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Evaluation of the impact of tromethamine edetate disodium dihydrate on antimicrobial susceptibility of Pseudomonas aeruginosa in biofilm in vitro.
Vet Dermatol. 2014 Apr; 25(2):120-3, e33-4.VD

Abstract

BACKGROUND

Biofilm formation by Pseudomonas aeruginosa has been documented in canine otic isolates. An increase in minimal inhibitory concentration (MIC) for specific antibiotics has been noted for biofilm-embedded bacteria. Tromethamine edetate disodium dihydrate buffered to pH 8 with tromethamine hydrochloride and deionized water (Triz-EDTA(®)) has been documented to potentiate bactericidal activity when used in combination with topical antibiotics, but the impact on biofilm-embedded bacteria is unknown.

HYPOTHESIS/OBJECTIVES

The objective of this study was to evaluate the impact of Triz-EDTA(®) use on in vitro antimicrobial susceptibility of biofilm-embedded P. aeruginosa.

METHODS

Biofilm formation was documented using a microtitre plate assay. Broth microdilution was used to assess the MIC of neomycin, polymyxin B, enrofloxacin and gentamicin for the biofilm-embedded bacteria. The microtitre plate assay was again used to assess the MIC of neomycin, polymyxin B, enrofloxacin and gentamicin for biofilm-embedded bacteria with added Triz-EDTA(®).

RESULTS

Thirty-one isolates from dogs with otitis were tested. Addition of Triz-EDTA(®) significantly reduced MICs for neomycin (P < 0.003) and gentamicin (P < 0.02) but not for polymyxin B (P = 0.3). Enrofloxacin MICs increased in the presence of Triz-EDTA (P < 0.036).

CONCLUSIONS AND CLINICAL IMPORTANCE

Triz-EDTA(®) may be a useful adjunctive treatment for chronic cases of Pseudomonas otitis where biofilms may have developed, if gentamicin or neomycin is to be used as a topical treatment. In vivo study is required to confirm this effect.

Authors+Show Affiliations

Yu of Guelph Veterinary Dermatology, Guelph Veterinary Specialty Hospital, 1460 Gordon Street South, Guelph, Ontario, N1L 1C8.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24589016

Citation

Pye, Charlotte C., et al. "Evaluation of the Impact of Tromethamine Edetate Disodium Dihydrate On Antimicrobial Susceptibility of Pseudomonas Aeruginosa in Biofilm in Vitro." Veterinary Dermatology, vol. 25, no. 2, 2014, pp. 120-3, e33-4.
Pye CC, Singh A, Weese JS. Evaluation of the impact of tromethamine edetate disodium dihydrate on antimicrobial susceptibility of Pseudomonas aeruginosa in biofilm in vitro. Vet Dermatol. 2014;25(2):120-3, e33-4.
Pye, C. C., Singh, A., & Weese, J. S. (2014). Evaluation of the impact of tromethamine edetate disodium dihydrate on antimicrobial susceptibility of Pseudomonas aeruginosa in biofilm in vitro. Veterinary Dermatology, 25(2), 120-3, e33-4. https://doi.org/10.1111/vde.12115
Pye CC, Singh A, Weese JS. Evaluation of the Impact of Tromethamine Edetate Disodium Dihydrate On Antimicrobial Susceptibility of Pseudomonas Aeruginosa in Biofilm in Vitro. Vet Dermatol. 2014;25(2):120-3, e33-4. PubMed PMID: 24589016.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the impact of tromethamine edetate disodium dihydrate on antimicrobial susceptibility of Pseudomonas aeruginosa in biofilm in vitro. AU - Pye,Charlotte C, AU - Singh,Ameet, AU - Weese,J Scott, Y1 - 2014/03/03/ PY - 2013/12/23/accepted PY - 2014/3/5/entrez PY - 2014/3/5/pubmed PY - 2014/12/15/medline SP - 120-3, e33-4 JF - Veterinary dermatology JO - Vet. Dermatol. VL - 25 IS - 2 N2 - BACKGROUND: Biofilm formation by Pseudomonas aeruginosa has been documented in canine otic isolates. An increase in minimal inhibitory concentration (MIC) for specific antibiotics has been noted for biofilm-embedded bacteria. Tromethamine edetate disodium dihydrate buffered to pH 8 with tromethamine hydrochloride and deionized water (Triz-EDTA(®)) has been documented to potentiate bactericidal activity when used in combination with topical antibiotics, but the impact on biofilm-embedded bacteria is unknown. HYPOTHESIS/OBJECTIVES: The objective of this study was to evaluate the impact of Triz-EDTA(®) use on in vitro antimicrobial susceptibility of biofilm-embedded P. aeruginosa. METHODS: Biofilm formation was documented using a microtitre plate assay. Broth microdilution was used to assess the MIC of neomycin, polymyxin B, enrofloxacin and gentamicin for the biofilm-embedded bacteria. The microtitre plate assay was again used to assess the MIC of neomycin, polymyxin B, enrofloxacin and gentamicin for biofilm-embedded bacteria with added Triz-EDTA(®). RESULTS: Thirty-one isolates from dogs with otitis were tested. Addition of Triz-EDTA(®) significantly reduced MICs for neomycin (P < 0.003) and gentamicin (P < 0.02) but not for polymyxin B (P = 0.3). Enrofloxacin MICs increased in the presence of Triz-EDTA (P < 0.036). CONCLUSIONS AND CLINICAL IMPORTANCE: Triz-EDTA(®) may be a useful adjunctive treatment for chronic cases of Pseudomonas otitis where biofilms may have developed, if gentamicin or neomycin is to be used as a topical treatment. In vivo study is required to confirm this effect. SN - 1365-3164 UR - https://www.unboundmedicine.com/medline/citation/24589016/Evaluation_of_the_impact_of_tromethamine_edetate_disodium_dihydrate_on_antimicrobial_susceptibility_of_Pseudomonas_aeruginosa_in_biofilm_in_vitro_ L2 - https://doi.org/10.1111/vde.12115 DB - PRIME DP - Unbound Medicine ER -