Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of human insulin in plasma and pharmaceutical formulations.
Talanta. 2014 Apr; 121:30-6.T

Abstract

In this paper, a novel method is described for automated determination of human insulin in biological fluids using principle of sequential injection on a molecularly imprinted solid-phase extraction (MISPE) cartridge as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, chloroform as a porogen and insulin as a template molecule. The imprinted polymers were then employed as the solid-phase extraction sorbent for on-line extraction of insulin from human plasma samples. To achieve the best condition, influential parameters on the extraction efficiency were thoroughly investigated. Rapid and simple analysis of the hormone was successfully accomplished through the good selectivity of the prepared sorbent coupled with HPLC. Limits of detection (LOD) and quantification (LOQ) of 0.2 ng mL(-1), 0.7 ng mL(-1), and 0.03 ng mL(-1), 0.1 ng mL(-1) were obtained in plasma and urine respectively. The obtained data exhibited the great recoveries for extraction of insulin from human plasma and pharmaceutical samples, higher than 87%.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Moein MM

Department of Chemistry, Amirkabir University of Technology, Tehran, Iran.

Javanbakht M

Department of Chemistry, Amirkabir University of Technology, Tehran, Iran. Electronic address: mehranjavanbakht@gmail.com.

Akbari-adergani B

Food & Drug Laboratory Research Center, Food & Drug Organization, Ministry of Health and Medical Education, Tehran, Iran.

MeSH

AdsorptionCalibrationChromatography, High Pressure LiquidHumansHydrogen-Ion ConcentrationInsulinLimit of DetectionMicroscopy, Electron, ScanningMolecular ImprintingPharmaceutical PreparationsReproducibility of ResultsSolid Phase Extraction

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24607106

Citation

Moein, Mohammad Mahdi, et al. "Molecularly Imprinted Polymer Cartridges Coupled On-line With High Performance Liquid Chromatography for Simple and Rapid Analysis of Human Insulin in Plasma and Pharmaceutical Formulations." Talanta, vol. 121, 2014, pp. 30-6.

Moein MM, Javanbakht M, Akbari-adergani B. Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of human insulin in plasma and pharmaceutical formulations. Talanta. 2014;121:30-6.

Moein, M. M., Javanbakht, M., & Akbari-adergani, B. (2014). Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of human insulin in plasma and pharmaceutical formulations. Talanta, 121, 30-6. https://doi.org/10.1016/j.talanta.2013.12.011

Moein MM, Javanbakht M, Akbari-adergani B. Molecularly Imprinted Polymer Cartridges Coupled On-line With High Performance Liquid Chromatography for Simple and Rapid Analysis of Human Insulin in Plasma and Pharmaceutical Formulations. Talanta. 2014;121:30-6. PubMed PMID: 24607106.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of human insulin in plasma and pharmaceutical formulations. AU - Moein,Mohammad Mahdi, AU - Javanbakht,Mehran, AU - Akbari-adergani,Behrouz, Y1 - 2013/12/31/ PY - 2013/08/06/received PY - 2013/12/02/revised PY - 2013/12/04/accepted PY - 2014/3/11/entrez PY - 2014/3/13/pubmed PY - 2014/10/28/medline KW - Automated KW - Human plasma KW - Insulin KW - Molecularly imprinted polymer KW - Pharmaceutical formulations KW - Sample clean-up SP - 30 EP - 6 JF - Talanta JO - Talanta VL - 121 N2 - In this paper, a novel method is described for automated determination of human insulin in biological fluids using principle of sequential injection on a molecularly imprinted solid-phase extraction (MISPE) cartridge as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, chloroform as a porogen and insulin as a template molecule. The imprinted polymers were then employed as the solid-phase extraction sorbent for on-line extraction of insulin from human plasma samples. To achieve the best condition, influential parameters on the extraction efficiency were thoroughly investigated. Rapid and simple analysis of the hormone was successfully accomplished through the good selectivity of the prepared sorbent coupled with HPLC. Limits of detection (LOD) and quantification (LOQ) of 0.2 ng mL(-1), 0.7 ng mL(-1), and 0.03 ng mL(-1), 0.1 ng mL(-1) were obtained in plasma and urine respectively. The obtained data exhibited the great recoveries for extraction of insulin from human plasma and pharmaceutical samples, higher than 87%. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/24607106/Molecularly_imprinted_polymer_cartridges_coupled_on_line_with_high_performance_liquid_chromatography_for_simple_and_rapid_analysis_of_human_insulin_in_plasma_and_pharmaceutical_formulations_ DB - PRIME DP - Unbound Medicine ER -

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Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of human insulin in plasma and pharmaceutical formulations.Talanta. 2014 Apr; 121:30-6.T