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Identification of proteins associated with ion homeostasis and salt tolerance in barley.
Proteomics. 2014 Jun; 14(11):1381-92.P

Abstract

Identification and characterization of proteins involved in salt tolerance are imperative for revealing its genetic mechanisms. In this study, ionic and proteomic responses of a Tibetan wild barley XZ16 and a well-known salt-tolerant barley cv. CM72 were analyzed using inductively coupled plasma-optical emission spectrometer, 2DE, and MALDI-TOF/TOF MS techniques to determine salt-induced differences in element and protein profiles between the two genotypes. In total, 41 differentially expressed proteins were identified in roots and leaves, and they were associated with ion homeostasis, cell redox homeostasis, metabolic process, and photosynthesis. Under salinity stress, calmodulin, Na/K transporters, and H(+) -ATPases were involved in establishment of ion homeostasis for barley plants. Moreover, ribulose-1,5-bisphosphate carboxylase/oxygenase activase and oxygen-evolving enhancer proteins were significantly upregulated under salinity stress, indicating the great impact of salinity on photosynthesis. In comparison with CM72, XZ16 had greater relative dry weight and lower Na accumulation in the shoots under salinity stress. A higher expression of HvNHX1 in the roots, and some specific proteins responsible for ion homeostasis and cell redox homeostasis, was also found in XZ16 exposed to salt stress. The current results showed that Tibetan wild barley XZ16 and cultivated barley cultivar CM72 differ in the mechanism of salt tolerance.

Authors+Show Affiliations

Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Department of Agronomy, Zhejiang University, Hangzhou, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24616274

Citation

Wu, Dezhi, et al. "Identification of Proteins Associated With Ion Homeostasis and Salt Tolerance in Barley." Proteomics, vol. 14, no. 11, 2014, pp. 1381-92.
Wu D, Shen Q, Qiu L, et al. Identification of proteins associated with ion homeostasis and salt tolerance in barley. Proteomics. 2014;14(11):1381-92.
Wu, D., Shen, Q., Qiu, L., Han, Y., Ye, L., Jabeen, Z., Shu, Q., & Zhang, G. (2014). Identification of proteins associated with ion homeostasis and salt tolerance in barley. Proteomics, 14(11), 1381-92. https://doi.org/10.1002/pmic.201300221
Wu D, et al. Identification of Proteins Associated With Ion Homeostasis and Salt Tolerance in Barley. Proteomics. 2014;14(11):1381-92. PubMed PMID: 24616274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of proteins associated with ion homeostasis and salt tolerance in barley. AU - Wu,Dezhi, AU - Shen,Qiufang, AU - Qiu,Long, AU - Han,Yong, AU - Ye,Linzheng, AU - Jabeen,Zahra, AU - Shu,Qingyao, AU - Zhang,Guoping, Y1 - 2014/04/16/ PY - 2013/06/04/received PY - 2014/01/24/revised PY - 2014/03/04/accepted PY - 2014/3/12/entrez PY - 2014/3/13/pubmed PY - 2015/1/23/medline KW - Hordeum vulgare and Hordeum spontaneum KW - Ion homeostasis KW - Plant proteomics KW - Salt tolerance SP - 1381 EP - 92 JF - Proteomics JO - Proteomics VL - 14 IS - 11 N2 - Identification and characterization of proteins involved in salt tolerance are imperative for revealing its genetic mechanisms. In this study, ionic and proteomic responses of a Tibetan wild barley XZ16 and a well-known salt-tolerant barley cv. CM72 were analyzed using inductively coupled plasma-optical emission spectrometer, 2DE, and MALDI-TOF/TOF MS techniques to determine salt-induced differences in element and protein profiles between the two genotypes. In total, 41 differentially expressed proteins were identified in roots and leaves, and they were associated with ion homeostasis, cell redox homeostasis, metabolic process, and photosynthesis. Under salinity stress, calmodulin, Na/K transporters, and H(+) -ATPases were involved in establishment of ion homeostasis for barley plants. Moreover, ribulose-1,5-bisphosphate carboxylase/oxygenase activase and oxygen-evolving enhancer proteins were significantly upregulated under salinity stress, indicating the great impact of salinity on photosynthesis. In comparison with CM72, XZ16 had greater relative dry weight and lower Na accumulation in the shoots under salinity stress. A higher expression of HvNHX1 in the roots, and some specific proteins responsible for ion homeostasis and cell redox homeostasis, was also found in XZ16 exposed to salt stress. The current results showed that Tibetan wild barley XZ16 and cultivated barley cultivar CM72 differ in the mechanism of salt tolerance. SN - 1615-9861 UR - https://www.unboundmedicine.com/medline/citation/24616274/Identification_of_proteins_associated_with_ion_homeostasis_and_salt_tolerance_in_barley_ DB - PRIME DP - Unbound Medicine ER -