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Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells.
Virus Res. 2014 May 12; 184:39-43.VR

Abstract

The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV-2 were performed in mosquito C6/36 cells or monkey Vero cells and the antiviral susceptibility of each virus passage to sulfated polysaccharides (SPs), like heparin and carrageenans, was evaluated by a virus plaque reduction assay. After serial passaging in Vero cells, DENV-2 became increasingly resistant to SP inhibition whereas the antiviral susceptibility was not altered in virus propagated in C6/36 cells. The change in antiviral susceptibility was associated to a differential mode of entry into the host cell. The route of endocytic entry for productive Vero cell infection was altered from a non-classical clathrin independent pathway for C6/36-grown virus to a clathrin-mediated endocytosis when the virus was serially propagated in Vero cells. Our results show the impact of the cellular system used for successive propagation of DENV on the initial interaction between the host cell and the virion in the next round of infection and the relevant consequences it might have during the in vitro evaluation of entry inhibitors.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Acosta EG
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Piccini LE
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina; IQUIBICEN-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Universitaria, Pabellón 2, Piso 4, 1428 Buenos Aires, Argentina.
Talarico LB
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Castilla V
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Damonte EB
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina; IQUIBICEN-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Universitaria, Pabellón 2, Piso 4, 1428 Buenos Aires, Argentina. Electronic address: edamonte@qb.fcen.uba.ar.

MeSH

Adaptation, BiologicalAnimalsAntiviral AgentsCarrageenanCell LineChlorocebus aethiopsCulicidaeDNA Mutational AnalysisDengue VirusDrug Resistance, ViralEndocytosisHeparinMolecular Sequence DataRNA, ViralSequence Analysis, DNASerial PassageViral Plaque AssayVirus Internalization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24583230

Citation

Acosta, Eliana G., et al. "Changes in Antiviral Susceptibility to Entry Inhibitors and Endocytic Uptake of Dengue-2 Virus Serially Passaged in Vero or C6/36 Cells." Virus Research, vol. 184, 2014, pp. 39-43.
Acosta EG, Piccini LE, Talarico LB, et al. Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells. Virus Res. 2014;184:39-43.
Acosta, E. G., Piccini, L. E., Talarico, L. B., Castilla, V., & Damonte, E. B. (2014). Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells. Virus Research, 184, 39-43. https://doi.org/10.1016/j.virusres.2014.02.011
Acosta EG, et al. Changes in Antiviral Susceptibility to Entry Inhibitors and Endocytic Uptake of Dengue-2 Virus Serially Passaged in Vero or C6/36 Cells. Virus Res. 2014 May 12;184:39-43. PubMed PMID: 24583230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells. AU - Acosta,Eliana G, AU - Piccini,Luana E, AU - Talarico,Laura B, AU - Castilla,Viviana, AU - Damonte,Elsa B, Y1 - 2014/02/25/ PY - 2013/12/05/received PY - 2014/01/24/revised PY - 2014/02/17/accepted PY - 2014/3/4/entrez PY - 2014/3/4/pubmed PY - 2014/12/15/medline KW - Antiviral activity KW - Carrageenan KW - Clathrin-mediated endocytosis KW - Dengue virus KW - Entry inhibitor KW - Sulfated polysaccharide SP - 39 EP - 43 JF - Virus research JO - Virus Res VL - 184 N2 - The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV-2 were performed in mosquito C6/36 cells or monkey Vero cells and the antiviral susceptibility of each virus passage to sulfated polysaccharides (SPs), like heparin and carrageenans, was evaluated by a virus plaque reduction assay. After serial passaging in Vero cells, DENV-2 became increasingly resistant to SP inhibition whereas the antiviral susceptibility was not altered in virus propagated in C6/36 cells. The change in antiviral susceptibility was associated to a differential mode of entry into the host cell. The route of endocytic entry for productive Vero cell infection was altered from a non-classical clathrin independent pathway for C6/36-grown virus to a clathrin-mediated endocytosis when the virus was serially propagated in Vero cells. Our results show the impact of the cellular system used for successive propagation of DENV on the initial interaction between the host cell and the virion in the next round of infection and the relevant consequences it might have during the in vitro evaluation of entry inhibitors. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/24583230/Changes_in_antiviral_susceptibility_to_entry_inhibitors_and_endocytic_uptake_of_dengue_2_virus_serially_passaged_in_Vero_or_C6/36_cells_ DB - PRIME DP - Unbound Medicine ER -