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The venom of the spider Selenocosmia jiafu contains various neurotoxins acting on voltage-gated ion channels in rat dorsal root ganglion neurons.
Toxins (Basel). 2014 Mar 05; 6(3):988-1001.T

Abstract

Selenocosmia jiafu is a medium-sized theraphosid spider and an attractive source of venom, because it can be bred in captivity and it produces large amounts of venom. We performed reversed-phase high-performance liquid chromatography (RP-HPLC) and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analyses and showed that S. jiafu venom contains hundreds of peptides with a predominant mass of 3000-4500 Da. Patch clamp analyses indicated that the venom could inhibit voltage-gated Na+, K+ and Ca2+ channels in rat dorsal root ganglion (DRG) neurons. The venom exhibited inhibitory effects on tetrodotoxin-resistant (TTX-R) Na+ currents and T-type Ca2+ currents, suggesting the presence of antagonists to both channel types and providing a valuable tool for the investigation of these channels and for drug development. Intra-abdominal injection of the venom had severe toxic effects on cockroaches and caused death at higher concentrations. The LD50 was 84.24 μg/g of body weight in the cockroach. However, no visible symptoms or behavioral changes were detected after intraperitoneal injection of the venom into mice even at doses up to 10 mg/kg body weight. Our results provide a basis for further case-by-case investigations of peptide toxins from this venom.

Authors+Show Affiliations

Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China. 405542716@qq.com.Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China. zhouxi016@163.com.Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China. chenjia113322@163.com.Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China. tangcheng1985ss@sina.com.Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Department of Life Science, Huaihua College, Huaihua, Hunan 418008, China. 405542716@qq.com.Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China. dazhongyin002@126.com.Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China. liuzh@hunnu.edu.cn.Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China. liangsp@hunnu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24603666

Citation

Hu, Zhaotun, et al. "The Venom of the Spider Selenocosmia Jiafu Contains Various Neurotoxins Acting On Voltage-gated Ion Channels in Rat Dorsal Root Ganglion Neurons." Toxins, vol. 6, no. 3, 2014, pp. 988-1001.
Hu Z, Zhou X, Chen J, et al. The venom of the spider Selenocosmia jiafu contains various neurotoxins acting on voltage-gated ion channels in rat dorsal root ganglion neurons. Toxins (Basel). 2014;6(3):988-1001.
Hu, Z., Zhou, X., Chen, J., Tang, C., Xiao, Z., Ying, D., Liu, Z., & Liang, S. (2014). The venom of the spider Selenocosmia jiafu contains various neurotoxins acting on voltage-gated ion channels in rat dorsal root ganglion neurons. Toxins, 6(3), 988-1001. https://doi.org/10.3390/toxins6030988
Hu Z, et al. The Venom of the Spider Selenocosmia Jiafu Contains Various Neurotoxins Acting On Voltage-gated Ion Channels in Rat Dorsal Root Ganglion Neurons. Toxins (Basel). 2014 Mar 5;6(3):988-1001. PubMed PMID: 24603666.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The venom of the spider Selenocosmia jiafu contains various neurotoxins acting on voltage-gated ion channels in rat dorsal root ganglion neurons. AU - Hu,Zhaotun, AU - Zhou,Xi, AU - Chen,Jia, AU - Tang,Cheng, AU - Xiao,Zhen, AU - Ying,Dazhong, AU - Liu,Zhonghua, AU - Liang,Songping, Y1 - 2014/03/05/ PY - 2014/01/27/received PY - 2014/02/10/revised PY - 2014/02/17/accepted PY - 2014/3/8/entrez PY - 2014/3/8/pubmed PY - 2014/11/2/medline SP - 988 EP - 1001 JF - Toxins JO - Toxins (Basel) VL - 6 IS - 3 N2 - Selenocosmia jiafu is a medium-sized theraphosid spider and an attractive source of venom, because it can be bred in captivity and it produces large amounts of venom. We performed reversed-phase high-performance liquid chromatography (RP-HPLC) and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analyses and showed that S. jiafu venom contains hundreds of peptides with a predominant mass of 3000-4500 Da. Patch clamp analyses indicated that the venom could inhibit voltage-gated Na+, K+ and Ca2+ channels in rat dorsal root ganglion (DRG) neurons. The venom exhibited inhibitory effects on tetrodotoxin-resistant (TTX-R) Na+ currents and T-type Ca2+ currents, suggesting the presence of antagonists to both channel types and providing a valuable tool for the investigation of these channels and for drug development. Intra-abdominal injection of the venom had severe toxic effects on cockroaches and caused death at higher concentrations. The LD50 was 84.24 μg/g of body weight in the cockroach. However, no visible symptoms or behavioral changes were detected after intraperitoneal injection of the venom into mice even at doses up to 10 mg/kg body weight. Our results provide a basis for further case-by-case investigations of peptide toxins from this venom. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/24603666/The_venom_of_the_spider_Selenocosmia_jiafu_contains_various_neurotoxins_acting_on_voltage_gated_ion_channels_in_rat_dorsal_root_ganglion_neurons_ L2 - http://www.mdpi.com/resolver?pii=toxins6030988 DB - PRIME DP - Unbound Medicine ER -