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Interactions of manufactured silver nanoparticles of different sizes with normal human dermal fibroblasts.
Int Wound J. 2016 Feb; 13(1):101-9.IW

Abstract

Silver compounds have been used for their medicinal properties for centuries. At present, silver nanoparticles (AgNPs) are reemerging as a viable topical treatment option for infections encountered in burns, open wounds and chronic ulcers. This study evaluated the in vitro mechanisms of two different sizes of AgNPs (4·7 and 42 nm) toxicity in normal human dermal fibroblasts. The toxicity was evaluated by observing cell viability and oxidative stress parameters. In all toxicity endpoints studied (MTT and lactate dehydrogenase assays), AgNPs of 4·7 nm were much more toxic than the large AgNPs (42 nm). The cytotoxicity of both AgNPs was greatly decreased by pre-treatment with the antioxidant N-acetyl-L-cysteine. The oxidative stress parameters showed significant increase in reactive oxygen species levels, depletion of glutathione level and slight, but not statistically significant inactivation of superoxide dismutase, suggesting generation of oxidative stress. Thus, AgNPs should be used with caution for the topical treatment of burns and wounds, medical devices etc, because their toxicity depends on the size, the smaller NPs being much more cytotoxic than the large.

Authors+Show Affiliations

Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24612846

Citation

Avalos, Alicia, et al. "Interactions of Manufactured Silver Nanoparticles of Different Sizes With Normal Human Dermal Fibroblasts." International Wound Journal, vol. 13, no. 1, 2016, pp. 101-9.
Avalos A, Haza AI, Mateo D, et al. Interactions of manufactured silver nanoparticles of different sizes with normal human dermal fibroblasts. Int Wound J. 2016;13(1):101-9.
Avalos, A., Haza, A. I., Mateo, D., & Morales, P. (2016). Interactions of manufactured silver nanoparticles of different sizes with normal human dermal fibroblasts. International Wound Journal, 13(1), 101-9. https://doi.org/10.1111/iwj.12244
Avalos A, et al. Interactions of Manufactured Silver Nanoparticles of Different Sizes With Normal Human Dermal Fibroblasts. Int Wound J. 2016;13(1):101-9. PubMed PMID: 24612846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interactions of manufactured silver nanoparticles of different sizes with normal human dermal fibroblasts. AU - Avalos,Alicia, AU - Haza,Ana I, AU - Mateo,Diego, AU - Morales,Paloma, Y1 - 2014/02/25/ PY - 2013/10/31/received PY - 2014/01/23/revised PY - 2014/01/27/accepted PY - 2014/3/12/entrez PY - 2014/3/13/pubmed PY - 2016/11/12/medline KW - Cytotoxicity KW - Normal human dermal fibroblasts KW - Reactive oxygen species KW - Silver nanoparticles SP - 101 EP - 9 JF - International wound journal JO - Int Wound J VL - 13 IS - 1 N2 - Silver compounds have been used for their medicinal properties for centuries. At present, silver nanoparticles (AgNPs) are reemerging as a viable topical treatment option for infections encountered in burns, open wounds and chronic ulcers. This study evaluated the in vitro mechanisms of two different sizes of AgNPs (4·7 and 42 nm) toxicity in normal human dermal fibroblasts. The toxicity was evaluated by observing cell viability and oxidative stress parameters. In all toxicity endpoints studied (MTT and lactate dehydrogenase assays), AgNPs of 4·7 nm were much more toxic than the large AgNPs (42 nm). The cytotoxicity of both AgNPs was greatly decreased by pre-treatment with the antioxidant N-acetyl-L-cysteine. The oxidative stress parameters showed significant increase in reactive oxygen species levels, depletion of glutathione level and slight, but not statistically significant inactivation of superoxide dismutase, suggesting generation of oxidative stress. Thus, AgNPs should be used with caution for the topical treatment of burns and wounds, medical devices etc, because their toxicity depends on the size, the smaller NPs being much more cytotoxic than the large. SN - 1742-481X UR - https://www.unboundmedicine.com/medline/citation/24612846/Interactions_of_manufactured_silver_nanoparticles_of_different_sizes_with_normal_human_dermal_fibroblasts_ L2 - https://doi.org/10.1111/iwj.12244 DB - PRIME DP - Unbound Medicine ER -