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Systematic screening of plant extracts from the Brazilian Pantanal with antimicrobial activity against bacteria with cariogenic relevance.
Caries Res 2014; 48(5):353-60CR

Abstract

This study proposes a bioprospection methodology regarding the antimicrobial potential of plant extracts against bacteria with cariogenic relevance. Sixty extracts were obtained from ten plants--(1) Jatropha weddelliana, (2) Attalea phalerata, (3) Buchenavia tomentosa, (4) Croton doctoris, (5) Mouriri elliptica, (6) Mascagnia benthamiana, (7) Senna aculeata, (8) Unonopsis guatterioides, (9) Allagoptera leucocalyx and (10) Bactris glaucescens--using different extraction methods - (A) 70° ethanol 72 h/25°C, (B) water 5 min/100°C, (C) water 1 h/55°C, (D) water 72 h/25°C, (E) hexane 72 h/25°C and (F) 90° ethanol 72 h/25°C. The plants were screened for antibacterial activity at 50 mg/ml using the agar well diffusion test against Actinomyces naeslundii ATCC 19039, Lactobacillus acidophilus ATCC 4356, Streptococcus gordonii ATCC 10558, Streptococcus mutans ATCC 35688, Streptococcus sanguinis ATCC 10556, Streptococcus sobrinus ATCC 33478 and Streptococcus mitis ATCC 9811. The active extracts were tested to determine their minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), cytotoxicity and chemical characterization. Forty-seven extracts (78%) were active against at least one microorganism. Extract 4A demonstrated the lowest MIC and MBC for all microorganisms except S. gordonii and the extract at MIC concentration was non-cytotoxic. The concentrated extracts were slightly cytotoxic. Electrospray ionization with tandem mass spectrometry analyses demonstrated that the extract constituents coincided with the mass of the terpenoids and phenolics. Overall, the best results were obtained for extraction methods A, B and C. The present work proved the antimicrobial activity of several plants. Particularly, extracts from C. doctoris were the most active against bacteria involved in dental caries disease.

Authors+Show Affiliations

Department of Biosciences and Oral Diagnosis, Universidade Estadual Paulista (UNESP), São José dos Campos, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24603299

Citation

Brighenti, F L., et al. "Systematic Screening of Plant Extracts From the Brazilian Pantanal With Antimicrobial Activity Against Bacteria With Cariogenic Relevance." Caries Research, vol. 48, no. 5, 2014, pp. 353-60.
Brighenti FL, Salvador MJ, Delbem AC, et al. Systematic screening of plant extracts from the Brazilian Pantanal with antimicrobial activity against bacteria with cariogenic relevance. Caries Res. 2014;48(5):353-60.
Brighenti, F. L., Salvador, M. J., Delbem, A. C., Delbem, Á. C., Oliveira, M. A., Soares, C. P., ... Koga-Ito, C. Y. (2014). Systematic screening of plant extracts from the Brazilian Pantanal with antimicrobial activity against bacteria with cariogenic relevance. Caries Research, 48(5), pp. 353-60. doi:10.1159/000357225.
Brighenti FL, et al. Systematic Screening of Plant Extracts From the Brazilian Pantanal With Antimicrobial Activity Against Bacteria With Cariogenic Relevance. Caries Res. 2014;48(5):353-60. PubMed PMID: 24603299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Systematic screening of plant extracts from the Brazilian Pantanal with antimicrobial activity against bacteria with cariogenic relevance. AU - Brighenti,F L, AU - Salvador,M J, AU - Delbem,Alberto Carlos Botazzo, AU - Delbem,Ádina Cleia Bottazzo, AU - Oliveira,M A C, AU - Soares,C P, AU - Freitas,L S F, AU - Koga-Ito,C Y, Y1 - 2014/03/06/ PY - 2013/05/20/received PY - 2013/11/12/accepted PY - 2014/3/8/entrez PY - 2014/3/8/pubmed PY - 2015/7/1/medline SP - 353 EP - 60 JF - Caries research JO - Caries Res. VL - 48 IS - 5 N2 - This study proposes a bioprospection methodology regarding the antimicrobial potential of plant extracts against bacteria with cariogenic relevance. Sixty extracts were obtained from ten plants--(1) Jatropha weddelliana, (2) Attalea phalerata, (3) Buchenavia tomentosa, (4) Croton doctoris, (5) Mouriri elliptica, (6) Mascagnia benthamiana, (7) Senna aculeata, (8) Unonopsis guatterioides, (9) Allagoptera leucocalyx and (10) Bactris glaucescens--using different extraction methods - (A) 70° ethanol 72 h/25°C, (B) water 5 min/100°C, (C) water 1 h/55°C, (D) water 72 h/25°C, (E) hexane 72 h/25°C and (F) 90° ethanol 72 h/25°C. The plants were screened for antibacterial activity at 50 mg/ml using the agar well diffusion test against Actinomyces naeslundii ATCC 19039, Lactobacillus acidophilus ATCC 4356, Streptococcus gordonii ATCC 10558, Streptococcus mutans ATCC 35688, Streptococcus sanguinis ATCC 10556, Streptococcus sobrinus ATCC 33478 and Streptococcus mitis ATCC 9811. The active extracts were tested to determine their minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), cytotoxicity and chemical characterization. Forty-seven extracts (78%) were active against at least one microorganism. Extract 4A demonstrated the lowest MIC and MBC for all microorganisms except S. gordonii and the extract at MIC concentration was non-cytotoxic. The concentrated extracts were slightly cytotoxic. Electrospray ionization with tandem mass spectrometry analyses demonstrated that the extract constituents coincided with the mass of the terpenoids and phenolics. Overall, the best results were obtained for extraction methods A, B and C. The present work proved the antimicrobial activity of several plants. Particularly, extracts from C. doctoris were the most active against bacteria involved in dental caries disease. SN - 1421-976X UR - https://www.unboundmedicine.com/medline/citation/24603299/Systematic_screening_of_plant_extracts_from_the_Brazilian_Pantanal_with_antimicrobial_activity_against_bacteria_with_cariogenic_relevance_ L2 - https://www.karger.com?DOI=10.1159/000357225 DB - PRIME DP - Unbound Medicine ER -