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Therapeutic effects of mouse adipose-derived stem cells and losartan in the skeletal muscle of injured mdx mice.
Cell Transplant. 2015; 24(5):939-53.CT

Abstract

Duchenne muscular dystrophy (DMD) is an X-linked genetic disorder caused by mutations in the dystrophin gene. Adipose-derived stem cells (ASCs) are an attractive source of cells for stem cell therapy. Losartan has been reported to improve ASC transplantation in injured mouse muscles. In the present study, we investigated whether the combined treatment of losartan and ASCs in the injured muscles of mdx mice improves regeneration. The combined treatment of ASCs and losartan remarkably improved muscle regeneration and induced muscle hypertrophy. In addition, ASCs and losartan treatment downregulated transforming growth factor-β and inhibited muscle fibrosis. We observed cells coexpressing green fluorescent protein (GFP) and dystrophin in the muscle samples of mice transplanted with GFP-positive ASCs. In the coculture in vitro experiment, we also observed that the GFP ASCs differentiated into dystrophin-expressing myotubes. The present study shows that the combination of transplanted ASCs and treatment with losartan ameliorated muscle fibrosis and improved muscle regeneration in injured mdx mice. Thus, we suggest that combined treatment with losartan and ASCs could help to improve muscle regeneration in the muscles of injured patients, including DMD patients.

Authors+Show Affiliations

College of Veterinary Medicine, Kyungpook National University, Daegu City, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24593934

Citation

Lee, Eun-Mi, et al. "Therapeutic Effects of Mouse Adipose-derived Stem Cells and Losartan in the Skeletal Muscle of Injured Mdx Mice." Cell Transplantation, vol. 24, no. 5, 2015, pp. 939-53.
Lee EM, Kim AY, Lee EJ, et al. Therapeutic effects of mouse adipose-derived stem cells and losartan in the skeletal muscle of injured mdx mice. Cell Transplant. 2015;24(5):939-53.
Lee, E. M., Kim, A. Y., Lee, E. J., Park, J. K., Lee, M. M., Hwang, M., Kim, C. Y., Kim, S. Y., & Jeong, K. S. (2015). Therapeutic effects of mouse adipose-derived stem cells and losartan in the skeletal muscle of injured mdx mice. Cell Transplantation, 24(5), 939-53. https://doi.org/10.3727/096368914X678599
Lee EM, et al. Therapeutic Effects of Mouse Adipose-derived Stem Cells and Losartan in the Skeletal Muscle of Injured Mdx Mice. Cell Transplant. 2015;24(5):939-53. PubMed PMID: 24593934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Therapeutic effects of mouse adipose-derived stem cells and losartan in the skeletal muscle of injured mdx mice. AU - Lee,Eun-Mi, AU - Kim,Ah-Young, AU - Lee,Eun-Joo, AU - Park,Jin-Kyu, AU - Lee,Myeong-Mi, AU - Hwang,Meeyul, AU - Kim,Choong-Yong, AU - Kim,Shin-Yoon, AU - Jeong,Kyu-Shik, Y1 - 2014/03/03/ PY - 2014/3/6/entrez PY - 2014/3/7/pubmed PY - 2016/1/26/medline SP - 939 EP - 53 JF - Cell transplantation JO - Cell Transplant VL - 24 IS - 5 N2 - Duchenne muscular dystrophy (DMD) is an X-linked genetic disorder caused by mutations in the dystrophin gene. Adipose-derived stem cells (ASCs) are an attractive source of cells for stem cell therapy. Losartan has been reported to improve ASC transplantation in injured mouse muscles. In the present study, we investigated whether the combined treatment of losartan and ASCs in the injured muscles of mdx mice improves regeneration. The combined treatment of ASCs and losartan remarkably improved muscle regeneration and induced muscle hypertrophy. In addition, ASCs and losartan treatment downregulated transforming growth factor-β and inhibited muscle fibrosis. We observed cells coexpressing green fluorescent protein (GFP) and dystrophin in the muscle samples of mice transplanted with GFP-positive ASCs. In the coculture in vitro experiment, we also observed that the GFP ASCs differentiated into dystrophin-expressing myotubes. The present study shows that the combination of transplanted ASCs and treatment with losartan ameliorated muscle fibrosis and improved muscle regeneration in injured mdx mice. Thus, we suggest that combined treatment with losartan and ASCs could help to improve muscle regeneration in the muscles of injured patients, including DMD patients. SN - 1555-3892 UR - https://www.unboundmedicine.com/medline/citation/24593934/Therapeutic_effects_of_mouse_adipose_derived_stem_cells_and_losartan_in_the_skeletal_muscle_of_injured_mdx_mice_ L2 - http://journals.sagepub.com/doi/full/10.3727/096368914X678599?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -