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Cytotoxicity and apoptosis induced by alfalfa (Medicago sativa) leaf extracts in sensitive and multidrug-resistant tumor cells.
Nutr Cancer. 2014; 66(3):483-91.NC

Abstract

Alfalfa (Medicago sativa) has been used to cure a wide variety of ailments. However, only a few studies have reported its anticancer effects. In this study, extracts were obtained from alfalfa leaves and their cytotoxic effects were assessed on several sensitive and multidrug-resistant tumor cells lines. Using the mouse leukaemia P388 cell line and its doxorubicin-resistant counterpart (P388/DOX), we showed that the inhibition of cell growth induced by alfalfa leaf extracts was mediated through the induction of apoptosis, as evidenced by DNA fragmentation analysis. The execution of programmed cell death was achieved via the activation of caspase-3, leading to PARP cleavage. Fractionation of toluene extract (To-1), the most active extract obtained from crude extract, led to the identification of 3 terpene derivatives and 5 flavonoids. Among them, (-)-medicarpin, (-)-melilotocarpan E, millepurpan, tricin, and chrysoeriol showed cytotoxic effects in P388 as well as P388/DOX cells. These results demonstrate that alfalfa leaf extract may have interesting potential in cancer chemoprevention and therapy.

Authors+Show Affiliations

a Laboratoire de Biochimie et Biologie Moléculaire , Faculté de Pharmacie, URCA , Reims , France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24628411

Citation

Gatouillat, Grégory, et al. "Cytotoxicity and Apoptosis Induced By Alfalfa (Medicago Sativa) Leaf Extracts in Sensitive and Multidrug-resistant Tumor Cells." Nutrition and Cancer, vol. 66, no. 3, 2014, pp. 483-91.
Gatouillat G, Magid AA, Bertin E, et al. Cytotoxicity and apoptosis induced by alfalfa (Medicago sativa) leaf extracts in sensitive and multidrug-resistant tumor cells. Nutr Cancer. 2014;66(3):483-91.
Gatouillat, G., Magid, A. A., Bertin, E., Okiemy-Akeli, M. G., Morjani, H., Lavaud, C., & Madoulet, C. (2014). Cytotoxicity and apoptosis induced by alfalfa (Medicago sativa) leaf extracts in sensitive and multidrug-resistant tumor cells. Nutrition and Cancer, 66(3), 483-91. https://doi.org/10.1080/01635581.2014.884228
Gatouillat G, et al. Cytotoxicity and Apoptosis Induced By Alfalfa (Medicago Sativa) Leaf Extracts in Sensitive and Multidrug-resistant Tumor Cells. Nutr Cancer. 2014;66(3):483-91. PubMed PMID: 24628411.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxicity and apoptosis induced by alfalfa (Medicago sativa) leaf extracts in sensitive and multidrug-resistant tumor cells. AU - Gatouillat,Grégory, AU - Magid,Abdulmagid Alabdul, AU - Bertin,Eric, AU - Okiemy-Akeli,Marie-Genevieve, AU - Morjani,Hamid, AU - Lavaud,Catherine, AU - Madoulet,Claudie, Y1 - 2014/03/14/ PY - 2014/3/18/entrez PY - 2014/3/19/pubmed PY - 2015/5/27/medline SP - 483 EP - 91 JF - Nutrition and cancer JO - Nutr Cancer VL - 66 IS - 3 N2 - Alfalfa (Medicago sativa) has been used to cure a wide variety of ailments. However, only a few studies have reported its anticancer effects. In this study, extracts were obtained from alfalfa leaves and their cytotoxic effects were assessed on several sensitive and multidrug-resistant tumor cells lines. Using the mouse leukaemia P388 cell line and its doxorubicin-resistant counterpart (P388/DOX), we showed that the inhibition of cell growth induced by alfalfa leaf extracts was mediated through the induction of apoptosis, as evidenced by DNA fragmentation analysis. The execution of programmed cell death was achieved via the activation of caspase-3, leading to PARP cleavage. Fractionation of toluene extract (To-1), the most active extract obtained from crude extract, led to the identification of 3 terpene derivatives and 5 flavonoids. Among them, (-)-medicarpin, (-)-melilotocarpan E, millepurpan, tricin, and chrysoeriol showed cytotoxic effects in P388 as well as P388/DOX cells. These results demonstrate that alfalfa leaf extract may have interesting potential in cancer chemoprevention and therapy. SN - 1532-7914 UR - https://www.unboundmedicine.com/medline/citation/24628411/Cytotoxicity_and_apoptosis_induced_by_alfalfa__Medicago_sativa__leaf_extracts_in_sensitive_and_multidrug_resistant_tumor_cells_ L2 - http://www.tandfonline.com/doi/full/10.1080/01635581.2014.884228 DB - PRIME DP - Unbound Medicine ER -