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Protective effect of tea polyphenols on renal ischemia/reperfusion injury via suppressing the activation of TLR4/NF-κB p65 signal pathway.
Gene. 2014 May 25; 542(1):46-51.GENE

Abstract

Tea polyphenols (TP) was investigated in rats for its protective effect on renal ischemia/reperfusion injury (RIRI). Rats were randomized into groups as follows: (I) sham group (n=10); (II) RIRI group (n=10); (III) RIRI+TP (100mg/kg) group (n=5); (IV) RIRI+TP (200mg/kg) group (n=5); (V) RIRI+TP+ Astragalus mongholicus aqueous extract (AMAE) (300 mg/kg+100mg/kg) group (n=5). For the IRI+TP groups, rats were orally given with tea polyphenols (100, 200 and 300 mg/kg body weight) once daily 10 days before induction of ischemia, followed by renal IRI. For the sham group and RIRI group, rats were orally given with equal volume of saline once daily 10 days before induction of ischemia, followed by renal IRI. Results showed that tea polyphenol pretreatment significantly suppressed ROS level and MDA release. On the other hand, in rats subjected to ischemia-reperfusion, the activities of endogenous antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and glutathione peroxidase (GSH-Px) showed recovery, whereas the levels of urea nitrogen and serum creatinine were reduced by administration of tea polyphenols orally for 10 days prior to ischemia-reperfusion. Moreover, tea polyphenol pretreatment significantly decreased TLR4 and NF-κB p65 protein expression levels in RIRI rats. At the same time, tea polyphenol pretreatment attenuated the increased level of serum IL-1β, IL-6, ICAM-1 and TNF-α, and enhanced IL-10 production in RIRI rats. Furthermore, tea polyphenol pretreatment significantly decreased renal epithelial tubular cell apoptosis induced by renal ischemia/reperfusion, alleviating renal ischemia/reperfusion injury. These results cumulatively indicate that tea polyphenol pretreatment could suppress the TLR4/NF-κB p65 signaling pathway, protecting renal tubular epithelial cells against ischemia/reperfusion-induced apoptosis, which implies that antioxidants may be a potential and effective agent for prevention of the ischemic/reperfusion injury through the suppression extrinsic apoptotic signal pathway induced by TLR4/NF-κB p65 signal pathway. Moreover, supplement of AMAE can increased renal protection effect of TP.

Authors+Show Affiliations

Department of Nephrology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300060, China. Electronic address: yzhangtj@yeah.net.Department of Pharmacy, Tianjin Huanhu Hospital, Tianjin 300060, China.Tianjin institute of medical and pharmaceutical sciences, Tianjin 300000, China.Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin 300100, China.Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300060, China.Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300060, China.Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300060, China.Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300060, China.Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300060, China.Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300060, China.Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300060, China.Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300060, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24630969

Citation

Li, Yan-Wei, et al. "Protective Effect of Tea Polyphenols On Renal Ischemia/reperfusion Injury Via Suppressing the Activation of TLR4/NF-κB P65 Signal Pathway." Gene, vol. 542, no. 1, 2014, pp. 46-51.
Li YW, Zhang Y, Zhang L, et al. Protective effect of tea polyphenols on renal ischemia/reperfusion injury via suppressing the activation of TLR4/NF-κB p65 signal pathway. Gene. 2014;542(1):46-51.
Li, Y. W., Zhang, Y., Zhang, L., Li, X., Yu, J. B., Zhang, H. T., Tan, B. B., Jiang, L. H., Wang, Y. X., Liang, Y., Zhang, X. S., Wang, W. S., & Liu, H. G. (2014). Protective effect of tea polyphenols on renal ischemia/reperfusion injury via suppressing the activation of TLR4/NF-κB p65 signal pathway. Gene, 542(1), 46-51. https://doi.org/10.1016/j.gene.2014.03.021
Li YW, et al. Protective Effect of Tea Polyphenols On Renal Ischemia/reperfusion Injury Via Suppressing the Activation of TLR4/NF-κB P65 Signal Pathway. Gene. 2014 May 25;542(1):46-51. PubMed PMID: 24630969.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of tea polyphenols on renal ischemia/reperfusion injury via suppressing the activation of TLR4/NF-κB p65 signal pathway. AU - Li,Yan-Wei, AU - Zhang,Yan, AU - Zhang,Ling, AU - Li,Xu, AU - Yu,Jian-Bo, AU - Zhang,Hong-Tao, AU - Tan,Bin-Bin, AU - Jiang,Lian-Hao, AU - Wang,Ya-Xin, AU - Liang,Yu, AU - Zhang,Xiu-Shan, AU - Wang,Wen-Sheng, AU - Liu,Hai-Gen, Y1 - 2014/03/12/ PY - 2013/11/19/received PY - 2014/03/06/revised PY - 2014/03/10/accepted PY - 2014/3/18/entrez PY - 2014/3/19/pubmed PY - 2014/6/6/medline KW - Antioxidant KW - Astragalus mongholicus KW - Rat KW - Renal ischemia–reperfusion KW - TLR4/NF-κB p65 signal pathway KW - Tea polyphenols SP - 46 EP - 51 JF - Gene JO - Gene VL - 542 IS - 1 N2 - Tea polyphenols (TP) was investigated in rats for its protective effect on renal ischemia/reperfusion injury (RIRI). Rats were randomized into groups as follows: (I) sham group (n=10); (II) RIRI group (n=10); (III) RIRI+TP (100mg/kg) group (n=5); (IV) RIRI+TP (200mg/kg) group (n=5); (V) RIRI+TP+ Astragalus mongholicus aqueous extract (AMAE) (300 mg/kg+100mg/kg) group (n=5). For the IRI+TP groups, rats were orally given with tea polyphenols (100, 200 and 300 mg/kg body weight) once daily 10 days before induction of ischemia, followed by renal IRI. For the sham group and RIRI group, rats were orally given with equal volume of saline once daily 10 days before induction of ischemia, followed by renal IRI. Results showed that tea polyphenol pretreatment significantly suppressed ROS level and MDA release. On the other hand, in rats subjected to ischemia-reperfusion, the activities of endogenous antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and glutathione peroxidase (GSH-Px) showed recovery, whereas the levels of urea nitrogen and serum creatinine were reduced by administration of tea polyphenols orally for 10 days prior to ischemia-reperfusion. Moreover, tea polyphenol pretreatment significantly decreased TLR4 and NF-κB p65 protein expression levels in RIRI rats. At the same time, tea polyphenol pretreatment attenuated the increased level of serum IL-1β, IL-6, ICAM-1 and TNF-α, and enhanced IL-10 production in RIRI rats. Furthermore, tea polyphenol pretreatment significantly decreased renal epithelial tubular cell apoptosis induced by renal ischemia/reperfusion, alleviating renal ischemia/reperfusion injury. These results cumulatively indicate that tea polyphenol pretreatment could suppress the TLR4/NF-κB p65 signaling pathway, protecting renal tubular epithelial cells against ischemia/reperfusion-induced apoptosis, which implies that antioxidants may be a potential and effective agent for prevention of the ischemic/reperfusion injury through the suppression extrinsic apoptotic signal pathway induced by TLR4/NF-κB p65 signal pathway. Moreover, supplement of AMAE can increased renal protection effect of TP. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/24630969/Protective_effect_of_tea_polyphenols_on_renal_ischemia/reperfusion_injury_via_suppressing_the_activation_of_TLR4/NF_κB_p65_signal_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(14)00299-6 DB - PRIME DP - Unbound Medicine ER -