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Rapid extraction and determination of amphetamines in human urine samples using dispersive liquid-liquid microextraction and solidification of floating organic drop followed by high performance liquid chromatography.
J Pharm Biomed Anal. 2014 Jun; 94:145-51.JP

Abstract

A novel, rapid, simple and sensitive dispersive liquid-liquid microextraction method based on the solidification of floating organic drop (DLLME-SFO) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine amphetamine and methamphetamine in urine samples. The factors affecting the extraction efficiency of DLLME-SFO such as the kind and volume of the extraction and the disperser solvents, effect of concentration of K2CO3 and extraction time were investigated and the optimal extraction conditions were established. Under the optimum conditions (extraction solvent: 30.0μl 1-undecanol; disperser solvent: 300μl acetonitrile; buffer concentration: 2% (w/v) K2CO3 and extraction time: 1min), calibration curves are linear in the range of 10-3000μgl(-1) and limit of detections (LODs) are in the range of 2-8μgl(-1). The relative standard deviations (RSDs) for 100μgl(-1) of amphetamine and methamphetamine in diluted urine are in the range of 6.2-7.8% (n=7). The method was successfully applied for the determination of amphetamine and methamphetamine in the actual urine samples. The relative recoveries of urine samples spiked with amphetamine and methamphetamine are 87.8-113.2%. The obtained results show that DLLME-SFO combined with HPLC-UV is a fast and simple method for the determination of amphetamine and methamphetamine in urine.

Authors+Show Affiliations

Iran West Center for Drug Abuse Studies, Kermanshah University of Medical Sciences, Kermanshah, Iran.Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of Medical Sciences, Kermanshah, Iran. Electronic address: nazirfatahi@yahoo.com.Department Chemistry, Razi University, Kermanshah, Iran.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24583909

Citation

Ahmadi-Jouibari, Toraj, et al. "Rapid Extraction and Determination of Amphetamines in Human Urine Samples Using Dispersive Liquid-liquid Microextraction and Solidification of Floating Organic Drop Followed By High Performance Liquid Chromatography." Journal of Pharmaceutical and Biomedical Analysis, vol. 94, 2014, pp. 145-51.
Ahmadi-Jouibari T, Fattahi N, Shamsipur M. Rapid extraction and determination of amphetamines in human urine samples using dispersive liquid-liquid microextraction and solidification of floating organic drop followed by high performance liquid chromatography. J Pharm Biomed Anal. 2014;94:145-51.
Ahmadi-Jouibari, T., Fattahi, N., & Shamsipur, M. (2014). Rapid extraction and determination of amphetamines in human urine samples using dispersive liquid-liquid microextraction and solidification of floating organic drop followed by high performance liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis, 94, 145-51. https://doi.org/10.1016/j.jpba.2014.01.044
Ahmadi-Jouibari T, Fattahi N, Shamsipur M. Rapid Extraction and Determination of Amphetamines in Human Urine Samples Using Dispersive Liquid-liquid Microextraction and Solidification of Floating Organic Drop Followed By High Performance Liquid Chromatography. J Pharm Biomed Anal. 2014;94:145-51. PubMed PMID: 24583909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid extraction and determination of amphetamines in human urine samples using dispersive liquid-liquid microextraction and solidification of floating organic drop followed by high performance liquid chromatography. AU - Ahmadi-Jouibari,Toraj, AU - Fattahi,Nazir, AU - Shamsipur,Mojtaba, Y1 - 2014/02/05/ PY - 2014/01/11/received PY - 2014/01/26/revised PY - 2014/01/29/accepted PY - 2014/3/4/entrez PY - 2014/3/4/pubmed PY - 2014/11/18/medline KW - Amphetamines KW - Dispersive liquid–liquid microextraction KW - Solidification of floating organic drop KW - Urine analysis SP - 145 EP - 51 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 94 N2 - A novel, rapid, simple and sensitive dispersive liquid-liquid microextraction method based on the solidification of floating organic drop (DLLME-SFO) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine amphetamine and methamphetamine in urine samples. The factors affecting the extraction efficiency of DLLME-SFO such as the kind and volume of the extraction and the disperser solvents, effect of concentration of K2CO3 and extraction time were investigated and the optimal extraction conditions were established. Under the optimum conditions (extraction solvent: 30.0μl 1-undecanol; disperser solvent: 300μl acetonitrile; buffer concentration: 2% (w/v) K2CO3 and extraction time: 1min), calibration curves are linear in the range of 10-3000μgl(-1) and limit of detections (LODs) are in the range of 2-8μgl(-1). The relative standard deviations (RSDs) for 100μgl(-1) of amphetamine and methamphetamine in diluted urine are in the range of 6.2-7.8% (n=7). The method was successfully applied for the determination of amphetamine and methamphetamine in the actual urine samples. The relative recoveries of urine samples spiked with amphetamine and methamphetamine are 87.8-113.2%. The obtained results show that DLLME-SFO combined with HPLC-UV is a fast and simple method for the determination of amphetamine and methamphetamine in urine. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/24583909/Rapid_extraction_and_determination_of_amphetamines_in_human_urine_samples_using_dispersive_liquid_liquid_microextraction_and_solidification_of_floating_organic_drop_followed_by_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(14)00081-8 DB - PRIME DP - Unbound Medicine ER -