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Electrochemiluminescence aptasensor for adenosine triphosphate detection using host-guest recognition between metallocyclodextrin complex and aptamer.
Talanta. 2014 Apr; 121:229-33.T

Abstract

A sensitive and label-free electrochemiluminescence (ECL) aptasensor for the detection of adenosine triphosphate (ATP) was successfully designed using host-guest recognition between a metallocyclodextrin complex, i.e., tris(bipyridine)ruthenium(II)-β-cyclodextrin [tris(bpyRu)-β-CD], and an ATP-binding aptamer. In the protocol, the NH2-terminated aptamer was immobilized on a glassy carbon electrode (GCE) by a coupling interaction. After host-guest recognition between tris(bpyRu)-β-CD and aptamer, the tris(bpyRu)-β-CD/aptamer/GCE produced a strong ECL signal as a result of the photoactive properties of tris(bpyRu)-β-CD. However, in the presence of ATP, the ATP/aptamer complex was formed preferentially, which restricted host-guest recognition, and therefore less tris(bpyRu)-β-CD was attached to the GCE surface, resulting in an obvious decrease in the ECL intensity. Under optimal determination conditions, an excellent logarithmic linear relationship between the ECL decrease and ATP concentration was obtained in the range 10.0-0.05 nM, with a detection limit of 0.01 nM at the S/N ratio of 3. The proposed ECL-based ATP aptasensor exhibited high sensitivity and selectivity, without time-consuming signal-labeling procedures, and is considered to be a promising model for detection of aptamer-specific targets.

Authors+Show Affiliations

Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China. Electronic address: pghe@chem.ecnu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24607132

Citation

Chen, Hong, et al. "Electrochemiluminescence Aptasensor for Adenosine Triphosphate Detection Using Host-guest Recognition Between Metallocyclodextrin Complex and Aptamer." Talanta, vol. 121, 2014, pp. 229-33.
Chen H, Chen Q, Zhao Y, et al. Electrochemiluminescence aptasensor for adenosine triphosphate detection using host-guest recognition between metallocyclodextrin complex and aptamer. Talanta. 2014;121:229-33.
Chen, H., Chen, Q., Zhao, Y., Zhang, F., Yang, F., Tang, J., & He, P. (2014). Electrochemiluminescence aptasensor for adenosine triphosphate detection using host-guest recognition between metallocyclodextrin complex and aptamer. Talanta, 121, 229-33. https://doi.org/10.1016/j.talanta.2013.12.039
Chen H, et al. Electrochemiluminescence Aptasensor for Adenosine Triphosphate Detection Using Host-guest Recognition Between Metallocyclodextrin Complex and Aptamer. Talanta. 2014;121:229-33. PubMed PMID: 24607132.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemiluminescence aptasensor for adenosine triphosphate detection using host-guest recognition between metallocyclodextrin complex and aptamer. AU - Chen,Hong, AU - Chen,Qiong, AU - Zhao,Yingying, AU - Zhang,Fan, AU - Yang,Fan, AU - Tang,Jie, AU - He,Pingang, Y1 - 2014/01/07/ PY - 2013/10/27/received PY - 2013/12/18/revised PY - 2013/12/20/accepted PY - 2014/3/11/entrez PY - 2014/3/13/pubmed PY - 2014/10/28/medline KW - Adenosine triphosphate KW - Aptasensor KW - Electrochemiluminescence KW - Host–guest recognition KW - Metallocyclodextrin SP - 229 EP - 33 JF - Talanta JO - Talanta VL - 121 N2 - A sensitive and label-free electrochemiluminescence (ECL) aptasensor for the detection of adenosine triphosphate (ATP) was successfully designed using host-guest recognition between a metallocyclodextrin complex, i.e., tris(bipyridine)ruthenium(II)-β-cyclodextrin [tris(bpyRu)-β-CD], and an ATP-binding aptamer. In the protocol, the NH2-terminated aptamer was immobilized on a glassy carbon electrode (GCE) by a coupling interaction. After host-guest recognition between tris(bpyRu)-β-CD and aptamer, the tris(bpyRu)-β-CD/aptamer/GCE produced a strong ECL signal as a result of the photoactive properties of tris(bpyRu)-β-CD. However, in the presence of ATP, the ATP/aptamer complex was formed preferentially, which restricted host-guest recognition, and therefore less tris(bpyRu)-β-CD was attached to the GCE surface, resulting in an obvious decrease in the ECL intensity. Under optimal determination conditions, an excellent logarithmic linear relationship between the ECL decrease and ATP concentration was obtained in the range 10.0-0.05 nM, with a detection limit of 0.01 nM at the S/N ratio of 3. The proposed ECL-based ATP aptasensor exhibited high sensitivity and selectivity, without time-consuming signal-labeling procedures, and is considered to be a promising model for detection of aptamer-specific targets. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/24607132/Electrochemiluminescence_aptasensor_for_adenosine_triphosphate_detection_using_host_guest_recognition_between_metallocyclodextrin_complex_and_aptamer_ DB - PRIME DP - Unbound Medicine ER -