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Quantitative label-free phosphoproteomics of six different life stages of the late blight pathogen Phytophthora infestans reveals abundant phosphorylation of members of the CRN effector family.
J Proteome Res. 2014 Apr 04; 13(4):1848-59.JP

Abstract

The oomycete Phytophthora infestans is the causal agent of late blight in potato and tomato. Since the underlying processes that govern pathogenicity and development in P. infestans are largely unknown, we have performed a large-scale phosphoproteomics study of six different P. infestans life stages. We have obtained quantitative data for 2922 phosphopeptides and compared their abundance. Life-stage-specific phosphopeptides include ATP-binding cassette transporters and a kinase that only occurs in appressoria. In an extended data set, we identified 2179 phosphorylation sites and deduced 22 phosphomotifs. Several of the phosphomotifs matched consensus sequences of kinases that occur in P. infestans but not Arabidopsis. In addition, we detected tyrosine phosphopeptides that are potential targets of kinases resembling mammalian tyrosine kinases. Among the phosphorylated proteins are members of the RXLR and Crinkler effector families. The latter are phosphorylated in several life stages and at multiple positions, in sites that are conserved between different members of the Crinkler family. This indicates that proteins in the Crinkler family have functions beyond their putative role as (necrosis-inducing) effectors. This phosphoproteomics data will be instrumental for studies on oomycetes and host-oomycete interactions. The data sets have been deposited to ProteomeXchange (identifier PXD000433).

Authors+Show Affiliations

Department of Plant Protection Biology, Swedish University of Agricultural Sciences , SE-230 53 Alnarp, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24588563

Citation

Resjö, Svante, et al. "Quantitative Label-free Phosphoproteomics of Six Different Life Stages of the Late Blight Pathogen Phytophthora Infestans Reveals Abundant Phosphorylation of Members of the CRN Effector Family." Journal of Proteome Research, vol. 13, no. 4, 2014, pp. 1848-59.
Resjö S, Ali A, Meijer HJ, et al. Quantitative label-free phosphoproteomics of six different life stages of the late blight pathogen Phytophthora infestans reveals abundant phosphorylation of members of the CRN effector family. J Proteome Res. 2014;13(4):1848-59.
Resjö, S., Ali, A., Meijer, H. J., Seidl, M. F., Snel, B., Sandin, M., Levander, F., Govers, F., & Andreasson, E. (2014). Quantitative label-free phosphoproteomics of six different life stages of the late blight pathogen Phytophthora infestans reveals abundant phosphorylation of members of the CRN effector family. Journal of Proteome Research, 13(4), 1848-59. https://doi.org/10.1021/pr4009095
Resjö S, et al. Quantitative Label-free Phosphoproteomics of Six Different Life Stages of the Late Blight Pathogen Phytophthora Infestans Reveals Abundant Phosphorylation of Members of the CRN Effector Family. J Proteome Res. 2014 Apr 4;13(4):1848-59. PubMed PMID: 24588563.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative label-free phosphoproteomics of six different life stages of the late blight pathogen Phytophthora infestans reveals abundant phosphorylation of members of the CRN effector family. AU - Resjö,Svante, AU - Ali,Ashfaq, AU - Meijer,Harold J G, AU - Seidl,Michael F, AU - Snel,Berend, AU - Sandin,Marianne, AU - Levander,Fredrik, AU - Govers,Francine, AU - Andreasson,Erik, Y1 - 2014/03/17/ PY - 2014/3/5/entrez PY - 2014/3/5/pubmed PY - 2014/12/15/medline SP - 1848 EP - 59 JF - Journal of proteome research JO - J. Proteome Res. VL - 13 IS - 4 N2 - The oomycete Phytophthora infestans is the causal agent of late blight in potato and tomato. Since the underlying processes that govern pathogenicity and development in P. infestans are largely unknown, we have performed a large-scale phosphoproteomics study of six different P. infestans life stages. We have obtained quantitative data for 2922 phosphopeptides and compared their abundance. Life-stage-specific phosphopeptides include ATP-binding cassette transporters and a kinase that only occurs in appressoria. In an extended data set, we identified 2179 phosphorylation sites and deduced 22 phosphomotifs. Several of the phosphomotifs matched consensus sequences of kinases that occur in P. infestans but not Arabidopsis. In addition, we detected tyrosine phosphopeptides that are potential targets of kinases resembling mammalian tyrosine kinases. Among the phosphorylated proteins are members of the RXLR and Crinkler effector families. The latter are phosphorylated in several life stages and at multiple positions, in sites that are conserved between different members of the Crinkler family. This indicates that proteins in the Crinkler family have functions beyond their putative role as (necrosis-inducing) effectors. This phosphoproteomics data will be instrumental for studies on oomycetes and host-oomycete interactions. The data sets have been deposited to ProteomeXchange (identifier PXD000433). SN - 1535-3907 UR - https://www.unboundmedicine.com/medline/citation/24588563/Quantitative_label_free_phosphoproteomics_of_six_different_life_stages_of_the_late_blight_pathogen_Phytophthora_infestans_reveals_abundant_phosphorylation_of_members_of_the_CRN_effector_family_ L2 - https://dx.doi.org/10.1021/pr4009095 DB - PRIME DP - Unbound Medicine ER -