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YAP expression in normal and neoplastic breast tissue: an immunohistochemical study.
Arch Med Res. 2014 Apr; 45(3):223-8.AM

Abstract

BACKGROUND AND AIMS

Yes-associated protein (YAP) is a transcriptional factor involved in normal cell proliferation, apoptosis and carcinogenesis; however, its contribution to breast cancer (BC) is still controversial. We undertook this study to compare the expression of YAP by immunohistochemistry (IHC) in normal breast tissue of women without breast cancer (BC) (controls), non-neoplastic breast tissue in women with cancer (internal controls) and in four different subtypes of invasive ductal carcinoma.

METHODS

There were 17 controls and 105 tumor cases (53 luminal A, 15 luminal B, 20 overexpression of HER2 and 17 triple negative cases) studied by IHC. Statistical analysis included χ(2) for linear trend (Extended Mantel-Haenszel).

RESULTS

There were 40% of internal controls that showed expression of YAP in myoepithelial cells, whereas in controls expression was 100%. In controls, 3/17 (17.6%) showed cytoplasmic staining in luminal cells. There was a significant difference in nuclear expression between the ductal BC subtypes. Luminal A had 4% of positive cases with <10% of cells affected in each case; in contrast, there were 17-20% of positive cases in the other groups with 50% or more of stained cells. YAP expression in stromal cells was not observed in controls or in triple-negative cases, and luminal B pattern had the highest YAP nuclear expression (20%).

CONCLUSIONS

YAP showed decreased expression in tumor cells compared with normal breast tissue. These findings are consistent with a role of YAP as a suppressor gene in BC and show differences in YAP expression in different patterns of ductal BC.

Authors+Show Affiliations

Instituto de Medicina Forense, Juan Pablo II, Esq, Reyes Heroles Fracc. Costa Verde, Boca del Río, Veracruz, Mexico.Facultad de Enfermería, Universidad Autónoma de Nuevo León, Monterrey, NL, Mexico.Instituto de Medicina Forense, Universidad Veracruzana, Boca del Río, Veracruz, Mexico.Centro de Investigación Biomédica de la Facultad de Medicina, Universidad Autónoma de Coahuila, Torréon, Coahuila, Mexico.Instituto de Medicina Forense, Universidad Veracruzana, Boca del Río, Veracruz, Mexico. Electronic address: acald911@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24606817

Citation

Jaramillo-Rodríguez, Yolanda, et al. "YAP Expression in Normal and Neoplastic Breast Tissue: an Immunohistochemical Study." Archives of Medical Research, vol. 45, no. 3, 2014, pp. 223-8.
Jaramillo-Rodríguez Y, Cerda-Flores RM, Ruiz-Ramos R, et al. YAP expression in normal and neoplastic breast tissue: an immunohistochemical study. Arch Med Res. 2014;45(3):223-8.
Jaramillo-Rodríguez, Y., Cerda-Flores, R. M., Ruiz-Ramos, R., López-Márquez, F. C., & Calderón-Garcidueñas, A. L. (2014). YAP expression in normal and neoplastic breast tissue: an immunohistochemical study. Archives of Medical Research, 45(3), 223-8. https://doi.org/10.1016/j.arcmed.2014.01.010
Jaramillo-Rodríguez Y, et al. YAP Expression in Normal and Neoplastic Breast Tissue: an Immunohistochemical Study. Arch Med Res. 2014;45(3):223-8. PubMed PMID: 24606817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - YAP expression in normal and neoplastic breast tissue: an immunohistochemical study. AU - Jaramillo-Rodríguez,Yolanda, AU - Cerda-Flores,Ricardo M, AU - Ruiz-Ramos,Ruben, AU - López-Márquez,Francisco C, AU - Calderón-Garcidueñas,Ana Laura, Y1 - 2014/03/04/ PY - 2013/09/25/received PY - 2014/01/29/accepted PY - 2014/3/11/entrez PY - 2014/3/13/pubmed PY - 2014/6/26/medline KW - Breast cancer KW - Immunohistochemistry KW - Yes-associated protein SP - 223 EP - 8 JF - Archives of medical research JO - Arch. Med. Res. VL - 45 IS - 3 N2 - BACKGROUND AND AIMS: Yes-associated protein (YAP) is a transcriptional factor involved in normal cell proliferation, apoptosis and carcinogenesis; however, its contribution to breast cancer (BC) is still controversial. We undertook this study to compare the expression of YAP by immunohistochemistry (IHC) in normal breast tissue of women without breast cancer (BC) (controls), non-neoplastic breast tissue in women with cancer (internal controls) and in four different subtypes of invasive ductal carcinoma. METHODS: There were 17 controls and 105 tumor cases (53 luminal A, 15 luminal B, 20 overexpression of HER2 and 17 triple negative cases) studied by IHC. Statistical analysis included χ(2) for linear trend (Extended Mantel-Haenszel). RESULTS: There were 40% of internal controls that showed expression of YAP in myoepithelial cells, whereas in controls expression was 100%. In controls, 3/17 (17.6%) showed cytoplasmic staining in luminal cells. There was a significant difference in nuclear expression between the ductal BC subtypes. Luminal A had 4% of positive cases with <10% of cells affected in each case; in contrast, there were 17-20% of positive cases in the other groups with 50% or more of stained cells. YAP expression in stromal cells was not observed in controls or in triple-negative cases, and luminal B pattern had the highest YAP nuclear expression (20%). CONCLUSIONS: YAP showed decreased expression in tumor cells compared with normal breast tissue. These findings are consistent with a role of YAP as a suppressor gene in BC and show differences in YAP expression in different patterns of ductal BC. SN - 1873-5487 UR - https://www.unboundmedicine.com/medline/citation/24606817/YAP_expression_in_normal_and_neoplastic_breast_tissue:_an_immunohistochemical_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0188-4409(14)00011-3 DB - PRIME DP - Unbound Medicine ER -