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A comparative study of the molecular composition and electrophysiological activity of the venoms from two fishing spiders Dolomedes mizhoanus and Dolomedes sulfurous.
Toxicon. 2014 Jun; 83:35-42.T

Abstract

Dolomedes mizhoanus and Dolomedes sulfurous are two venomous spiders found in the same area in southern China and are characterized by living in water plants and feeding on fish. In this study, the chemical compositions and activities of these venoms were compared. Both venoms contain hundreds of peptides as shown by off-line RP-HPLC/MALDI-TOF-MS analysis, but have a different peptide distribution, with D. mizhoanus venom containing fewer high molecular mass (7000-9000 Da) peptides (3%) than D. sulfurous venom (25.6%). Patch-clamp analyses showed that both venoms inhibited voltage-activated Na(+), K(+) and Ca(2+) channels in rat DRG neurons, however, differences in their inhibitory effects were observed. In general, D. mizhoanus venom had lower inhibitory activity than D. sulfurous venom and both venoms had a different inhibitory spectrum against these ion channels, showing that both venoms are useful for identifying antagonists to them. In addition, intrathoracic injection of both venoms caused severe neurotoxic effects in zebrafish and death at higher concentrations, respectively. Considering that both spiders belong to the same genus, live in the same area and have similar habits, elucidation of the differences between the peptide toxins from both venoms would provide new molecular insights into the evolution of spider peptides.

Authors+Show Affiliations

Cooperative Innovation Center of Engineering and New Products for Developmental Biology, College of Life Sciences, Hunan Normal University, Changsha 410081, PR China.Cooperative Innovation Center of Engineering and New Products for Developmental Biology, College of Life Sciences, Hunan Normal University, Changsha 410081, PR China.Cooperative Innovation Center of Engineering and New Products for Developmental Biology, College of Life Sciences, Hunan Normal University, Changsha 410081, PR China.Cooperative Innovation Center of Engineering and New Products for Developmental Biology, College of Life Sciences, Hunan Normal University, Changsha 410081, PR China.Cooperative Innovation Center of Engineering and New Products for Developmental Biology, College of Life Sciences, Hunan Normal University, Changsha 410081, PR China.Cooperative Innovation Center of Engineering and New Products for Developmental Biology, College of Life Sciences, Hunan Normal University, Changsha 410081, PR China. Electronic address: liuzh@hunnu.edu.cn.Cooperative Innovation Center of Engineering and New Products for Developmental Biology, College of Life Sciences, Hunan Normal University, Changsha 410081, PR China. Electronic address: liangsp@hunnu.edu.cn.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24593962

Citation

Li, Jiayan, et al. "A Comparative Study of the Molecular Composition and Electrophysiological Activity of the Venoms From Two Fishing Spiders Dolomedes Mizhoanus and Dolomedes Sulfurous." Toxicon : Official Journal of the International Society On Toxinology, vol. 83, 2014, pp. 35-42.
Li J, Li D, Zhang F, et al. A comparative study of the molecular composition and electrophysiological activity of the venoms from two fishing spiders Dolomedes mizhoanus and Dolomedes sulfurous. Toxicon. 2014;83:35-42.
Li, J., Li, D., Zhang, F., Wang, H., Yu, H., Liu, Z., & Liang, S. (2014). A comparative study of the molecular composition and electrophysiological activity of the venoms from two fishing spiders Dolomedes mizhoanus and Dolomedes sulfurous. Toxicon : Official Journal of the International Society On Toxinology, 83, 35-42. https://doi.org/10.1016/j.toxicon.2014.02.014
Li J, et al. A Comparative Study of the Molecular Composition and Electrophysiological Activity of the Venoms From Two Fishing Spiders Dolomedes Mizhoanus and Dolomedes Sulfurous. Toxicon. 2014;83:35-42. PubMed PMID: 24593962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparative study of the molecular composition and electrophysiological activity of the venoms from two fishing spiders Dolomedes mizhoanus and Dolomedes sulfurous. AU - Li,Jiayan, AU - Li,Dan, AU - Zhang,Fan, AU - Wang,Hengyun, AU - Yu,Hai, AU - Liu,Zhonghua, AU - Liang,Songping, Y1 - 2014/03/02/ PY - 2013/11/15/received PY - 2014/02/11/revised PY - 2014/02/20/accepted PY - 2014/3/6/entrez PY - 2014/3/7/pubmed PY - 2015/1/13/medline KW - Dolomedes mizhoanus KW - Dolomedes sulfurous KW - MALDI-TOF-MS KW - RP-HPLC KW - Spider venom KW - Voltage-activated ion channels SP - 35 EP - 42 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 83 N2 - Dolomedes mizhoanus and Dolomedes sulfurous are two venomous spiders found in the same area in southern China and are characterized by living in water plants and feeding on fish. In this study, the chemical compositions and activities of these venoms were compared. Both venoms contain hundreds of peptides as shown by off-line RP-HPLC/MALDI-TOF-MS analysis, but have a different peptide distribution, with D. mizhoanus venom containing fewer high molecular mass (7000-9000 Da) peptides (3%) than D. sulfurous venom (25.6%). Patch-clamp analyses showed that both venoms inhibited voltage-activated Na(+), K(+) and Ca(2+) channels in rat DRG neurons, however, differences in their inhibitory effects were observed. In general, D. mizhoanus venom had lower inhibitory activity than D. sulfurous venom and both venoms had a different inhibitory spectrum against these ion channels, showing that both venoms are useful for identifying antagonists to them. In addition, intrathoracic injection of both venoms caused severe neurotoxic effects in zebrafish and death at higher concentrations, respectively. Considering that both spiders belong to the same genus, live in the same area and have similar habits, elucidation of the differences between the peptide toxins from both venoms would provide new molecular insights into the evolution of spider peptides. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/24593962/A_comparative_study_of_the_molecular_composition_and_electrophysiological_activity_of_the_venoms_from_two_fishing_spiders_Dolomedes_mizhoanus_and_Dolomedes_sulfurous_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(14)00058-0 DB - PRIME DP - Unbound Medicine ER -