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Encephalitogenic T cell clones with variant receptor specificity.
J Immunol. 1988 Dec 01; 141(11):3828-32.JI

Abstract

We explored antigenic differences between guinea pig (GP)-basic protein (BP), rat (Rt)-BP, and respective peptides from the encephalitogenic region for Lewis rats by comparing the fine specificity of T lymphocyte lines and clones selected from animals primed with these Ag. Encephalitogenic T cell lines specific for GP-BP or Rt-BP predictably recognized the corresponding 72-89 and to a lesser degree the 72-84 (S55S) amino acid sequence. T cell lines selected from rats primed with GP-S55S responded preferentially to GP-S55S compared to other peptides. A T cell line raised to Rt-S55S, however, initially recognized the S55S and S72-89 peptides but were nearly unresponsive to the intact GP-BP or Rt-BP. T cell clones selected from the Rt-S55S line at that point had two distinct patterns of response: clones that recognized both of the BP and the S55S peptides adoptively transferred delayed-type hypersensitivity and experimental autoimmune encephalomyelitis. These clones also recognized residues 69-81 (S67) but not peptide S75-89. In contrast, T cell clones that responded only to synthetic peptides GP-S55S and Rt-S55S but not to the parent BP adoptively transferred delayed-type hypersensitivity but not disease in Lewis rats. The same clones failed to respond to either the S67 or the S75-89 sequences. These results demonstrate that the encephalitogenic Rt-S55S sequence houses a minimum of two T cell epitopes with differing specificities and functions. One epitope is immuno-dominant and resembles the encephalitogenic region of the intact BP molecule. The second non-encephalitogenic epitope is restricted to the S55S sequences and is not shared by the parent BP, the S67, or the S75-89 sequences. Both types of Rt-S55S-specific clones differ in fine specificity from encephalitogenic clones selected from GP-BP immunized rats, thus indicating that uniformity of T cell recognition of the encephalitogenic epitope is not an absolute condition for T cells to be encephalitogenic.

Authors+Show Affiliations

Neuroimmunology Research, V.A. Medical Center, Portland, OR 97201.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2460551

Citation

Offner, H, et al. "Encephalitogenic T Cell Clones With Variant Receptor Specificity." Journal of Immunology (Baltimore, Md. : 1950), vol. 141, no. 11, 1988, pp. 3828-32.
Offner H, Hashim GA, Chou YK, et al. Encephalitogenic T cell clones with variant receptor specificity. J Immunol. 1988;141(11):3828-32.
Offner, H., Hashim, G. A., Chou, Y. K., Celnik, B., Jones, R., & Vandenbark, A. A. (1988). Encephalitogenic T cell clones with variant receptor specificity. Journal of Immunology (Baltimore, Md. : 1950), 141(11), 3828-32.
Offner H, et al. Encephalitogenic T Cell Clones With Variant Receptor Specificity. J Immunol. 1988 Dec 1;141(11):3828-32. PubMed PMID: 2460551.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Encephalitogenic T cell clones with variant receptor specificity. AU - Offner,H, AU - Hashim,G A, AU - Chou,Y K, AU - Celnik,B, AU - Jones,R, AU - Vandenbark,A A, PY - 1988/12/1/pubmed PY - 1988/12/1/medline PY - 1988/12/1/entrez SP - 3828 EP - 32 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 141 IS - 11 N2 - We explored antigenic differences between guinea pig (GP)-basic protein (BP), rat (Rt)-BP, and respective peptides from the encephalitogenic region for Lewis rats by comparing the fine specificity of T lymphocyte lines and clones selected from animals primed with these Ag. Encephalitogenic T cell lines specific for GP-BP or Rt-BP predictably recognized the corresponding 72-89 and to a lesser degree the 72-84 (S55S) amino acid sequence. T cell lines selected from rats primed with GP-S55S responded preferentially to GP-S55S compared to other peptides. A T cell line raised to Rt-S55S, however, initially recognized the S55S and S72-89 peptides but were nearly unresponsive to the intact GP-BP or Rt-BP. T cell clones selected from the Rt-S55S line at that point had two distinct patterns of response: clones that recognized both of the BP and the S55S peptides adoptively transferred delayed-type hypersensitivity and experimental autoimmune encephalomyelitis. These clones also recognized residues 69-81 (S67) but not peptide S75-89. In contrast, T cell clones that responded only to synthetic peptides GP-S55S and Rt-S55S but not to the parent BP adoptively transferred delayed-type hypersensitivity but not disease in Lewis rats. The same clones failed to respond to either the S67 or the S75-89 sequences. These results demonstrate that the encephalitogenic Rt-S55S sequence houses a minimum of two T cell epitopes with differing specificities and functions. One epitope is immuno-dominant and resembles the encephalitogenic region of the intact BP molecule. The second non-encephalitogenic epitope is restricted to the S55S sequences and is not shared by the parent BP, the S67, or the S75-89 sequences. Both types of Rt-S55S-specific clones differ in fine specificity from encephalitogenic clones selected from GP-BP immunized rats, thus indicating that uniformity of T cell recognition of the encephalitogenic epitope is not an absolute condition for T cells to be encephalitogenic. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2460551/Encephalitogenic_T_cell_clones_with_variant_receptor_specificity_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2460551 DB - PRIME DP - Unbound Medicine ER -