. Here we used genome-wide serum miRNA expression analysis to investigate the value of serum miRNAs as biomarkers for the diagnosis of Alzheimer's disease (AD). Illumina HiSeq 2000 sequencing followed by individual quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays was used to test the difference in levels of serum miRNAs between 50 AD patients and 50 controls in the screening stages. The detected serum miRNAs then were validated by qRT-PCR in 158 patients and 155 controls. MiR-98-5p, miR-885-5p, miR-483-3p, miR-342-3p, miR-191-5p, and miR-let-7d-5p displayed significantly different expression levels in AD patients compared with controls. Among the 6 miRNAs, miR-342-3p has the best sensitivity (81.5%) and specificity (70.1%) and was correlated to Mini-Mental State Examination score. This study identified six serum miRNAs that distinguish AD patients from healthy controls with high sensitivity and specificity. Serum miRNA panel (or miR-342-3p alone) may serve as a novel, noninvasive biomarker for AD.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Tan L
Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Shandong, China.
Yu JT
Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Shandong, China Department of Neurology, Qingdao Municipal Hospital, College of Medicine and Pharmaceutics, Ocean University of China, Shandong, China Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Jiangsu, China.
Tan MS
Department of Neurology, Qingdao Municipal Hospital, College of Medicine and Pharmaceutics, Ocean University of China, Shandong, China.
Liu QY
Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Shandong, China.
Wang HF
Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Jiangsu, China.
Zhang W
Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Shandong, China.
Jiang T
Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Jiangsu, China.
Tan L
Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Shandong, China Department of Neurology, Qingdao Municipal Hospital, College of Medicine and Pharmaceutics, Ocean University of China, Shandong, China Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Jiangsu, China.

MeSH

AgedAged, 80 and overAlzheimer DiseaseBiomarkersCohort StudiesFemaleGene Expression ProfilingGenomeHumansMaleMental Status ScheduleMicroRNAsROC CurveSensitivity and SpecificityStatistics, Nonparametric

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24577456

Clinical Trial Links

Successful Aging and Frailty

Citation

Tan, Lin, et al. "Genome-wide Serum microRNA Expression Profiling Identifies Serum Biomarkers for Alzheimer's Disease." Journal of Alzheimer's Disease : JAD, vol. 40, no. 4, 2014, pp. 1017-27.
Tan L, Yu JT, Tan MS, et al. Genome-wide serum microRNA expression profiling identifies serum biomarkers for Alzheimer's disease. J Alzheimers Dis. 2014;40(4):1017-27.
Tan, L., Yu, J. T., Tan, M. S., Liu, Q. Y., Wang, H. F., Zhang, W., Jiang, T., & Tan, L. (2014). Genome-wide serum microRNA expression profiling identifies serum biomarkers for Alzheimer's disease. Journal of Alzheimer's Disease : JAD, 40(4), 1017-27. https://doi.org/10.3233/JAD-132144
Tan L, et al. Genome-wide Serum microRNA Expression Profiling Identifies Serum Biomarkers for Alzheimer's Disease. J Alzheimers Dis. 2014;40(4):1017-27. PubMed PMID: 24577456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide serum microRNA expression profiling identifies serum biomarkers for Alzheimer's disease. AU - Tan,Lin, AU - Yu,Jin-Tai, AU - Tan,Meng-Shan, AU - Liu,Qiu-Yan, AU - Wang,Hui-Fu, AU - Zhang,Wei, AU - Jiang,Teng, AU - Tan,Lan, PY - 2014/3/1/entrez PY - 2014/3/1/pubmed PY - 2015/1/7/medline KW - Alzheimer's disease KW - biomarker KW - diagnosis KW - genome-wide sequencing KW - microRNA KW - serum SP - 1017 EP - 27 JF - Journal of Alzheimer's disease : JAD JO - J Alzheimers Dis VL - 40 IS - 4 N2 - Recent findings that human serum contains stably expressed microRNAs (miRNAs) have revealed a great potential of serum miRNA signature as disease fingerprints to diagnosis. Here we used genome-wide serum miRNA expression analysis to investigate the value of serum miRNAs as biomarkers for the diagnosis of Alzheimer's disease (AD). Illumina HiSeq 2000 sequencing followed by individual quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays was used to test the difference in levels of serum miRNAs between 50 AD patients and 50 controls in the screening stages. The detected serum miRNAs then were validated by qRT-PCR in 158 patients and 155 controls. MiR-98-5p, miR-885-5p, miR-483-3p, miR-342-3p, miR-191-5p, and miR-let-7d-5p displayed significantly different expression levels in AD patients compared with controls. Among the 6 miRNAs, miR-342-3p has the best sensitivity (81.5%) and specificity (70.1%) and was correlated to Mini-Mental State Examination score. This study identified six serum miRNAs that distinguish AD patients from healthy controls with high sensitivity and specificity. Serum miRNA panel (or miR-342-3p alone) may serve as a novel, noninvasive biomarker for AD. SN - 1875-8908 UR - https://www.unboundmedicine.com/medline/citation/24577456/Genome_wide_serum_microRNA_expression_profiling_identifies_serum_biomarkers_for_Alzheimer's_disease_ DB - PRIME DP - Unbound Medicine ER -