Tags

Type your tag names separated by a space and hit enter

Comparative Burkholderia pseudomallei natural history virulence studies using an aerosol murine model of infection.
Sci Rep. 2014 Mar 07; 4:4305.SR

Abstract

Melioidosis is an endemic disease caused by the bacterium Burkholderia pseudomallei. Concerns exist regarding B. pseudomallei use as a potential bio-threat agent causing persistent infections and typically manifesting as severe pneumonia capable of causing fatal bacteremia. Development of suitable therapeutics against melioidosis is complicated due to high degree of genetic and phenotypic variability among B. pseudomallei isolates and lack of data establishing commonly accepted strains for comparative studies. Further, the impact of strain variation on virulence, disease presentation, and mortality is not well understood. Therefore, this study evaluate and compare the virulence and disease progression of B. pseudomallei strains K96243 and HBPUB10303a, following aerosol challenge in a standardized BALB/c mouse model of infection. The natural history analysis of disease progression monitored conditions such as weight, body temperature, appearance, activity, bacteremia, organ and tissue colonization (pathological and histological analysis) and immunological responses. This study provides a detailed, direct comparison of infection with different B. pseudomallei strains and set up the basis for a standardized model useful to test different medical countermeasures against Burkholderia species. Further, this protocol serves as a guideline to standardize other bacterial aerosol models of infection or to define biomarkers of infectious processes caused by other intracellular pathogens.

Authors+Show Affiliations

Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555.1] Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555 [2] Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, 77555.Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555.Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555.1] Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555 [2] Department of Pathology, University of Texas Medical Branch, Galveston, TX, 77555.1] Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555 [2] Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, 77555.1] Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555 [2] Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, 77555.1] Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555 [2] Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, 77555.1] Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555 [2] Department of Pathology, University of Texas Medical Branch, Galveston, TX, 77555.1] Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555 [2] Department of Pathology, University of Texas Medical Branch, Galveston, TX, 77555.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24603493

Citation

Massey, Shane, et al. "Comparative Burkholderia Pseudomallei Natural History Virulence Studies Using an Aerosol Murine Model of Infection." Scientific Reports, vol. 4, 2014, p. 4305.
Massey S, Yeager LA, Blumentritt CA, et al. Comparative Burkholderia pseudomallei natural history virulence studies using an aerosol murine model of infection. Sci Rep. 2014;4:4305.
Massey, S., Yeager, L. A., Blumentritt, C. A., Vijayakumar, S., Sbrana, E., Peterson, J. W., Brasel, T., LeDuc, J. W., Endsley, J. J., & Torres, A. G. (2014). Comparative Burkholderia pseudomallei natural history virulence studies using an aerosol murine model of infection. Scientific Reports, 4, 4305. https://doi.org/10.1038/srep04305
Massey S, et al. Comparative Burkholderia Pseudomallei Natural History Virulence Studies Using an Aerosol Murine Model of Infection. Sci Rep. 2014 Mar 7;4:4305. PubMed PMID: 24603493.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative Burkholderia pseudomallei natural history virulence studies using an aerosol murine model of infection. AU - Massey,Shane, AU - Yeager,Linsey A, AU - Blumentritt,Carla A, AU - Vijayakumar,Sudhamathi, AU - Sbrana,Elena, AU - Peterson,Johnny W, AU - Brasel,Trevor, AU - LeDuc,James W, AU - Endsley,Janice J, AU - Torres,Alfredo G, Y1 - 2014/03/07/ PY - 2014/01/02/received PY - 2014/02/13/accepted PY - 2014/3/8/entrez PY - 2014/3/8/pubmed PY - 2015/10/21/medline SP - 4305 EP - 4305 JF - Scientific reports JO - Sci Rep VL - 4 N2 - Melioidosis is an endemic disease caused by the bacterium Burkholderia pseudomallei. Concerns exist regarding B. pseudomallei use as a potential bio-threat agent causing persistent infections and typically manifesting as severe pneumonia capable of causing fatal bacteremia. Development of suitable therapeutics against melioidosis is complicated due to high degree of genetic and phenotypic variability among B. pseudomallei isolates and lack of data establishing commonly accepted strains for comparative studies. Further, the impact of strain variation on virulence, disease presentation, and mortality is not well understood. Therefore, this study evaluate and compare the virulence and disease progression of B. pseudomallei strains K96243 and HBPUB10303a, following aerosol challenge in a standardized BALB/c mouse model of infection. The natural history analysis of disease progression monitored conditions such as weight, body temperature, appearance, activity, bacteremia, organ and tissue colonization (pathological and histological analysis) and immunological responses. This study provides a detailed, direct comparison of infection with different B. pseudomallei strains and set up the basis for a standardized model useful to test different medical countermeasures against Burkholderia species. Further, this protocol serves as a guideline to standardize other bacterial aerosol models of infection or to define biomarkers of infectious processes caused by other intracellular pathogens. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/24603493/Comparative_Burkholderia_pseudomallei_natural_history_virulence_studies_using_an_aerosol_murine_model_of_infection_ L2 - https://doi.org/10.1038/srep04305 DB - PRIME DP - Unbound Medicine ER -