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Rapid determination of the amino acid configuration of xenotetrapeptide.
Chembiochem. 2014 Apr 14; 15(6):826-8.C

Abstract

An E. coli strain with deletions in five transaminases (ΔaspC ΔilvE ΔtyrB ΔavtA ΔybfQ) was constructed to be unable to degrade several amino acids. This strain was used as an expression host for the analysis of the amino acid configuration of nonribosomally synthesized peptides, including the novel peptide "xenotetrapeptide" from Xenorhabdus nematophila, by using a combination of labeling experiments and mass spectrometry. Additionally, the number of D-amino acids in the produced peptide was assigned following simple cultivation of the expression strain in D2 O.

Authors+Show Affiliations

Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany).No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24616055

Citation

Kegler, Carsten, et al. "Rapid Determination of the Amino Acid Configuration of Xenotetrapeptide." Chembiochem : a European Journal of Chemical Biology, vol. 15, no. 6, 2014, pp. 826-8.
Kegler C, Nollmann FI, Ahrendt T, et al. Rapid determination of the amino acid configuration of xenotetrapeptide. Chembiochem. 2014;15(6):826-8.
Kegler, C., Nollmann, F. I., Ahrendt, T., Fleischhacker, F., Bode, E., & Bode, H. B. (2014). Rapid determination of the amino acid configuration of xenotetrapeptide. Chembiochem : a European Journal of Chemical Biology, 15(6), 826-8. https://doi.org/10.1002/cbic.201300602
Kegler C, et al. Rapid Determination of the Amino Acid Configuration of Xenotetrapeptide. Chembiochem. 2014 Apr 14;15(6):826-8. PubMed PMID: 24616055.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid determination of the amino acid configuration of xenotetrapeptide. AU - Kegler,Carsten, AU - Nollmann,Friederike I, AU - Ahrendt,Tilman, AU - Fleischhacker,Florian, AU - Bode,Edna, AU - Bode,Helge B, Y1 - 2014/03/11/ PY - 2013/09/18/received PY - 2014/01/15/revised PY - 2014/3/12/entrez PY - 2014/3/13/pubmed PY - 2014/12/15/medline KW - D-amino acids KW - absolute configuration KW - deuterium exchange KW - mass spectrometry KW - peptides KW - structure elucidation SP - 826 EP - 8 JF - Chembiochem : a European journal of chemical biology JO - Chembiochem VL - 15 IS - 6 N2 - An E. coli strain with deletions in five transaminases (ΔaspC ΔilvE ΔtyrB ΔavtA ΔybfQ) was constructed to be unable to degrade several amino acids. This strain was used as an expression host for the analysis of the amino acid configuration of nonribosomally synthesized peptides, including the novel peptide "xenotetrapeptide" from Xenorhabdus nematophila, by using a combination of labeling experiments and mass spectrometry. Additionally, the number of D-amino acids in the produced peptide was assigned following simple cultivation of the expression strain in D2 O. SN - 1439-7633 UR - https://www.unboundmedicine.com/medline/citation/24616055/Rapid_determination_of_the_amino_Acid_configuration_of_xenotetrapeptide_ L2 - https://doi.org/10.1002/cbic.201300602 DB - PRIME DP - Unbound Medicine ER -