Tags

Type your tag names separated by a space and hit enter

Neuroprotective effects of sevoflurane against electromagnetic pulse-induced brain injury through inhibition of neuronal oxidative stress and apoptosis.
PLoS One. 2014; 9(3):e91019.Plos

Abstract

Electromagnetic pulse (EMP) causes central nervous system damage and neurobehavioral disorders, and sevoflurane protects the brain from ischemic injury. We investigated the effects of sevoflurane on EMP-induced brain injury. Rats were exposed to EMP and immediately treated with sevoflurane. The protective effects of sevoflurane were assessed by Nissl staining, Fluoro-Jade C staining and electron microscopy. The neurobehavioral effects were assessed using the open-field test and the Morris water maze. Finally, primary cerebral cortical neurons were exposed to EMP and incubated with different concentration of sevoflurane. The cellular viability, lactate dehydrogenase (LDH) release, superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were assayed. TUNEL staining was performed, and the expression of apoptotic markers was determined. The cerebral cortexes of EMP-exposed rats presented neuronal abnormalities. Sevoflurane alleviated these effects, as well as the learning and memory deficits caused by EMP exposure. In vitro, cell viability was reduced and LDH release was increased after EMP exposure; treatment with sevoflurane ameliorated these effects. Additionally, sevoflurane increased SOD activity, decreased MDA levels and alleviated neuronal apoptosis by regulating the expression of cleaved caspase-3, Bax and Bcl-2. These findings demonstrate that Sevoflurane conferred neuroprotective effects against EMP radiation-induced brain damage by inhibiting neuronal oxidative stress and apoptosis.

Authors+Show Affiliations

State Key Laboratory of Military Stomatology, Department of Anesthesiology, School of Stomatology, The Fourth Military Medical University, Xi'an, China.Department of Anatomy, Histology and Embryology, K. K. Leung Brain Research Centre, the Fourth Military Medical University, Xi'an, China.State Key Laboratory of Military Stomatology, Department of Anesthesiology, School of Stomatology, The Fourth Military Medical University, Xi'an, China.Department of Radiation Medicine, Faculty of Preventive Medicine, the Fourth Military Medical University, Xi'an, China.Department of Anesthesiology, Shannxi Tumor Hospital, Xi'an, China.Department of Anesthesiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.Department of Nephrology, Bethune International Peace Hospital of People?'s Liberation Army, Shijiazhuang, China.Department of Clinical Pharmacology, Bethune International Peace Hospital of People's Liberation Army, Shijiazhuang, China.Department of Radiation Medicine, Faculty of Preventive Medicine, the Fourth Military Medical University, Xi'an, China.Department of Anesthesiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.Department of Clinical Pharmacology, Bethune International Peace Hospital of People's Liberation Army, Shijiazhuang, China; Department of Anesthesiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.State Key Laboratory of Military Stomatology, Department of Anesthesiology, School of Stomatology, The Fourth Military Medical University, Xi'an, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24614080

Citation

Deng, Bin, et al. "Neuroprotective Effects of Sevoflurane Against Electromagnetic Pulse-induced Brain Injury Through Inhibition of Neuronal Oxidative Stress and Apoptosis." PloS One, vol. 9, no. 3, 2014, pp. e91019.
Deng B, Xu H, Zhang J, et al. Neuroprotective effects of sevoflurane against electromagnetic pulse-induced brain injury through inhibition of neuronal oxidative stress and apoptosis. PLoS ONE. 2014;9(3):e91019.
Deng, B., Xu, H., Zhang, J., Wang, J., Han, L. C., Li, L. Y., Wu, G. L., Hou, Y. N., Guo, G. Z., Wang, Q., Sang, H. F., & Xu, L. X. (2014). Neuroprotective effects of sevoflurane against electromagnetic pulse-induced brain injury through inhibition of neuronal oxidative stress and apoptosis. PloS One, 9(3), e91019. https://doi.org/10.1371/journal.pone.0091019
Deng B, et al. Neuroprotective Effects of Sevoflurane Against Electromagnetic Pulse-induced Brain Injury Through Inhibition of Neuronal Oxidative Stress and Apoptosis. PLoS ONE. 2014;9(3):e91019. PubMed PMID: 24614080.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effects of sevoflurane against electromagnetic pulse-induced brain injury through inhibition of neuronal oxidative stress and apoptosis. AU - Deng,Bin, AU - Xu,Hao, AU - Zhang,Jin, AU - Wang,Jin, AU - Han,Li-Chun, AU - Li,Li-Ya, AU - Wu,Guang-Li, AU - Hou,Yan-Ning, AU - Guo,Guo-Zhen, AU - Wang,Qiang, AU - Sang,Han-Fei, AU - Xu,Li-Xian, Y1 - 2014/03/10/ PY - 2013/10/29/received PY - 2014/02/06/accepted PY - 2014/3/12/entrez PY - 2014/3/13/pubmed PY - 2016/4/1/medline SP - e91019 EP - e91019 JF - PloS one JO - PLoS ONE VL - 9 IS - 3 N2 - Electromagnetic pulse (EMP) causes central nervous system damage and neurobehavioral disorders, and sevoflurane protects the brain from ischemic injury. We investigated the effects of sevoflurane on EMP-induced brain injury. Rats were exposed to EMP and immediately treated with sevoflurane. The protective effects of sevoflurane were assessed by Nissl staining, Fluoro-Jade C staining and electron microscopy. The neurobehavioral effects were assessed using the open-field test and the Morris water maze. Finally, primary cerebral cortical neurons were exposed to EMP and incubated with different concentration of sevoflurane. The cellular viability, lactate dehydrogenase (LDH) release, superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were assayed. TUNEL staining was performed, and the expression of apoptotic markers was determined. The cerebral cortexes of EMP-exposed rats presented neuronal abnormalities. Sevoflurane alleviated these effects, as well as the learning and memory deficits caused by EMP exposure. In vitro, cell viability was reduced and LDH release was increased after EMP exposure; treatment with sevoflurane ameliorated these effects. Additionally, sevoflurane increased SOD activity, decreased MDA levels and alleviated neuronal apoptosis by regulating the expression of cleaved caspase-3, Bax and Bcl-2. These findings demonstrate that Sevoflurane conferred neuroprotective effects against EMP radiation-induced brain damage by inhibiting neuronal oxidative stress and apoptosis. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24614080/Neuroprotective_effects_of_sevoflurane_against_electromagnetic_pulse_induced_brain_injury_through_inhibition_of_neuronal_oxidative_stress_and_apoptosis_ L2 - http://dx.plos.org/10.1371/journal.pone.0091019 DB - PRIME DP - Unbound Medicine ER -