Tags

Type your tag names separated by a space and hit enter

Analysis of the molecular specificities of anti-class II monoclonal antibodies by using L cell transfectants expressing HLA class II molecules.
J Immunol. 1988 Sep 15; 141(6):2158-64.JI

Abstract

Expressible HLA class II alpha- and beta-chain cDNA were used for DNA-mediated gene transfer to produce L cell transfectants expressing single types of human class II molecules. Cloned transfectants expressing nine different class II molecules were isolated: DR alpha: DR1 beta I, DR alpha: DR4 beta I, DR alpha: DR5 beta I, DR alpha: DR5 beta III (DRw52), DR alpha: DR7 beta I, DR alpha: DR4/7 beta IV (DRw53), DQ7 alpha: DQw2 beta, DQ7 alpha: DQw3 beta, and DPw4 alpha: DPw4 beta. These class II-expressing transfectants were used to analyze by flow cytometry the molecular specificities of 20 anti-class II mAb. These analyes indicate that some mAb are more broadly reactive than was previously thought based on immunochemical studies. In contrast, the narrow molecular specificities of other anti-class II mAb were confirmed by this approach. Transfectants expressing human class II molecules should be valuable reagents for studies of B cell and T cell defined epitopes on these molecules.

Authors+Show Affiliations

Veterans Administration Medical Center, Iowa City 52240.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2459201

Citation

Klohe, E P., et al. "Analysis of the Molecular Specificities of Anti-class II Monoclonal Antibodies By Using L Cell Transfectants Expressing HLA Class II Molecules." Journal of Immunology (Baltimore, Md. : 1950), vol. 141, no. 6, 1988, pp. 2158-64.
Klohe EP, Watts R, Bahl M, et al. Analysis of the molecular specificities of anti-class II monoclonal antibodies by using L cell transfectants expressing HLA class II molecules. J Immunol. 1988;141(6):2158-64.
Klohe, E. P., Watts, R., Bahl, M., Alber, C., Yu, W. Y., Anderson, R., Silver, J., Gregersen, P. K., & Karr, R. W. (1988). Analysis of the molecular specificities of anti-class II monoclonal antibodies by using L cell transfectants expressing HLA class II molecules. Journal of Immunology (Baltimore, Md. : 1950), 141(6), 2158-64.
Klohe EP, et al. Analysis of the Molecular Specificities of Anti-class II Monoclonal Antibodies By Using L Cell Transfectants Expressing HLA Class II Molecules. J Immunol. 1988 Sep 15;141(6):2158-64. PubMed PMID: 2459201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of the molecular specificities of anti-class II monoclonal antibodies by using L cell transfectants expressing HLA class II molecules. AU - Klohe,E P, AU - Watts,R, AU - Bahl,M, AU - Alber,C, AU - Yu,W Y, AU - Anderson,R, AU - Silver,J, AU - Gregersen,P K, AU - Karr,R W, PY - 1988/9/15/pubmed PY - 1988/9/15/medline PY - 1988/9/15/entrez SP - 2158 EP - 64 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 141 IS - 6 N2 - Expressible HLA class II alpha- and beta-chain cDNA were used for DNA-mediated gene transfer to produce L cell transfectants expressing single types of human class II molecules. Cloned transfectants expressing nine different class II molecules were isolated: DR alpha: DR1 beta I, DR alpha: DR4 beta I, DR alpha: DR5 beta I, DR alpha: DR5 beta III (DRw52), DR alpha: DR7 beta I, DR alpha: DR4/7 beta IV (DRw53), DQ7 alpha: DQw2 beta, DQ7 alpha: DQw3 beta, and DPw4 alpha: DPw4 beta. These class II-expressing transfectants were used to analyze by flow cytometry the molecular specificities of 20 anti-class II mAb. These analyes indicate that some mAb are more broadly reactive than was previously thought based on immunochemical studies. In contrast, the narrow molecular specificities of other anti-class II mAb were confirmed by this approach. Transfectants expressing human class II molecules should be valuable reagents for studies of B cell and T cell defined epitopes on these molecules. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2459201/Analysis_of_the_molecular_specificities_of_anti_class_II_monoclonal_antibodies_by_using_L_cell_transfectants_expressing_HLA_class_II_molecules_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2459201 DB - PRIME DP - Unbound Medicine ER -