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Anti-diabetic effects of the acetone fraction of Senna singueana stem bark in a type 2 diabetes rat model.
J Ethnopharmacol. 2014 Apr 28; 153(2):392-9.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Senna singueana is currently used in the traditional treatment of diabetes mellitus in Nigeria. The present study examined the anti-diabetic activity of the Senna singueana acetone fraction (SSAF) of stem bark in a type 2 diabetes (T2D) rat model.

MATERIALS AND METHODS

Crude ethyl acetate extract of the Senna singueana stem bark was fractionated with various solvents and the acetone fraction was selected for in vivo studies based on the high α-glucosidase and α-amylase inhibitory activities. In the in vivo study, male Sprague-Dawley rats were induced with T2D and treated with the SSAF at 150 and 300 mg/kg body weight. Several T2D-related parameters were measured in the study.

RESULTS

After 4 weeks of intervention, non-fasting blood glucose concentrations were significantly decreased and the glucose tolerance ability was significantly improved in the SSAF treated groups compared to the diabetic control group. Serum insulin concentrations, pancreatic β-cell function (HOMA-β) and liver glycogen were significantly (P<0.05) increased while serum alanine transaminase, alkaline phosphatase and urea were significantly decreased in the SSAF treated diabetic rats compared to the diabetic control group. Though insignificantly (P>0.05), other T2D-induced abnormalities such as food and fluid intake, body weight, serum lipids, serum fructosamine level and peripheral insulin resistance (HOMA-IR) were also partially ameliorated by the SSAF treatment.

CONCLUSION

Data of this study suggest that orally administered SSAF could ameliorate most of the T2D-induced abnormalities in a T2D model of rats.

Authors+Show Affiliations

Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban 4000, South Africa; Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban 4000, South Africa. Electronic address: islamd@ukzn.ac.za.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24583108

Citation

Ibrahim, Mohammed Auwal, and M Shahidul Islam. "Anti-diabetic Effects of the Acetone Fraction of Senna Singueana Stem Bark in a Type 2 Diabetes Rat Model." Journal of Ethnopharmacology, vol. 153, no. 2, 2014, pp. 392-9.
Ibrahim MA, Islam MS. Anti-diabetic effects of the acetone fraction of Senna singueana stem bark in a type 2 diabetes rat model. J Ethnopharmacol. 2014;153(2):392-9.
Ibrahim, M. A., & Islam, M. S. (2014). Anti-diabetic effects of the acetone fraction of Senna singueana stem bark in a type 2 diabetes rat model. Journal of Ethnopharmacology, 153(2), 392-9. https://doi.org/10.1016/j.jep.2014.02.042
Ibrahim MA, Islam MS. Anti-diabetic Effects of the Acetone Fraction of Senna Singueana Stem Bark in a Type 2 Diabetes Rat Model. J Ethnopharmacol. 2014 Apr 28;153(2):392-9. PubMed PMID: 24583108.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-diabetic effects of the acetone fraction of Senna singueana stem bark in a type 2 diabetes rat model. AU - Ibrahim,Mohammed Auwal, AU - Islam,M Shahidul, Y1 - 2014/02/26/ PY - 2013/09/03/received PY - 2014/01/28/revised PY - 2014/02/18/accepted PY - 2014/3/4/entrez PY - 2014/3/4/pubmed PY - 2014/12/17/medline KW - Absolute ethanol (CID 702) KW - Dinitrosalicylic acid (DNS) (CID 11873) KW - Ethyl acetate (CID 8857) KW - Hydrogen peroxide (CID 784) KW - Maltose (CID 6255) KW - Rats KW - Senna singueana KW - Starch (CID 439341) KW - Streptozotocin (CID 29327) KW - Type 2 diabetes KW - p-Nitrophenol (CID 980) KW - p-Nitrophenyl-α-d-glucopyranoside (pNPG) (CID 11197369) KW - α-Amylase KW - α-Amylase (E.C. 3.2.1.1) KW - α-Glucosidase KW - α-Glucosidase (E.C. 3.2.1.20) SP - 392 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 153 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Senna singueana is currently used in the traditional treatment of diabetes mellitus in Nigeria. The present study examined the anti-diabetic activity of the Senna singueana acetone fraction (SSAF) of stem bark in a type 2 diabetes (T2D) rat model. MATERIALS AND METHODS: Crude ethyl acetate extract of the Senna singueana stem bark was fractionated with various solvents and the acetone fraction was selected for in vivo studies based on the high α-glucosidase and α-amylase inhibitory activities. In the in vivo study, male Sprague-Dawley rats were induced with T2D and treated with the SSAF at 150 and 300 mg/kg body weight. Several T2D-related parameters were measured in the study. RESULTS: After 4 weeks of intervention, non-fasting blood glucose concentrations were significantly decreased and the glucose tolerance ability was significantly improved in the SSAF treated groups compared to the diabetic control group. Serum insulin concentrations, pancreatic β-cell function (HOMA-β) and liver glycogen were significantly (P<0.05) increased while serum alanine transaminase, alkaline phosphatase and urea were significantly decreased in the SSAF treated diabetic rats compared to the diabetic control group. Though insignificantly (P>0.05), other T2D-induced abnormalities such as food and fluid intake, body weight, serum lipids, serum fructosamine level and peripheral insulin resistance (HOMA-IR) were also partially ameliorated by the SSAF treatment. CONCLUSION: Data of this study suggest that orally administered SSAF could ameliorate most of the T2D-induced abnormalities in a T2D model of rats. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24583108/Anti_diabetic_effects_of_the_acetone_fraction_of_Senna_singueana_stem_bark_in_a_type_2_diabetes_rat_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(14)00155-X DB - PRIME DP - Unbound Medicine ER -