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microRNA156-targeted SPL/SBP box transcription factors regulate tomato ovary and fruit development.
Plant J. 2014 May; 78(4):604-18.PJ

Abstract

Fruit ripening in tomato (Solanum lycopersicum L.) is well understood at the molecular level. However, information regarding genetic pathways associated with tomato ovary and early fruit development is still lacking. Here, we investigate the possible role(s) of the microRNA156/SQUAMOSA promoter-binding protein-like (SPL or SBP box) module (miR156 node) in tomato ovary development. miR156-targeted S. lycopersicum SBP genes were dynamically expressed in developing flowers and ovaries, and miR156 was mainly expressed in meristematic tissues of the ovary, including placenta and ovules. Transgenic tomato cv. Micro-Tom plants over-expressing the AtMIR156b precursor exhibited abnormal flower and fruit morphology, with fruits characterized by growth of extra carpels and ectopic structures. Scanning electron microscopy and histological analyses showed the presence of meristem-like structures inside the ovaries, which are probably responsible for the ectopic organs. Interestingly, expression of genes associated with meristem maintenance and formation of new organs, such as LeT6/TKN2 (a KNOX-like class I gene) and GOBLET (a NAM/CUC-like gene), was induced in developing ovaries of transgenic plants as well as in the ovaries of the natural mutant Mouse ear (Me), which also displays fruits with extra carpels. Conversely, expression of the MADS box genes MACROCALYX (MC) and FUL1/TDR4, and the LEAFY ortholog FALSIFLORA, was repressed in the developing ovaries of miR156 over-expressors, suggesting similarities with Arabidopsis at this point of the miR156/SPL pathway but with distinct functional consequences in reproductive development. Altogether, these observations suggest that the miR156 node is involved in maintenance of the meristematic state of ovary tissues, thereby controlling initial steps of fleshy fruit development and determinacy.

Authors+Show Affiliations

Laboratory of Molecular Genetics of Plant Development, Department of Genetics, Instituto de Biociências, State University of Sao Paulo, Botucatu, Sao Paulo, 18618-970, Brazil; Centro de Biotecnologia Agricola, Escola Superior de Agricultura 'Luiz de Queiroz', University of Sao Paulo, 13418-900, Piracicaba, Sao Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24580734

Citation

Ferreira e Silva, Geraldo Felipe, et al. "MicroRNA156-targeted SPL/SBP Box Transcription Factors Regulate Tomato Ovary and Fruit Development." The Plant Journal : for Cell and Molecular Biology, vol. 78, no. 4, 2014, pp. 604-18.
Ferreira e Silva GF, Silva EM, Azevedo Mda S, et al. MicroRNA156-targeted SPL/SBP box transcription factors regulate tomato ovary and fruit development. Plant J. 2014;78(4):604-18.
Ferreira e Silva, G. F., Silva, E. M., Azevedo, M. d. a. . S., Guivin, M. A., Ramiro, D. A., Figueiredo, C. R., Carrer, H., Peres, L. E., & Nogueira, F. T. (2014). MicroRNA156-targeted SPL/SBP box transcription factors regulate tomato ovary and fruit development. The Plant Journal : for Cell and Molecular Biology, 78(4), 604-18. https://doi.org/10.1111/tpj.12493
Ferreira e Silva GF, et al. MicroRNA156-targeted SPL/SBP Box Transcription Factors Regulate Tomato Ovary and Fruit Development. Plant J. 2014;78(4):604-18. PubMed PMID: 24580734.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - microRNA156-targeted SPL/SBP box transcription factors regulate tomato ovary and fruit development. AU - Ferreira e Silva,Geraldo Felipe, AU - Silva,Eder Marques, AU - Azevedo,Mariana da Silva, AU - Guivin,Mike Anderson Corazon, AU - Ramiro,Daniel Alves, AU - Figueiredo,Cassia Regina, AU - Carrer,Helaine, AU - Peres,Lázaro Eustáquio Pereira, AU - Nogueira,Fabio Tebaldi Silveira, Y1 - 2014/04/07/ PY - 2013/07/29/received PY - 2014/01/18/revised PY - 2014/02/19/accepted PY - 2014/3/4/entrez PY - 2014/3/4/pubmed PY - 2015/2/20/medline KW - SPL/SBPs KW - Solanum lycopersicum KW - fruit patterning KW - gynoecium KW - microRNA156 KW - ovary SP - 604 EP - 18 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 78 IS - 4 N2 - Fruit ripening in tomato (Solanum lycopersicum L.) is well understood at the molecular level. However, information regarding genetic pathways associated with tomato ovary and early fruit development is still lacking. Here, we investigate the possible role(s) of the microRNA156/SQUAMOSA promoter-binding protein-like (SPL or SBP box) module (miR156 node) in tomato ovary development. miR156-targeted S. lycopersicum SBP genes were dynamically expressed in developing flowers and ovaries, and miR156 was mainly expressed in meristematic tissues of the ovary, including placenta and ovules. Transgenic tomato cv. Micro-Tom plants over-expressing the AtMIR156b precursor exhibited abnormal flower and fruit morphology, with fruits characterized by growth of extra carpels and ectopic structures. Scanning electron microscopy and histological analyses showed the presence of meristem-like structures inside the ovaries, which are probably responsible for the ectopic organs. Interestingly, expression of genes associated with meristem maintenance and formation of new organs, such as LeT6/TKN2 (a KNOX-like class I gene) and GOBLET (a NAM/CUC-like gene), was induced in developing ovaries of transgenic plants as well as in the ovaries of the natural mutant Mouse ear (Me), which also displays fruits with extra carpels. Conversely, expression of the MADS box genes MACROCALYX (MC) and FUL1/TDR4, and the LEAFY ortholog FALSIFLORA, was repressed in the developing ovaries of miR156 over-expressors, suggesting similarities with Arabidopsis at this point of the miR156/SPL pathway but with distinct functional consequences in reproductive development. Altogether, these observations suggest that the miR156 node is involved in maintenance of the meristematic state of ovary tissues, thereby controlling initial steps of fleshy fruit development and determinacy. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/24580734/microRNA156_targeted_SPL/SBP_box_transcription_factors_regulate_tomato_ovary_and_fruit_development_ L2 - https://doi.org/10.1111/tpj.12493 DB - PRIME DP - Unbound Medicine ER -