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Are there any different effects of Bifidobacterium, Lactobacillus and Streptococcus on intestinal sensation, barrier function and intestinal immunity in PI-IBS mouse model?
PLoS One. 2014; 9(3):e90153.Plos

Abstract

BACKGROUND AND AIMS

Research has increasingly suggested that gut flora plays an important role in the development of post-infectious irritable bowel syndrome (PI-IBS). Studies of the curative effect of probiotics for IBS have usually been positive but not always. However, the differences of treatment effects and mechanisms among probiotic stains, or mixture of them, are not clear. In this study, we compared the effects of different probiotics (Befidobacterium, Lactobacillus, Streptococcus or mixture of the three) on intestinal sensation, barrier function and intestinal immunity in PI-IBS mouse model.

METHODS

PI-IBS model was induced by Trichinella spiralis infection in mice. Different probiotics were administered to mice after 8 weeks infection. Visceral sensitivity was measured by scores of abdominal withdrawal reflex (AWR) and the threshold intensity of colorectal distention. Colonic smooth muscle contractile response was assessed by contraction of the longitudinal muscle strips. Plasma diamine oxidase (DAO) and d-lactate were determined by an enzymatic spectrophotometry. Expression of tight junction proteins and cytokines in ileum were measured by Western blotting.

RESULTS

Compared to control mice, PI-IBS mice treated either alone with Befidobacterium or Lactobacillus (but not Streptococcus), or the mixture of the three exhibited not only decreased AWR score and contractile response, but also reduced plasma DAO and D-lactate. These probiotic treatments also suppressed the expression of proinflammatory cytokine IL-6 and IL-17 and promoted the expression of major tight junction proteins claudin-1 and occludin. The mixture of the three probiotic strains performed better than the individual in up-regulating these tight junction proteins and suppressing IL-17 expression.

CONCLUSIONS

Bifidobacterium and Lactobacillus, but not Streptococcus, alleviated visceral hypersensitivity and recovered intestinal barrier function as well as inflammation in PI-IBS mouse model, which correlated with an increase of major tight junction proteins. In addition, Mixture of three species was indicated to be superior to a single one.

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Authors+Show Affiliations

Wang H
Division of Gastroenterology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Gong J
Division of Gastroenterology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Wang W
Division of Gastroenterology, Hospital of Dongfeng Motor Company of Hubei University of Medicine, Shiyan, Hubei, China.
Long Y
Division of Gastroenterology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Fu X
Division of Culture Collection, Hubei Center of Industrial Culture Collection and Research, Wuhan, Hubei, China.
Fu Y
Division of Gastroenterology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Qian W
Division of Gastroenterology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Hou X
Division of Gastroenterology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

MeSH

AnimalsBifidobacteriumBlotting, WesternCytokinesIntestinal MucosaIntestinesLactobacillusMicePermeabilityProbioticsSpecies SpecificityStreptococcus

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24595218

Citation

Wang, Huan, et al. "Are There Any Different Effects of Bifidobacterium, Lactobacillus and Streptococcus On Intestinal Sensation, Barrier Function and Intestinal Immunity in PI-IBS Mouse Model?" PloS One, vol. 9, no. 3, 2014, pp. e90153.
Wang H, Gong J, Wang W, et al. Are there any different effects of Bifidobacterium, Lactobacillus and Streptococcus on intestinal sensation, barrier function and intestinal immunity in PI-IBS mouse model? PLoS One. 2014;9(3):e90153.
Wang, H., Gong, J., Wang, W., Long, Y., Fu, X., Fu, Y., Qian, W., & Hou, X. (2014). Are there any different effects of Bifidobacterium, Lactobacillus and Streptococcus on intestinal sensation, barrier function and intestinal immunity in PI-IBS mouse model? PloS One, 9(3), e90153. https://doi.org/10.1371/journal.pone.0090153
Wang H, et al. Are There Any Different Effects of Bifidobacterium, Lactobacillus and Streptococcus On Intestinal Sensation, Barrier Function and Intestinal Immunity in PI-IBS Mouse Model. PLoS One. 2014;9(3):e90153. PubMed PMID: 24595218.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Are there any different effects of Bifidobacterium, Lactobacillus and Streptococcus on intestinal sensation, barrier function and intestinal immunity in PI-IBS mouse model? AU - Wang,Huan, AU - Gong,Jing, AU - Wang,Wenfeng, AU - Long,Yanqin, AU - Fu,Xiaochao, AU - Fu,Yu, AU - Qian,Wei, AU - Hou,Xiaohua, Y1 - 2014/03/03/ PY - 2013/10/13/received PY - 2014/01/27/accepted PY - 2014/3/6/entrez PY - 2014/3/7/pubmed PY - 2015/1/16/medline SP - e90153 EP - e90153 JF - PloS one JO - PLoS One VL - 9 IS - 3 N2 - BACKGROUND AND AIMS: Research has increasingly suggested that gut flora plays an important role in the development of post-infectious irritable bowel syndrome (PI-IBS). Studies of the curative effect of probiotics for IBS have usually been positive but not always. However, the differences of treatment effects and mechanisms among probiotic stains, or mixture of them, are not clear. In this study, we compared the effects of different probiotics (Befidobacterium, Lactobacillus, Streptococcus or mixture of the three) on intestinal sensation, barrier function and intestinal immunity in PI-IBS mouse model. METHODS: PI-IBS model was induced by Trichinella spiralis infection in mice. Different probiotics were administered to mice after 8 weeks infection. Visceral sensitivity was measured by scores of abdominal withdrawal reflex (AWR) and the threshold intensity of colorectal distention. Colonic smooth muscle contractile response was assessed by contraction of the longitudinal muscle strips. Plasma diamine oxidase (DAO) and d-lactate were determined by an enzymatic spectrophotometry. Expression of tight junction proteins and cytokines in ileum were measured by Western blotting. RESULTS: Compared to control mice, PI-IBS mice treated either alone with Befidobacterium or Lactobacillus (but not Streptococcus), or the mixture of the three exhibited not only decreased AWR score and contractile response, but also reduced plasma DAO and D-lactate. These probiotic treatments also suppressed the expression of proinflammatory cytokine IL-6 and IL-17 and promoted the expression of major tight junction proteins claudin-1 and occludin. The mixture of the three probiotic strains performed better than the individual in up-regulating these tight junction proteins and suppressing IL-17 expression. CONCLUSIONS: Bifidobacterium and Lactobacillus, but not Streptococcus, alleviated visceral hypersensitivity and recovered intestinal barrier function as well as inflammation in PI-IBS mouse model, which correlated with an increase of major tight junction proteins. In addition, Mixture of three species was indicated to be superior to a single one. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24595218/Are_there_any_different_effects_of_Bifidobacterium_Lactobacillus_and_Streptococcus_on_intestinal_sensation_barrier_function_and_intestinal_immunity_in_PI_IBS_mouse_model DB - PRIME DP - Unbound Medicine ER -