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Genetic rearrangements of six wheat-agropyron cristatum 6P addition lines revealed by molecular markers.
PLoS One. 2014; 9(3):e91066.Plos

Abstract

Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP) not only is cultivated as pasture fodder but also could provide many desirable genes for wheat improvement. It is critical to obtain common wheat-A. cristatum alien disomic addition lines to locate the desired genes on the P genome chromosomes. Comparative analysis of the homoeologous relationships between the P genome chromosome and wheat genome chromosomes is a key step in transferring different desirable genes into common wheat and producing the desired alien translocation line while compensating for the loss of wheat chromatin. In this study, six common wheat-A. cristatum disomic addition lines were produced and analyzed by phenotypic examination, genomic in situ hybridization (GISH), SSR markers from the ABD genomes and STS markers from the P genome. Comparative maps, six in total, were generated and demonstrated that all six addition lines belonged to homoeologous group 6. However, chromosome 6P had undergone obvious rearrangements in different addition lines compared with the wheat chromosome, indicating that to obtain a genetic compensating alien translocation line, one should recombine alien chromosomal regions with homoeologous wheat chromosomes. Indeed, these addition lines were classified into four types based on the comparative mapping: 6PI, 6PII, 6PIII, and 6PIV. The different types of chromosome 6P possessed different desirable genes. For example, the 6PI type, containing three addition lines, carried genes conferring high numbers of kernels per spike and resistance to powdery mildew, important traits for wheat improvement. These results may prove valuable for promoting the development of conventional chromosome engineering techniques toward molecular chromosome engineering.

Authors+Show Affiliations

National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24595330

Citation

Han, Haiming, et al. "Genetic Rearrangements of Six Wheat-agropyron Cristatum 6P Addition Lines Revealed By Molecular Markers." PloS One, vol. 9, no. 3, 2014, pp. e91066.
Han H, Bai L, Su J, et al. Genetic rearrangements of six wheat-agropyron cristatum 6P addition lines revealed by molecular markers. PLoS One. 2014;9(3):e91066.
Han, H., Bai, L., Su, J., Zhang, J., Song, L., Gao, A., Yang, X., Li, X., Liu, W., & Li, L. (2014). Genetic rearrangements of six wheat-agropyron cristatum 6P addition lines revealed by molecular markers. PloS One, 9(3), e91066. https://doi.org/10.1371/journal.pone.0091066
Han H, et al. Genetic Rearrangements of Six Wheat-agropyron Cristatum 6P Addition Lines Revealed By Molecular Markers. PLoS One. 2014;9(3):e91066. PubMed PMID: 24595330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic rearrangements of six wheat-agropyron cristatum 6P addition lines revealed by molecular markers. AU - Han,Haiming, AU - Bai,Li, AU - Su,Junji, AU - Zhang,Jinpeng, AU - Song,Liqiang, AU - Gao,Ainong, AU - Yang,Xinming, AU - Li,Xiuquan, AU - Liu,Weihua, AU - Li,Lihui, Y1 - 2014/03/04/ PY - 2013/08/11/received PY - 2014/02/07/accepted PY - 2014/3/6/entrez PY - 2014/3/7/pubmed PY - 2014/11/5/medline SP - e91066 EP - e91066 JF - PloS one JO - PLoS One VL - 9 IS - 3 N2 - Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP) not only is cultivated as pasture fodder but also could provide many desirable genes for wheat improvement. It is critical to obtain common wheat-A. cristatum alien disomic addition lines to locate the desired genes on the P genome chromosomes. Comparative analysis of the homoeologous relationships between the P genome chromosome and wheat genome chromosomes is a key step in transferring different desirable genes into common wheat and producing the desired alien translocation line while compensating for the loss of wheat chromatin. In this study, six common wheat-A. cristatum disomic addition lines were produced and analyzed by phenotypic examination, genomic in situ hybridization (GISH), SSR markers from the ABD genomes and STS markers from the P genome. Comparative maps, six in total, were generated and demonstrated that all six addition lines belonged to homoeologous group 6. However, chromosome 6P had undergone obvious rearrangements in different addition lines compared with the wheat chromosome, indicating that to obtain a genetic compensating alien translocation line, one should recombine alien chromosomal regions with homoeologous wheat chromosomes. Indeed, these addition lines were classified into four types based on the comparative mapping: 6PI, 6PII, 6PIII, and 6PIV. The different types of chromosome 6P possessed different desirable genes. For example, the 6PI type, containing three addition lines, carried genes conferring high numbers of kernels per spike and resistance to powdery mildew, important traits for wheat improvement. These results may prove valuable for promoting the development of conventional chromosome engineering techniques toward molecular chromosome engineering. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24595330/Genetic_rearrangements_of_six_wheat_agropyron_cristatum_6P_addition_lines_revealed_by_molecular_markers_ L2 - https://dx.plos.org/10.1371/journal.pone.0091066 DB - PRIME DP - Unbound Medicine ER -