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The contribution of transient receptor potential ankyrin 1 (TRPA1) to the in vivo nociceptive effects of prostaglandin E₂.
Life Sci. 2014 Jun 06; 105(1-2):7-13.LS

Abstract

AIMS

Although evidence suggest that TRPA1 mediates some effects of prostaglandins, it is not known whether TRPA1 contributes to the in vivo nociceptive effects of prostaglandin E2 (PGE2), a key mediator of inflammatory pain.

MAIN METHODS

To address this issue, the effect of the pharmacological blockade of TRPA1 or of its gene silencing on the hyperalgesia induced in the rat paw by PGE2 or its downstream signaling molecules, protein kinase A (PKA) or protein kinase C-epsilon (PKCε), was evaluated. TRPA1 expression on dorsal root ganglia cells was assessed by western blot.

KEY FINDINGS

The pharmacological blockade of local TRPA1 by its selective antagonist, HC 030031 decreased and reversed PGE2-induced hyperalgesia. The TRPA1 gene silencing induced by intrathecal pre-treatment with antisense oligodeoxynucleotide blocked PGE2-induced hyperalgesia and strongly reduced TRPA1 expression in dorsal root ganglia cells (L5 and L6). PGE2 injection into the hind paw did not significantly increase TRPA1 expression in dorsal root ganglia cells. Treatment with either HC 030031 or antisense oligodeoxynucleotide significantly decreased the hyperalgesia induced by PKA or PKCε. Since both kinases are the major components of PGE2-induced intracellular signal transduction, the modulation of TRPA1 function by PGE2 may be downstream PKA and PKC-epsilon.

SIGNIFICANCE

These findings show that TRPA1 is essential to the in vivo nociceptive effects induced by one of the most important mediators of inflammatory pain, PGE2. This is one of the crucial findings necessary to support TRPA1 as a promising target for the development of future drugs to pain treatment and control.

Authors+Show Affiliations

Department of Physiology, Division of Biological Sciences, Federal University of Parana, Curitiba, Parana, Brazil.Department of Structural and Functional Biology, Institute of Biology, State University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.Department of Pharmacology, Division of Biological Sciences, Federal University of Parana, Curitiba, Parana, Brazil.Department of Structural and Functional Biology, Institute of Biology, State University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.Department of Structural and Functional Biology, Institute of Biology, State University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.Department of Physiology, Division of Biological Sciences, Federal University of Parana, Curitiba, Parana, Brazil. Electronic address: fischer@ufpr.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24607781

Citation

Dall'Acqua, Marcelo C., et al. "The Contribution of Transient Receptor Potential Ankyrin 1 (TRPA1) to the in Vivo Nociceptive Effects of Prostaglandin E₂." Life Sciences, vol. 105, no. 1-2, 2014, pp. 7-13.
Dall'Acqua MC, Bonet IJ, Zampronio AR, et al. The contribution of transient receptor potential ankyrin 1 (TRPA1) to the in vivo nociceptive effects of prostaglandin E₂. Life Sci. 2014;105(1-2):7-13.
Dall'Acqua, M. C., Bonet, I. J., Zampronio, A. R., Tambeli, C. H., Parada, C. A., & Fischer, L. (2014). The contribution of transient receptor potential ankyrin 1 (TRPA1) to the in vivo nociceptive effects of prostaglandin E₂. Life Sciences, 105(1-2), 7-13. https://doi.org/10.1016/j.lfs.2014.02.031
Dall'Acqua MC, et al. The Contribution of Transient Receptor Potential Ankyrin 1 (TRPA1) to the in Vivo Nociceptive Effects of Prostaglandin E₂. Life Sci. 2014 Jun 6;105(1-2):7-13. PubMed PMID: 24607781.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The contribution of transient receptor potential ankyrin 1 (TRPA1) to the in vivo nociceptive effects of prostaglandin E₂. AU - Dall'Acqua,Marcelo C, AU - Bonet,Ivan J M, AU - Zampronio,Aleksander R, AU - Tambeli,Cláudia H, AU - Parada,Carlos A, AU - Fischer,Luana, Y1 - 2014/03/06/ PY - 2013/10/30/received PY - 2014/01/28/revised PY - 2014/02/21/accepted PY - 2014/3/11/entrez PY - 2014/3/13/pubmed PY - 2014/7/16/medline KW - Inflammatory pain KW - Nociceptor sensitization KW - Prostaglandin E(2) KW - Protein kinase A KW - Protein kinase C-epsilon KW - TRPA1 SP - 7 EP - 13 JF - Life sciences JO - Life Sci VL - 105 IS - 1-2 N2 - AIMS: Although evidence suggest that TRPA1 mediates some effects of prostaglandins, it is not known whether TRPA1 contributes to the in vivo nociceptive effects of prostaglandin E2 (PGE2), a key mediator of inflammatory pain. MAIN METHODS: To address this issue, the effect of the pharmacological blockade of TRPA1 or of its gene silencing on the hyperalgesia induced in the rat paw by PGE2 or its downstream signaling molecules, protein kinase A (PKA) or protein kinase C-epsilon (PKCε), was evaluated. TRPA1 expression on dorsal root ganglia cells was assessed by western blot. KEY FINDINGS: The pharmacological blockade of local TRPA1 by its selective antagonist, HC 030031 decreased and reversed PGE2-induced hyperalgesia. The TRPA1 gene silencing induced by intrathecal pre-treatment with antisense oligodeoxynucleotide blocked PGE2-induced hyperalgesia and strongly reduced TRPA1 expression in dorsal root ganglia cells (L5 and L6). PGE2 injection into the hind paw did not significantly increase TRPA1 expression in dorsal root ganglia cells. Treatment with either HC 030031 or antisense oligodeoxynucleotide significantly decreased the hyperalgesia induced by PKA or PKCε. Since both kinases are the major components of PGE2-induced intracellular signal transduction, the modulation of TRPA1 function by PGE2 may be downstream PKA and PKC-epsilon. SIGNIFICANCE: These findings show that TRPA1 is essential to the in vivo nociceptive effects induced by one of the most important mediators of inflammatory pain, PGE2. This is one of the crucial findings necessary to support TRPA1 as a promising target for the development of future drugs to pain treatment and control. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/24607781/The_contribution_of_transient_receptor_potential_ankyrin_1__TRPA1__to_the_in_vivo_nociceptive_effects_of_prostaglandin_E₂_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(14)00288-4 DB - PRIME DP - Unbound Medicine ER -