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Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a pandoravirus morphology.
Proc Natl Acad Sci U S A. 2014 Mar 18; 111(11):4274-9.PN

Abstract

The largest known DNA viruses infect Acanthamoeba and belong to two markedly different families. The Megaviridae exhibit pseudo-icosahedral virions up to 0.7 μm in diameter and adenine-thymine (AT)-rich genomes of up to 1.25 Mb encoding a thousand proteins. Like their Mimivirus prototype discovered 10 y ago, they entirely replicate within cytoplasmic virion factories. In contrast, the recently discovered Pandoraviruses exhibit larger amphora-shaped virions 1 μm in length and guanine-cytosine-rich genomes up to 2.8 Mb long encoding up to 2,500 proteins. Their replication involves the host nucleus. Whereas the Megaviridae share some general features with the previously described icosahedral large DNA viruses, the Pandoraviruses appear unrelated to them. Here we report the discovery of a third type of giant virus combining an even larger pandoravirus-like particle 1.5 μm in length with a surprisingly smaller 600 kb AT-rich genome, a gene content more similar to Iridoviruses and Marseillevirus, and a fully cytoplasmic replication reminiscent of the Megaviridae. This suggests that pandoravirus-like particles may be associated with a variety of virus families more diverse than previously envisioned. This giant virus, named Pithovirus sibericum, was isolated from a >30,000-y-old radiocarbon-dated sample when we initiated a survey of the virome of Siberian permafrost. The revival of such an ancestral amoeba-infecting virus used as a safe indicator of the possible presence of pathogenic DNA viruses, suggests that the thawing of permafrost either from global warming or industrial exploitation of circumpolar regions might not be exempt from future threats to human or animal health.

Authors+Show Affiliations

Structural and Genomic Information Laboratory, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée) Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille Cedex 9, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24591590

Citation

Legendre, Matthieu, et al. "Thirty-thousand-year-old Distant Relative of Giant Icosahedral DNA Viruses With a Pandoravirus Morphology." Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 11, 2014, pp. 4274-9.
Legendre M, Bartoli J, Shmakova L, et al. Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a pandoravirus morphology. Proc Natl Acad Sci U S A. 2014;111(11):4274-9.
Legendre, M., Bartoli, J., Shmakova, L., Jeudy, S., Labadie, K., Adrait, A., Lescot, M., Poirot, O., Bertaux, L., Bruley, C., Couté, Y., Rivkina, E., Abergel, C., & Claverie, J. M. (2014). Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a pandoravirus morphology. Proceedings of the National Academy of Sciences of the United States of America, 111(11), 4274-9. https://doi.org/10.1073/pnas.1320670111
Legendre M, et al. Thirty-thousand-year-old Distant Relative of Giant Icosahedral DNA Viruses With a Pandoravirus Morphology. Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4274-9. PubMed PMID: 24591590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a pandoravirus morphology. AU - Legendre,Matthieu, AU - Bartoli,Julia, AU - Shmakova,Lyubov, AU - Jeudy,Sandra, AU - Labadie,Karine, AU - Adrait,Annie, AU - Lescot,Magali, AU - Poirot,Olivier, AU - Bertaux,Lionel, AU - Bruley,Christophe, AU - Couté,Yohann, AU - Rivkina,Elizaveta, AU - Abergel,Chantal, AU - Claverie,Jean-Michel, Y1 - 2014/03/03/ PY - 2014/3/5/entrez PY - 2014/3/5/pubmed PY - 2014/5/30/medline KW - giant DNA virus KW - icosahedral capsid KW - late Pleistocene SP - 4274 EP - 9 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 111 IS - 11 N2 - The largest known DNA viruses infect Acanthamoeba and belong to two markedly different families. The Megaviridae exhibit pseudo-icosahedral virions up to 0.7 μm in diameter and adenine-thymine (AT)-rich genomes of up to 1.25 Mb encoding a thousand proteins. Like their Mimivirus prototype discovered 10 y ago, they entirely replicate within cytoplasmic virion factories. In contrast, the recently discovered Pandoraviruses exhibit larger amphora-shaped virions 1 μm in length and guanine-cytosine-rich genomes up to 2.8 Mb long encoding up to 2,500 proteins. Their replication involves the host nucleus. Whereas the Megaviridae share some general features with the previously described icosahedral large DNA viruses, the Pandoraviruses appear unrelated to them. Here we report the discovery of a third type of giant virus combining an even larger pandoravirus-like particle 1.5 μm in length with a surprisingly smaller 600 kb AT-rich genome, a gene content more similar to Iridoviruses and Marseillevirus, and a fully cytoplasmic replication reminiscent of the Megaviridae. This suggests that pandoravirus-like particles may be associated with a variety of virus families more diverse than previously envisioned. This giant virus, named Pithovirus sibericum, was isolated from a >30,000-y-old radiocarbon-dated sample when we initiated a survey of the virome of Siberian permafrost. The revival of such an ancestral amoeba-infecting virus used as a safe indicator of the possible presence of pathogenic DNA viruses, suggests that the thawing of permafrost either from global warming or industrial exploitation of circumpolar regions might not be exempt from future threats to human or animal health. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/24591590/Thirty_thousand_year_old_distant_relative_of_giant_icosahedral_DNA_viruses_with_a_pandoravirus_morphology_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=24591590 DB - PRIME DP - Unbound Medicine ER -