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Possible role of GABAergic depolarization in neocortical neurons in generating hyperexcitatory behaviors during emergence from sevoflurane anesthesia in the rat.
ASN Neuro. 2014 Apr 04; 6(2)AN

Abstract

Hyperexcitatory behaviors occurring after sevoflurane anesthesia are of serious clinical concern, but the underlying mechanism is unknown. These behaviors may result from the potentiation by sevoflurane of GABAergic depolarization/excitation in neocortical neurons, cells implicated in the genesis of consciousness and arousal. The current study sought to provide evidence for this hypothesis with rats, the neocortical neurons of which are known to respond to GABA (γ-aminobutyric acid) with depolarization/excitation at early stages of development (i.e., until the second postnatal week) and with hyperpolarization/inhibition during adulthood. Employing behavioral tests and electrophysiological recordings in neocortical slice preparations, we found: (1) sevoflurane produced PAHBs (post-anesthetic hyperexcitatory behaviors) in postnatal day (P)1-15 rats, whereas it failed to elicit PAHBs in P16 or older rats; (2) GABAergic PSPs (postsynaptic potentials) were depolarizing/excitatory in the neocortical neurons of P5 and P10 rats, whereas mostly hyperpolarizing/inhibitory in the cells of adult rats; (3) at P14-15, <50% of rats had PAHBs and, in general, the cells of the animals with PAHBs exhibited strongly depolarizing GABAergic PSPs, whereas those without PAHBs showed hyperpolarizing or weakly depolarizing GABAergic PSPs; (4) bumetanide [inhibitor of the Cl- importer NKCC (Na+-K+-2Cl- cotransporter)] treatment at P5 suppressed PAHBs and depolarizing GABAergic responses; and (5) sevoflurane at 1% (i.e., concentration<1 minimum alveolar concentration) potentiated depolarizing GABAergic PSPs in the neurons of P5 and P10 rats and of P14-15 animals with PAHBs, evoking action potentials in ≥50% of these cells. On the basis of these results, we conclude that sevoflurane may produce PAHBs by potentiating GABAergic depolarization/excitation in neocortical neurons.

Authors+Show Affiliations

*Department of Anesthesiology and Pain Medicine, Korea University College of Medicine, Guro Hospital, 148 Gurodong-ro, Guro-gu, Seoul, Korea.†Department of Anesthesiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 20092, China.‡Department of Physiology and Neuroscience Research Institute, Korea University College of Medicine, 126-1 Anam-dong 5-ga, Seoul, Korea.‡Department of Physiology and Neuroscience Research Institute, Korea University College of Medicine, 126-1 Anam-dong 5-ga, Seoul, Korea.‡Department of Physiology and Neuroscience Research Institute, Korea University College of Medicine, 126-1 Anam-dong 5-ga, Seoul, Korea.‡Department of Physiology and Neuroscience Research Institute, Korea University College of Medicine, 126-1 Anam-dong 5-ga, Seoul, Korea.*Department of Anesthesiology and Pain Medicine, Korea University College of Medicine, Guro Hospital, 148 Gurodong-ro, Guro-gu, Seoul, Korea.*Department of Anesthesiology and Pain Medicine, Korea University College of Medicine, Guro Hospital, 148 Gurodong-ro, Guro-gu, Seoul, Korea.‡Department of Physiology and Neuroscience Research Institute, Korea University College of Medicine, 126-1 Anam-dong 5-ga, Seoul, Korea.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24597723

Citation

Lim, Byung-Gun, et al. "Possible Role of GABAergic Depolarization in Neocortical Neurons in Generating Hyperexcitatory Behaviors During Emergence From Sevoflurane Anesthesia in the Rat." ASN Neuro, vol. 6, no. 2, 2014.
Lim BG, Shen FY, Kim YB, et al. Possible role of GABAergic depolarization in neocortical neurons in generating hyperexcitatory behaviors during emergence from sevoflurane anesthesia in the rat. ASN Neuro. 2014;6(2).
Lim, B. G., Shen, F. Y., Kim, Y. B., Kim, W. B., Kim, Y. S., Han, H. C., Lee, M. K., Kong, M. H., & Kim, Y. I. (2014). Possible role of GABAergic depolarization in neocortical neurons in generating hyperexcitatory behaviors during emergence from sevoflurane anesthesia in the rat. ASN Neuro, 6(2). https://doi.org/10.1042/AN20140004
Lim BG, et al. Possible Role of GABAergic Depolarization in Neocortical Neurons in Generating Hyperexcitatory Behaviors During Emergence From Sevoflurane Anesthesia in the Rat. ASN Neuro. 2014 Apr 4;6(2) PubMed PMID: 24597723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Possible role of GABAergic depolarization in neocortical neurons in generating hyperexcitatory behaviors during emergence from sevoflurane anesthesia in the rat. AU - Lim,Byung-Gun, AU - Shen,Feng-Yan, AU - Kim,Young-Beom, AU - Kim,Woong Bin, AU - Kim,Yoon Sik, AU - Han,Hee Chul, AU - Lee,Mi-Kyoung, AU - Kong,Myoung-Hoon, AU - Kim,Yang In, Y1 - 2014/04/04/ PY - 2014/3/7/entrez PY - 2014/3/7/pubmed PY - 2014/12/15/medline JF - ASN neuro JO - ASN Neuro VL - 6 IS - 2 N2 - Hyperexcitatory behaviors occurring after sevoflurane anesthesia are of serious clinical concern, but the underlying mechanism is unknown. These behaviors may result from the potentiation by sevoflurane of GABAergic depolarization/excitation in neocortical neurons, cells implicated in the genesis of consciousness and arousal. The current study sought to provide evidence for this hypothesis with rats, the neocortical neurons of which are known to respond to GABA (γ-aminobutyric acid) with depolarization/excitation at early stages of development (i.e., until the second postnatal week) and with hyperpolarization/inhibition during adulthood. Employing behavioral tests and electrophysiological recordings in neocortical slice preparations, we found: (1) sevoflurane produced PAHBs (post-anesthetic hyperexcitatory behaviors) in postnatal day (P)1-15 rats, whereas it failed to elicit PAHBs in P16 or older rats; (2) GABAergic PSPs (postsynaptic potentials) were depolarizing/excitatory in the neocortical neurons of P5 and P10 rats, whereas mostly hyperpolarizing/inhibitory in the cells of adult rats; (3) at P14-15, <50% of rats had PAHBs and, in general, the cells of the animals with PAHBs exhibited strongly depolarizing GABAergic PSPs, whereas those without PAHBs showed hyperpolarizing or weakly depolarizing GABAergic PSPs; (4) bumetanide [inhibitor of the Cl- importer NKCC (Na+-K+-2Cl- cotransporter)] treatment at P5 suppressed PAHBs and depolarizing GABAergic responses; and (5) sevoflurane at 1% (i.e., concentration<1 minimum alveolar concentration) potentiated depolarizing GABAergic PSPs in the neurons of P5 and P10 rats and of P14-15 animals with PAHBs, evoking action potentials in ≥50% of these cells. On the basis of these results, we conclude that sevoflurane may produce PAHBs by potentiating GABAergic depolarization/excitation in neocortical neurons. SN - 1759-0914 UR - https://www.unboundmedicine.com/medline/citation/24597723/Possible_role_of_GABAergic_depolarization_in_neocortical_neurons_in_generating_hyperexcitatory_behaviors_during_emergence_from_sevoflurane_anesthesia_in_the_rat_ L2 - http://journals.sagepub.com/doi/full/10.1042/AN20140004?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -