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Novel influenza virus vectors expressing Brucella L7/L12 or Omp16 proteins in cattle induced a strong T-cell immune response, as well as high protectiveness against B. abortus infection.
Vaccine. 2014 Apr 11; 32(18):2034-41.V

Abstract

This paper presents the results of a study of the immunogenicity and protectiveness of new candidate vector vaccine against Brucella abortus - a bivalent vaccine formulation consisting of a mixture of recombinant influenza A subtype H5N1 or H1N1 (viral constructs vaccine formulation) viruses expressing Brucella ribosomal protein L7/L12 and Omp16, in cattle. To increase the effectiveness of the candidate vaccine, adjuvants such as Montanide Gel01 or chitosan were included in its composition. Immunization of cattle (heifers aged 1-1.5 years, 5 animals per group) with the viral constructs vaccine formulation only, or its combination with adjuvants Montanide Gel01 or chitosan, was conducted via the conjunctival method using cross prime (influenza virus subtype H5N1) and booster (influenza virus subtype H1N1) vaccination schedules at an interval of 28 days. Vaccine candidates were evaluated in comparison with the positive (B. abortus S19) and negative (PBS) controls. The viral constructs vaccine formulations, particularly in combination with Montanide Gel01 adjuvant promoted formation of IgG antibodies (with a predominance of antibodies of isotype IgG2a) against Brucella L7/L12 and Omp16 proteins in ELISA. Moreover, these vaccines in cattle induced a strong antigen-specific T-cell immune response, as indicated by a high number of CD4(+) and CD8(+) cells, as well as the concentration of IFN-γ, and most importantly provided a high level of protectiveness comparable to the commercial B. abortus S19 vaccine and superior to the B. abortus S19 vaccine in combination with Montanide Gel01 adjuvant. Based on these findings, we recommended the bivalent vaccine formulation containing the adjuvant Montanide Gel01 for practical use in cattle.

Authors+Show Affiliations

The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan. Electronic address: tabynov_81@mail.ru.The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.The Research Institute for Biological Safety Problems, 080409, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Kazakhstan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24598723

Citation

Tabynov, Kaissar, et al. "Novel Influenza Virus Vectors Expressing Brucella L7/L12 or Omp16 Proteins in Cattle Induced a Strong T-cell Immune Response, as Well as High Protectiveness Against B. Abortus Infection." Vaccine, vol. 32, no. 18, 2014, pp. 2034-41.
Tabynov K, Kydyrbayev Z, Ryskeldinova S, et al. Novel influenza virus vectors expressing Brucella L7/L12 or Omp16 proteins in cattle induced a strong T-cell immune response, as well as high protectiveness against B. abortus infection. Vaccine. 2014;32(18):2034-41.
Tabynov, K., Kydyrbayev, Z., Ryskeldinova, S., Yespembetov, B., Zinina, N., Assanzhanova, N., Kozhamkulov, Y., Inkarbekov, D., Gotskina, T., & Sansyzbay, A. (2014). Novel influenza virus vectors expressing Brucella L7/L12 or Omp16 proteins in cattle induced a strong T-cell immune response, as well as high protectiveness against B. abortus infection. Vaccine, 32(18), 2034-41. https://doi.org/10.1016/j.vaccine.2014.02.058
Tabynov K, et al. Novel Influenza Virus Vectors Expressing Brucella L7/L12 or Omp16 Proteins in Cattle Induced a Strong T-cell Immune Response, as Well as High Protectiveness Against B. Abortus Infection. Vaccine. 2014 Apr 11;32(18):2034-41. PubMed PMID: 24598723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel influenza virus vectors expressing Brucella L7/L12 or Omp16 proteins in cattle induced a strong T-cell immune response, as well as high protectiveness against B. abortus infection. AU - Tabynov,Kaissar, AU - Kydyrbayev,Zhailaubay, AU - Ryskeldinova,Sholpan, AU - Yespembetov,Bolat, AU - Zinina,Nadezhda, AU - Assanzhanova,Nurika, AU - Kozhamkulov,Yerken, AU - Inkarbekov,Dulat, AU - Gotskina,Tatyana, AU - Sansyzbay,Abylai, Y1 - 2014/03/02/ PY - 2014/01/04/received PY - 2014/02/04/revised PY - 2014/02/12/accepted PY - 2014/3/7/entrez PY - 2014/3/7/pubmed PY - 2014/8/26/medline KW - Brucella abortus KW - Cattle KW - Influenza virus KW - Protectiveness KW - T-cell immune response KW - Vector vaccine SP - 2034 EP - 41 JF - Vaccine JO - Vaccine VL - 32 IS - 18 N2 - This paper presents the results of a study of the immunogenicity and protectiveness of new candidate vector vaccine against Brucella abortus - a bivalent vaccine formulation consisting of a mixture of recombinant influenza A subtype H5N1 or H1N1 (viral constructs vaccine formulation) viruses expressing Brucella ribosomal protein L7/L12 and Omp16, in cattle. To increase the effectiveness of the candidate vaccine, adjuvants such as Montanide Gel01 or chitosan were included in its composition. Immunization of cattle (heifers aged 1-1.5 years, 5 animals per group) with the viral constructs vaccine formulation only, or its combination with adjuvants Montanide Gel01 or chitosan, was conducted via the conjunctival method using cross prime (influenza virus subtype H5N1) and booster (influenza virus subtype H1N1) vaccination schedules at an interval of 28 days. Vaccine candidates were evaluated in comparison with the positive (B. abortus S19) and negative (PBS) controls. The viral constructs vaccine formulations, particularly in combination with Montanide Gel01 adjuvant promoted formation of IgG antibodies (with a predominance of antibodies of isotype IgG2a) against Brucella L7/L12 and Omp16 proteins in ELISA. Moreover, these vaccines in cattle induced a strong antigen-specific T-cell immune response, as indicated by a high number of CD4(+) and CD8(+) cells, as well as the concentration of IFN-γ, and most importantly provided a high level of protectiveness comparable to the commercial B. abortus S19 vaccine and superior to the B. abortus S19 vaccine in combination with Montanide Gel01 adjuvant. Based on these findings, we recommended the bivalent vaccine formulation containing the adjuvant Montanide Gel01 for practical use in cattle. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/24598723/Novel_influenza_virus_vectors_expressing_Brucella_L7/L12_or_Omp16_proteins_in_cattle_induced_a_strong_T_cell_immune_response_as_well_as_high_protectiveness_against_B__abortus_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(14)00243-6 DB - PRIME DP - Unbound Medicine ER -