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Bactericidal application and cytotoxic activity of biosynthesized silver nanoparticles with an extract of the red seaweed Pterocladiella capillacea on the HepG2 cell line.
Asian Pac J Cancer Prev. 2014; 15(3):1299-306.AP

Abstract

BACKGROUND

Nano-biotechnology is recognized as offering revolutionary changes in various fields of medicine. Biologically synthesized silver nanoparticles have a wide range of applications.

MATERIALS AND METHODS

Silver nanoparticles (AgNPs) were biosynthesized with an aqueous extract of Pterocladiella (Pterocladia) capillacea, used as a reducing and stabilizing agent, and characterized using UV-VIS spectroscopy, Fourier Transform Infra red (FT-IR) spectroscopy, transmission electron microscopy (TEM) and energy dispersive analysis (EDX). The biosynthesized AgNPs were tested for cytotoxic activity in a human hepatocellular carcinoma (HepG2) cell line cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum, 1% antibiotic and antimycotic solution and 2 mM glutamine. Bacterial susceptibility to AgNPs was assessed with Staphylococcus aureus, Bacillus subtilis [Gram+ve] and Pseudomonas aeruginosa and Escherichia coli [Gram-ve]. The agar well diffusion technique was adopted to evaluate the bactericidal activity of the biosynthesized AgNPs using Ampicillin and Gentamicin as gram+ve and gram-ve antibacterial standard drugs, respectively.

RESULTS

The biosynthesized AgNPs were 11.4±3.52 nm in diameter. FT-IR analysis showed that carbonyl groups from the amino acid residues and proteins could assist in formation and stabilization of AgNPs. The AgNPs showed potent cytotoxic activity against the human hepatocellular carcinoma (HepG2) cell line at higher concentrations. The results also showed that the biosynthesized AgNPs inhibited the entire panel of tested bacteria with a marked specificity towards Bacillus subtillus.

CONCLUSIONS

Cytotoxic activity of the biosynthesized AgNPs may be due to the presence of alkaloids present in the algal extract. Our AgNPs appear more bactericidal against gram-positive bacteria (B. subtillus).

Authors+Show Affiliations

El Kassas HY
Department of Hydrobiology, National Institute of Oceanography and Fisheries, Alexandria, Egypt E-mail : halayassin12@yahoo.com.
Attia AA
No affiliation info available

MeSH

AmpicillinAnti-Bacterial AgentsAntineoplastic AgentsBacillus subtilisCarcinoma, HepatocellularCell Line, TumorEscherichia coliGentamicinsHep G2 CellsHumansLiver NeoplasmsMetal NanoparticlesMicrobial Sensitivity TestsPlant ExtractsPseudomonas aeruginosaRhodophytaSilverSilver NitrateStaphylococcus aureus

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24606456

Citation

El Kassas, Hala Yassin, and Azza Ahmed Attia. "Bactericidal Application and Cytotoxic Activity of Biosynthesized Silver Nanoparticles With an Extract of the Red Seaweed Pterocladiella Capillacea On the HepG2 Cell Line." Asian Pacific Journal of Cancer Prevention : APJCP, vol. 15, no. 3, 2014, pp. 1299-306.
El Kassas HY, Attia AA. Bactericidal application and cytotoxic activity of biosynthesized silver nanoparticles with an extract of the red seaweed Pterocladiella capillacea on the HepG2 cell line. Asian Pac J Cancer Prev. 2014;15(3):1299-306.
El Kassas, H. Y., & Attia, A. A. (2014). Bactericidal application and cytotoxic activity of biosynthesized silver nanoparticles with an extract of the red seaweed Pterocladiella capillacea on the HepG2 cell line. Asian Pacific Journal of Cancer Prevention : APJCP, 15(3), 1299-306.
El Kassas HY, Attia AA. Bactericidal Application and Cytotoxic Activity of Biosynthesized Silver Nanoparticles With an Extract of the Red Seaweed Pterocladiella Capillacea On the HepG2 Cell Line. Asian Pac J Cancer Prev. 2014;15(3):1299-306. PubMed PMID: 24606456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bactericidal application and cytotoxic activity of biosynthesized silver nanoparticles with an extract of the red seaweed Pterocladiella capillacea on the HepG2 cell line. AU - El Kassas,Hala Yassin, AU - Attia,Azza Ahmed, PY - 2014/3/11/entrez PY - 2014/3/13/pubmed PY - 2014/11/19/medline SP - 1299 EP - 306 JF - Asian Pacific journal of cancer prevention : APJCP JO - Asian Pac J Cancer Prev VL - 15 IS - 3 N2 - BACKGROUND: Nano-biotechnology is recognized as offering revolutionary changes in various fields of medicine. Biologically synthesized silver nanoparticles have a wide range of applications. MATERIALS AND METHODS: Silver nanoparticles (AgNPs) were biosynthesized with an aqueous extract of Pterocladiella (Pterocladia) capillacea, used as a reducing and stabilizing agent, and characterized using UV-VIS spectroscopy, Fourier Transform Infra red (FT-IR) spectroscopy, transmission electron microscopy (TEM) and energy dispersive analysis (EDX). The biosynthesized AgNPs were tested for cytotoxic activity in a human hepatocellular carcinoma (HepG2) cell line cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum, 1% antibiotic and antimycotic solution and 2 mM glutamine. Bacterial susceptibility to AgNPs was assessed with Staphylococcus aureus, Bacillus subtilis [Gram+ve] and Pseudomonas aeruginosa and Escherichia coli [Gram-ve]. The agar well diffusion technique was adopted to evaluate the bactericidal activity of the biosynthesized AgNPs using Ampicillin and Gentamicin as gram+ve and gram-ve antibacterial standard drugs, respectively. RESULTS: The biosynthesized AgNPs were 11.4±3.52 nm in diameter. FT-IR analysis showed that carbonyl groups from the amino acid residues and proteins could assist in formation and stabilization of AgNPs. The AgNPs showed potent cytotoxic activity against the human hepatocellular carcinoma (HepG2) cell line at higher concentrations. The results also showed that the biosynthesized AgNPs inhibited the entire panel of tested bacteria with a marked specificity towards Bacillus subtillus. CONCLUSIONS: Cytotoxic activity of the biosynthesized AgNPs may be due to the presence of alkaloids present in the algal extract. Our AgNPs appear more bactericidal against gram-positive bacteria (B. subtillus). SN - 2476-762X UR - https://www.unboundmedicine.com/medline/citation/24606456/Bactericidal_application_and_cytotoxic_activity_of_biosynthesized_silver_nanoparticles_with_an_extract_of_the_red_seaweed_Pterocladiella_capillacea_on_the_HepG2_cell_line_ DB - PRIME DP - Unbound Medicine ER -