Tags

Type your tag names separated by a space and hit enter

Matrine induces caspase-independent program cell death in hepatocellular carcinoma through bid-mediated nuclear translocation of apoptosis inducing factor.
Mol Cancer. 2014 Mar 16; 13:59.MC

Abstract

Matrine, a clinical drug in China, has been used to treat viral hepatitis, cardiac arrhythmia and skin inflammations. Matrine also exhibits chemotherapeutic potential through its ability to trigger cancer cell death. However, the mechanisms involved are still largely unknown. The objective of this study was to investigate the major determinant for the cell death induced by matrine in human hepatocellular carcinoma. We use human hepatocellular carcinoma cell line HepG2 and human hepatocellular carcinoma xenograft in nude mice as models to study the action of matrine in hepatocellular cancers. We found that caspase-dependent and -independent Program Cell Death (PCD) occurred in matrine-treated HepG2 cells, accompanied by the decreasing of mitochondrial transmembrane potential and the increasing ROS production. Further studies showed that AIF released from the mitochondria to the nucleus, and silencing of AIF reduced the caspase-independent PCD induced by matrine. What's more, AIF nuclear translocation, and the subsequent cell death as well, was prevented by Bid inhibitor BI-6C9, Bid-targeted siRNA and ROS scavenger Tiron. In the in vivo study, matrine significantly attenuated tumor growth with AIF release from mitochondria into nucleus in nude mice. These data imply that matrine potently induce caspase-independent PCD in HepG2 cells through Bid-mediated AIF translocation.

Authors+Show Affiliations

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableCancer Research Center, Xiamen University Medical college, Xiamen 361102, China. gangsongsd@xmu.edu.cn.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24628719

Citation

Zhou, Huan, et al. "Matrine Induces Caspase-independent Program Cell Death in Hepatocellular Carcinoma Through Bid-mediated Nuclear Translocation of Apoptosis Inducing Factor." Molecular Cancer, vol. 13, 2014, p. 59.
Zhou H, Xu M, Gao Y, et al. Matrine induces caspase-independent program cell death in hepatocellular carcinoma through bid-mediated nuclear translocation of apoptosis inducing factor. Mol Cancer. 2014;13:59.
Zhou, H., Xu, M., Gao, Y., Deng, Z., Cao, H., Zhang, W., Wang, Q., Zhang, B., Song, G., Zhan, Y., & Hu, T. (2014). Matrine induces caspase-independent program cell death in hepatocellular carcinoma through bid-mediated nuclear translocation of apoptosis inducing factor. Molecular Cancer, 13, 59. https://doi.org/10.1186/1476-4598-13-59
Zhou H, et al. Matrine Induces Caspase-independent Program Cell Death in Hepatocellular Carcinoma Through Bid-mediated Nuclear Translocation of Apoptosis Inducing Factor. Mol Cancer. 2014 Mar 16;13:59. PubMed PMID: 24628719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrine induces caspase-independent program cell death in hepatocellular carcinoma through bid-mediated nuclear translocation of apoptosis inducing factor. AU - Zhou,Huan, AU - Xu,Minying, AU - Gao,Ya, AU - Deng,Zhigang, AU - Cao,Hanwei, AU - Zhang,Wenqing, AU - Wang,Qiao, AU - Zhang,Bing, AU - Song,Gang, AU - Zhan,Yanyan, AU - Hu,Tianhui, Y1 - 2014/03/16/ PY - 2013/12/11/received PY - 2014/03/10/accepted PY - 2014/3/18/entrez PY - 2014/3/19/pubmed PY - 2014/9/10/medline SP - 59 EP - 59 JF - Molecular cancer JO - Mol Cancer VL - 13 N2 - Matrine, a clinical drug in China, has been used to treat viral hepatitis, cardiac arrhythmia and skin inflammations. Matrine also exhibits chemotherapeutic potential through its ability to trigger cancer cell death. However, the mechanisms involved are still largely unknown. The objective of this study was to investigate the major determinant for the cell death induced by matrine in human hepatocellular carcinoma. We use human hepatocellular carcinoma cell line HepG2 and human hepatocellular carcinoma xenograft in nude mice as models to study the action of matrine in hepatocellular cancers. We found that caspase-dependent and -independent Program Cell Death (PCD) occurred in matrine-treated HepG2 cells, accompanied by the decreasing of mitochondrial transmembrane potential and the increasing ROS production. Further studies showed that AIF released from the mitochondria to the nucleus, and silencing of AIF reduced the caspase-independent PCD induced by matrine. What's more, AIF nuclear translocation, and the subsequent cell death as well, was prevented by Bid inhibitor BI-6C9, Bid-targeted siRNA and ROS scavenger Tiron. In the in vivo study, matrine significantly attenuated tumor growth with AIF release from mitochondria into nucleus in nude mice. These data imply that matrine potently induce caspase-independent PCD in HepG2 cells through Bid-mediated AIF translocation. SN - 1476-4598 UR - https://www.unboundmedicine.com/medline/citation/24628719/Matrine_induces_caspase_independent_program_cell_death_in_hepatocellular_carcinoma_through_bid_mediated_nuclear_translocation_of_apoptosis_inducing_factor_ L2 - https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-59 DB - PRIME DP - Unbound Medicine ER -